Experiment: LB + ciprofloxacin Time3hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1551 and cspI are separated by 345 nucleotides cspI and ydfP are separated by 362 nucleotides ydfP and rzoQ overlap by 448 nucleotides rzoQ and ydfQ are separated by 189 nucleotides ydfQ and ydfR overlap by 4 nucleotides ydfR and b1556 are separated by 4 nucleotides
b1551: b1551 - orf, hypothetical protein (VIMSS), at 1,635,978 to 1,636,133
b1551
b1552: cspI - Qin prophage; cold shock protein (NCBI), at 1,636,479 to 1,636,691
cspI
b1553: ydfP - Qin prophage; conserved protein (NCBI), at 1,637,054 to 1,637,551
ydfP
b4689: rzoQ - hypothetical protein (RefSeq), at 1,637,104 to 1,637,358
rzoQ
b1554: ydfQ - Qin prophage; predicted lysozyme (NCBI), at 1,637,548 to 1,638,081
ydfQ
b1555: ydfR - Qin prophage; predicted protein (NCBI), at 1,638,078 to 1,638,389
ydfR
b1556: b1556 - orf, hypothetical protein (VIMSS), at 1,638,394 to 1,638,609
b1556
Position (kb)
1637
1638 Strain fitness (log2 ratio)
-1
0
1
2 at 1636.078 kb on - strand, within b1551 at 1636.097 kb on - strand, within b1551 at 1636.212 kb on + strand at 1636.353 kb on - strand at 1636.354 kb on + strand at 1636.421 kb on - strand at 1636.426 kb on - strand at 1636.428 kb on - strand at 1636.434 kb on - strand at 1636.434 kb on - strand at 1636.499 kb on - strand at 1636.500 kb on - strand at 1637.122 kb on - strand at 1637.122 kb on - strand at 1637.377 kb on + strand, within ydfP at 1637.377 kb on + strand, within ydfP at 1637.385 kb on - strand, within ydfP at 1637.421 kb on - strand, within ydfP at 1637.577 kb on - strand at 1638.002 kb on + strand, within ydfQ at 1638.079 kb on + strand at 1638.405 kb on - strand at 1638.405 kb on - strand at 1638.487 kb on - strand, within b1556 at 1638.489 kb on - strand, within b1556 at 1638.489 kb on - strand, within b1556 at 1638.489 kb on - strand, within b1556 at 1638.495 kb on + strand, within b1556 at 1638.498 kb on - strand, within b1556 at 1638.513 kb on + strand, within b1556
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time3hr remove 1,636,078 - b1551 b1551 0.64 -1.2 1,636,097 - b1551 b1551 0.76 +0.8 1,636,212 + +0.8 1,636,353 - -0.2 1,636,354 + +0.8 1,636,421 - -0.8 1,636,426 - -0.1 1,636,428 - +2.4 1,636,434 - +1.4 1,636,434 - +0.8 1,636,499 - +1.8 1,636,500 - +0.8 1,637,122 - +0.8 1,637,122 - -0.3 1,637,377 + ydfP b1553 0.65 +0.2 1,637,377 + ydfP b1553 0.65 +1.6 1,637,385 - ydfP b1553 0.66 -1.3 1,637,421 - ydfP b1553 0.74 +0.8 1,637,577 - -0.3 1,638,002 + ydfQ b1554 0.85 +0.9 1,638,079 + +1.9 1,638,405 - -0.1 1,638,405 - +1.1 1,638,487 - b1556 b1556 0.43 +0.4 1,638,489 - b1556 b1556 0.44 -0.6 1,638,489 - b1556 b1556 0.44 -0.2 1,638,489 - b1556 b1556 0.44 -0.7 1,638,495 + b1556 b1556 0.47 +0.8 1,638,498 - b1556 b1556 0.48 +1.7 1,638,513 + b1556 b1556 0.55 +1.2
Or see this region's nucleotide sequence