Experiment: LB + carbenicillin Time1-2hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliR and rcsA are separated by 289 nucleotides rcsA and dsrB are separated by 43 nucleotides dsrB and b1953 are separated by 162 nucleotides b1953 and dsrA are separated by 13 nucleotides dsrA and yedP are separated by 197 nucleotides
b1950: fliR - flagellar biosynthesis protein R (NCBI), at 2,020,917 to 2,021,702
fliR
b1951: rcsA - DNA-binding transcriptional activator, co-regulator with RcsB (NCBI), at 2,021,992 to 2,022,615
rcsA
b1952: dsrB - hypothetical protein (NCBI), at 2,022,659 to 2,022,847
dsrB
b1953: b1953 - orf, hypothetical protein (VIMSS), at 2,023,010 to 2,023,237
b1953
b1954: dsrA - regulatory antisense RNA (NCBI), at 2,023,251 to 2,023,337
dsrA
b1955: yedP - hypothetical protein (NCBI), at 2,023,535 to 2,024,350
yedP
Position (kb)
2021
2022
2023 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2021.298 kb on + strand, within fliR at 2021.377 kb on + strand, within fliR at 2021.377 kb on + strand, within fliR at 2021.413 kb on - strand, within fliR at 2021.498 kb on + strand, within fliR at 2021.501 kb on + strand, within fliR at 2021.501 kb on + strand, within fliR at 2021.522 kb on - strand, within fliR at 2021.572 kb on + strand, within fliR at 2021.594 kb on + strand, within fliR at 2021.594 kb on + strand, within fliR at 2021.595 kb on + strand, within fliR at 2021.595 kb on + strand, within fliR at 2021.812 kb on - strand at 2021.982 kb on + strand at 2021.982 kb on + strand at 2022.049 kb on + strand at 2022.191 kb on + strand, within rcsA at 2022.382 kb on - strand, within rcsA at 2022.382 kb on - strand, within rcsA at 2022.413 kb on - strand, within rcsA at 2022.413 kb on - strand, within rcsA at 2022.424 kb on - strand, within rcsA at 2022.424 kb on - strand, within rcsA at 2022.427 kb on - strand, within rcsA at 2022.435 kb on + strand, within rcsA at 2022.453 kb on - strand, within rcsA at 2022.456 kb on - strand, within rcsA at 2022.610 kb on + strand at 2022.610 kb on + strand at 2022.627 kb on - strand at 2022.637 kb on - strand at 2022.650 kb on - strand at 2022.650 kb on - strand at 2022.674 kb on - strand at 2022.674 kb on - strand at 2022.694 kb on + strand, within dsrB at 2022.694 kb on + strand, within dsrB at 2022.720 kb on - strand, within dsrB at 2022.770 kb on + strand, within dsrB at 2022.770 kb on + strand, within dsrB at 2022.787 kb on - strand, within dsrB at 2022.787 kb on - strand, within dsrB at 2022.790 kb on + strand, within dsrB at 2022.792 kb on - strand, within dsrB at 2022.874 kb on + strand at 2022.874 kb on + strand at 2022.874 kb on + strand at 2022.940 kb on + strand at 2022.942 kb on + strand at 2023.038 kb on - strand, within b1953 at 2023.107 kb on + strand, within b1953 at 2023.138 kb on + strand, within b1953 at 2023.138 kb on + strand, within b1953 at 2023.138 kb on + strand, within b1953 at 2023.267 kb on - strand, within dsrA at 2023.298 kb on + strand, within dsrA at 2023.343 kb on - strand at 2023.343 kb on - strand at 2023.439 kb on - strand at 2023.439 kb on - strand at 2023.517 kb on + strand at 2023.525 kb on - strand at 2023.525 kb on - strand at 2023.540 kb on - strand at 2023.540 kb on - strand at 2023.604 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr remove 2,021,298 + fliR b1950 0.48 -0.3 2,021,377 + fliR b1950 0.59 -2.0 2,021,377 + fliR b1950 0.59 -1.1 2,021,413 - fliR b1950 0.63 +0.5 2,021,498 + fliR b1950 0.74 -0.1 2,021,501 + fliR b1950 0.74 +0.5 2,021,501 + fliR b1950 0.74 -0.4 2,021,522 - fliR b1950 0.77 +1.5 2,021,572 + fliR b1950 0.83 +1.5 2,021,594 + fliR b1950 0.86 +1.5 2,021,594 + fliR b1950 0.86 +0.5 2,021,595 + fliR b1950 0.86 +1.5 2,021,595 + fliR b1950 0.86 -1.1 2,021,812 - +1.5 2,021,982 + +1.5 2,021,982 + +1.5 2,022,049 + -0.8 2,022,191 + rcsA b1951 0.32 +1.5 2,022,382 - rcsA b1951 0.62 +1.5 2,022,382 - rcsA b1951 0.62 -0.1 2,022,413 - rcsA b1951 0.67 +0.5 2,022,413 - rcsA b1951 0.67 +1.5 2,022,424 - rcsA b1951 0.69 +1.5 2,022,424 - rcsA b1951 0.69 +1.5 2,022,427 - rcsA b1951 0.70 -0.1 2,022,435 + rcsA b1951 0.71 +1.5 2,022,453 - rcsA b1951 0.74 +1.1 2,022,456 - rcsA b1951 0.74 +2.0 2,022,610 + +1.5 2,022,610 + +0.9 2,022,627 - -0.5 2,022,637 - -0.5 2,022,650 - +0.1 2,022,650 - -0.9 2,022,674 - -0.1 2,022,674 - +1.5 2,022,694 + dsrB b1952 0.19 -0.5 2,022,694 + dsrB b1952 0.19 +1.5 2,022,720 - dsrB b1952 0.32 -0.2 2,022,770 + dsrB b1952 0.59 -0.2 2,022,770 + dsrB b1952 0.59 -0.0 2,022,787 - dsrB b1952 0.68 +0.2 2,022,787 - dsrB b1952 0.68 -0.1 2,022,790 + dsrB b1952 0.69 +0.4 2,022,792 - dsrB b1952 0.70 -0.3 2,022,874 + -0.3 2,022,874 + -0.3 2,022,874 + +0.9 2,022,940 + +0.4 2,022,942 + +0.0 2,023,038 - b1953 b1953 0.12 -0.2 2,023,107 + b1953 b1953 0.43 +0.0 2,023,138 + b1953 b1953 0.56 -0.5 2,023,138 + b1953 b1953 0.56 -0.2 2,023,138 + b1953 b1953 0.56 +0.3 2,023,267 - dsrA b1954 0.18 +0.2 2,023,298 + dsrA b1954 0.54 +0.4 2,023,343 - +0.1 2,023,343 - -0.2 2,023,439 - +0.3 2,023,439 - -0.2 2,023,517 + +0.3 2,023,525 - +0.5 2,023,525 - -0.0 2,023,540 - -0.2 2,023,540 - -0.2 2,023,604 - +0.5
Or see this region's nucleotide sequence