Strain Fitness in Escherichia coli BW25113 around b1121

Experiment: LB + carbenicillin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnagK and cobB are separated by 126 nucleotidescobB and ycfZ are separated by 119 nucleotidesycfZ and ymfA overlap by 4 nucleotidesymfA and potD are separated by 57 nucleotides b1119: nagK - N-acetyl-D-glucosamine kinase (NCBI), at 1,177,816 to 1,178,727 nagK b1120: cobB - deacetylase of acs and cheY, regulates chemotaxis (NCBI), at 1,178,854 to 1,179,582 cobB b1121: ycfZ - predicted inner membrane protein (NCBI), at 1,179,702 to 1,180,490 ycfZ b1122: ymfA - orf, hypothetical protein (VIMSS), at 1,180,487 to 1,180,948 ymfA b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052 potD Position (kb) 1179 1180 1181Strain fitness (log2 ratio) -1 0 1at 1178.708 kb on + strandat 1178.708 kb on + strandat 1178.766 kb on + strandat 1178.766 kb on + strandat 1178.875 kb on + strandat 1178.875 kb on + strandat 1178.880 kb on - strandat 1178.880 kb on - strandat 1179.036 kb on - strand, within cobBat 1179.142 kb on + strand, within cobBat 1179.161 kb on + strand, within cobBat 1179.161 kb on + strand, within cobBat 1179.195 kb on - strand, within cobBat 1179.319 kb on + strand, within cobBat 1179.396 kb on + strand, within cobBat 1179.413 kb on + strand, within cobBat 1179.413 kb on + strand, within cobBat 1179.421 kb on - strand, within cobBat 1179.526 kb on + strandat 1179.552 kb on - strandat 1179.584 kb on + strandat 1179.584 kb on + strandat 1179.584 kb on + strandat 1179.594 kb on + strandat 1179.758 kb on - strandat 1179.768 kb on - strandat 1179.785 kb on + strand, within ycfZat 1179.833 kb on + strand, within ycfZat 1179.837 kb on - strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.943 kb on + strand, within ycfZat 1179.992 kb on + strand, within ycfZat 1180.234 kb on - strand, within ycfZat 1180.389 kb on - strand, within ycfZat 1180.389 kb on - strand, within ycfZat 1180.438 kb on + strandat 1180.457 kb on + strandat 1180.739 kb on - strand, within ymfAat 1180.755 kb on + strand, within ymfAat 1180.755 kb on - strand, within ymfAat 1180.755 kb on - strand, within ymfAat 1180.763 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.778 kb on - strand, within ymfAat 1180.796 kb on + strand, within ymfAat 1180.796 kb on + strand, within ymfAat 1180.799 kb on - strand, within ymfAat 1180.800 kb on - strand, within ymfAat 1180.803 kb on - strand, within ymfAat 1180.809 kb on + strand, within ymfAat 1180.813 kb on + strand, within ymfAat 1180.817 kb on - strand, within ymfAat 1180.834 kb on - strand, within ymfAat 1180.838 kb on - strand, within ymfAat 1180.854 kb on - strand, within ymfAat 1180.869 kb on - strand, within ymfAat 1180.881 kb on + strand, within ymfAat 1180.881 kb on + strand, within ymfAat 1180.890 kb on + strand, within ymfAat 1180.894 kb on + strand, within ymfAat 1180.894 kb on + strand, within ymfAat 1180.894 kb on + strand, within ymfAat 1180.900 kb on + strand, within ymfAat 1180.900 kb on + strand, within ymfAat 1180.902 kb on - strandat 1180.908 kb on - strandat 1180.937 kb on + strandat 1180.945 kb on - strandat 1180.945 kb on - strandat 1180.953 kb on - strandat 1180.959 kb on - strandat 1180.988 kb on - strandat 1180.988 kb on - strandat 1180.989 kb on - strandat 1180.989 kb on - strandat 1181.036 kb on - strandat 1181.046 kb on - strandat 1181.101 kb on + strandat 1181.101 kb on + strandat 1181.170 kb on - strand, within potDat 1181.280 kb on - strand, within potDat 1181.280 kb on - strand, within potDat 1181.421 kb on + strand, within potDat 1181.480 kb on - strand, within potD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr
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1,178,708 + -0.7
1,178,708 + -0.2
1,178,766 + +0.7
1,178,766 + -0.1
1,178,875 + -0.7
1,178,875 + -0.3
1,178,880 - +1.2
1,178,880 - -0.1
1,179,036 - cobB b1120 0.25 -1.0
1,179,142 + cobB b1120 0.40 -0.0
1,179,161 + cobB b1120 0.42 +0.6
1,179,161 + cobB b1120 0.42 +0.4
1,179,195 - cobB b1120 0.47 -0.0
1,179,319 + cobB b1120 0.64 +0.1
1,179,396 + cobB b1120 0.74 +0.1
1,179,413 + cobB b1120 0.77 +0.7
1,179,413 + cobB b1120 0.77 -0.5
1,179,421 - cobB b1120 0.78 -0.0
1,179,526 + +0.6
1,179,552 - +1.5
1,179,584 + +0.3
1,179,584 + +0.3
1,179,584 + -0.5
1,179,594 + -0.1
1,179,758 - -0.1
1,179,768 - +0.9
1,179,785 + ycfZ b1121 0.11 +0.3
1,179,833 + ycfZ b1121 0.17 -0.2
1,179,837 - ycfZ b1121 0.17 -0.5
1,179,922 + ycfZ b1121 0.28 +1.5
1,179,922 + ycfZ b1121 0.28 +1.5
1,179,922 + ycfZ b1121 0.28 +1.5
1,179,922 + ycfZ b1121 0.28 +1.5
1,179,922 + ycfZ b1121 0.28 +1.5
1,179,943 + ycfZ b1121 0.31 +1.5
1,179,992 + ycfZ b1121 0.37 +1.5
1,180,234 - ycfZ b1121 0.67 +1.5
1,180,389 - ycfZ b1121 0.87 +1.5
1,180,389 - ycfZ b1121 0.87 +1.5
1,180,438 + +1.5
1,180,457 + +1.5
1,180,739 - ymfA b1122 0.55 +0.2
1,180,755 + ymfA b1122 0.58 +1.5
1,180,755 - ymfA b1122 0.58 +0.1
1,180,755 - ymfA b1122 0.58 +1.1
1,180,763 - ymfA b1122 0.60 -0.9
1,180,777 - ymfA b1122 0.63 -1.0
1,180,777 - ymfA b1122 0.63 -1.0
1,180,777 - ymfA b1122 0.63 +1.0
1,180,777 - ymfA b1122 0.63 -0.5
1,180,777 - ymfA b1122 0.63 +0.7
1,180,777 - ymfA b1122 0.63 -0.5
1,180,778 - ymfA b1122 0.63 +0.1
1,180,796 + ymfA b1122 0.67 -0.1
1,180,796 + ymfA b1122 0.67 +0.5
1,180,799 - ymfA b1122 0.68 -0.2
1,180,800 - ymfA b1122 0.68 -0.1
1,180,803 - ymfA b1122 0.68 -0.1
1,180,809 + ymfA b1122 0.70 +1.1
1,180,813 + ymfA b1122 0.71 +0.6
1,180,817 - ymfA b1122 0.71 -0.4
1,180,834 - ymfA b1122 0.75 +0.1
1,180,838 - ymfA b1122 0.76 -1.1
1,180,854 - ymfA b1122 0.79 -0.3
1,180,869 - ymfA b1122 0.83 -0.4
1,180,881 + ymfA b1122 0.85 -0.4
1,180,881 + ymfA b1122 0.85 -0.0
1,180,890 + ymfA b1122 0.87 -0.5
1,180,894 + ymfA b1122 0.88 +0.1
1,180,894 + ymfA b1122 0.88 +0.2
1,180,894 + ymfA b1122 0.88 +0.3
1,180,900 + ymfA b1122 0.89 -0.4
1,180,900 + ymfA b1122 0.89 +0.4
1,180,902 - +0.6
1,180,908 - +1.0
1,180,937 + -0.4
1,180,945 - -0.5
1,180,945 - +1.5
1,180,953 - -0.2
1,180,959 - +0.1
1,180,988 - +0.3
1,180,988 - +1.2
1,180,989 - -1.1
1,180,989 - +1.0
1,181,036 - -0.3
1,181,046 - -1.1
1,181,101 + +0.5
1,181,101 + +1.5
1,181,170 - potD b1123 0.16 -0.5
1,181,280 - potD b1123 0.26 -0.3
1,181,280 - potD b1123 0.26 -0.7
1,181,421 + potD b1123 0.40 -1.5
1,181,480 - potD b1123 0.45 +0.3

Or see this region's nucleotide sequence