Experiment: LB + ciprofloxacin Time3hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahM and yahN are separated by 16 nucleotides yahN and yahO are separated by 146 nucleotides yahO and prpR are separated by 97 nucleotides
b0327: yahM - orf, hypothetical protein (VIMSS), at 344,628 to 344,873
yahM
b0328: yahN - neutral amino-acid efflux system (NCBI), at 344,890 to 345,561
yahN
b0329: yahO - hypothetical protein (NCBI), at 345,708 to 345,983
yahO
b0330: prpR - DNA-binding transcriptional activator (NCBI), at 346,081 to 347,667
prpR
Position (kb) 345
346 Strain fitness (log2 ratio) -1
0
1
2 at 344.713 kb on + strand, within yahM at 344.713 kb on + strand, within yahM at 344.718 kb on + strand, within yahM at 344.718 kb on + strand, within yahM at 344.748 kb on + strand, within yahM at 344.748 kb on + strand, within yahM at 344.749 kb on + strand, within yahM at 344.751 kb on + strand, within yahM at 344.756 kb on + strand, within yahM at 344.756 kb on - strand, within yahM at 344.759 kb on - strand, within yahM at 344.828 kb on + strand, within yahM at 344.849 kb on - strand at 344.860 kb on + strand at 344.927 kb on - strand at 344.927 kb on - strand at 344.982 kb on + strand, within yahN at 344.982 kb on + strand, within yahN at 344.987 kb on + strand, within yahN at 345.056 kb on + strand, within yahN at 345.059 kb on - strand, within yahN at 345.064 kb on - strand, within yahN at 345.064 kb on - strand, within yahN at 345.065 kb on - strand, within yahN at 345.065 kb on - strand, within yahN at 345.067 kb on + strand, within yahN at 345.075 kb on - strand, within yahN at 345.078 kb on - strand, within yahN at 345.078 kb on - strand, within yahN at 345.103 kb on + strand, within yahN at 345.140 kb on + strand, within yahN at 345.146 kb on + strand, within yahN at 345.195 kb on - strand, within yahN at 345.195 kb on - strand, within yahN at 345.206 kb on + strand, within yahN at 345.210 kb on + strand, within yahN at 345.223 kb on + strand, within yahN at 345.239 kb on - strand, within yahN at 345.248 kb on + strand, within yahN at 345.248 kb on + strand, within yahN at 345.266 kb on - strand, within yahN at 345.311 kb on + strand, within yahN at 345.311 kb on + strand, within yahN at 345.378 kb on + strand, within yahN at 345.387 kb on - strand, within yahN at 345.394 kb on + strand, within yahN at 345.394 kb on + strand, within yahN at 345.402 kb on + strand, within yahN at 345.425 kb on + strand, within yahN at 345.431 kb on + strand, within yahN at 345.440 kb on + strand, within yahN at 345.441 kb on - strand, within yahN at 345.441 kb on - strand, within yahN at 345.486 kb on + strand, within yahN at 345.486 kb on + strand, within yahN at 345.486 kb on + strand, within yahN at 345.486 kb on + strand, within yahN at 345.486 kb on + strand, within yahN at 345.494 kb on - strand at 345.498 kb on + strand at 345.498 kb on + strand at 345.498 kb on + strand at 345.498 kb on + strand at 345.506 kb on - strand at 345.546 kb on + strand at 345.546 kb on + strand at 345.654 kb on - strand at 345.677 kb on - strand at 345.677 kb on - strand at 345.689 kb on - strand at 345.701 kb on - strand at 345.746 kb on - strand, within yahO at 345.750 kb on + strand, within yahO at 345.754 kb on + strand, within yahO at 345.754 kb on + strand, within yahO at 345.761 kb on + strand, within yahO at 345.761 kb on + strand, within yahO at 345.763 kb on - strand, within yahO at 345.763 kb on - strand, within yahO at 345.774 kb on + strand, within yahO at 345.834 kb on + strand, within yahO at 345.867 kb on - strand, within yahO at 345.879 kb on - strand, within yahO at 345.879 kb on - strand, within yahO at 345.918 kb on + strand, within yahO at 345.926 kb on - strand, within yahO at 345.936 kb on - strand, within yahO at 345.994 kb on + strand at 346.002 kb on + strand at 346.061 kb on - strand at 346.098 kb on + strand at 346.127 kb on + strand at 346.286 kb on - strand, within prpR at 346.286 kb on - strand, within prpR at 346.310 kb on - strand, within prpR at 346.317 kb on + strand, within prpR at 346.368 kb on + strand, within prpR at 346.406 kb on + strand, within prpR at 346.406 kb on + strand, within prpR at 346.566 kb on + strand, within prpR at 346.574 kb on + strand, within prpR at 346.574 kb on + strand, within prpR at 346.643 kb on + strand, within prpR at 346.728 kb on + strand, within prpR at 346.945 kb on - strand, within prpR
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time3hr remove 344,713 + yahM b0327 0.35 -0.3 344,713 + yahM b0327 0.35 +0.3 344,718 + yahM b0327 0.37 +0.5 344,718 + yahM b0327 0.37 -0.0 344,748 + yahM b0327 0.49 -0.5 344,748 + yahM b0327 0.49 +0.2 344,749 + yahM b0327 0.49 -0.2 344,751 + yahM b0327 0.50 +0.3 344,756 + yahM b0327 0.52 +0.5 344,756 - yahM b0327 0.52 -0.4 344,759 - yahM b0327 0.53 +0.1 344,828 + yahM b0327 0.81 +0.4 344,849 - -0.3 344,860 + -0.1 344,927 - +1.5 344,927 - +0.2 344,982 + yahN b0328 0.14 -0.3 344,982 + yahN b0328 0.14 -0.3 344,987 + yahN b0328 0.14 +0.1 345,056 + yahN b0328 0.25 +0.7 345,059 - yahN b0328 0.25 -0.2 345,064 - yahN b0328 0.26 -0.0 345,064 - yahN b0328 0.26 +0.2 345,065 - yahN b0328 0.26 +0.4 345,065 - yahN b0328 0.26 +0.3 345,067 + yahN b0328 0.26 +0.4 345,075 - yahN b0328 0.28 +0.7 345,078 - yahN b0328 0.28 +0.2 345,078 - yahN b0328 0.28 +0.2 345,103 + yahN b0328 0.32 -0.5 345,140 + yahN b0328 0.37 +0.4 345,146 + yahN b0328 0.38 -0.1 345,195 - yahN b0328 0.45 +0.1 345,195 - yahN b0328 0.45 +0.1 345,206 + yahN b0328 0.47 +0.3 345,210 + yahN b0328 0.48 +0.3 345,223 + yahN b0328 0.50 -0.3 345,239 - yahN b0328 0.52 +0.1 345,248 + yahN b0328 0.53 +0.8 345,248 + yahN b0328 0.53 -0.0 345,266 - yahN b0328 0.56 -0.6 345,311 + yahN b0328 0.63 +0.1 345,311 + yahN b0328 0.63 -0.3 345,378 + yahN b0328 0.73 -0.3 345,387 - yahN b0328 0.74 -0.2 345,394 + yahN b0328 0.75 +0.0 345,394 + yahN b0328 0.75 -1.0 345,402 + yahN b0328 0.76 +0.1 345,425 + yahN b0328 0.80 -0.4 345,431 + yahN b0328 0.81 -0.8 345,440 + yahN b0328 0.82 -0.2 345,441 - yahN b0328 0.82 -0.7 345,441 - yahN b0328 0.82 -0.5 345,486 + yahN b0328 0.89 -0.1 345,486 + yahN b0328 0.89 -0.7 345,486 + yahN b0328 0.89 -0.1 345,486 + yahN b0328 0.89 +0.1 345,486 + yahN b0328 0.89 +0.2 345,494 - -0.3 345,498 + +0.3 345,498 + +1.2 345,498 + +0.5 345,498 + -0.6 345,506 - -0.2 345,546 + +0.1 345,546 + +0.0 345,654 - +0.3 345,677 - -0.1 345,677 - +0.1 345,689 - +0.5 345,701 - -0.0 345,746 - yahO b0329 0.14 +0.3 345,750 + yahO b0329 0.15 -0.2 345,754 + yahO b0329 0.17 +1.8 345,754 + yahO b0329 0.17 +0.2 345,761 + yahO b0329 0.19 +0.1 345,761 + yahO b0329 0.19 +0.4 345,763 - yahO b0329 0.20 -0.2 345,763 - yahO b0329 0.20 +1.7 345,774 + yahO b0329 0.24 +0.1 345,834 + yahO b0329 0.46 -0.0 345,867 - yahO b0329 0.58 +0.3 345,879 - yahO b0329 0.62 +0.2 345,879 - yahO b0329 0.62 +0.3 345,918 + yahO b0329 0.76 +0.0 345,926 - yahO b0329 0.79 +0.6 345,936 - yahO b0329 0.83 +0.8 345,994 + +0.0 346,002 + +0.2 346,061 - +0.3 346,098 + +0.1 346,127 + +0.7 346,286 - prpR b0330 0.13 -0.5 346,286 - prpR b0330 0.13 +0.2 346,310 - prpR b0330 0.14 +0.0 346,317 + prpR b0330 0.15 -0.5 346,368 + prpR b0330 0.18 -0.5 346,406 + prpR b0330 0.20 +0.1 346,406 + prpR b0330 0.20 +0.6 346,566 + prpR b0330 0.31 +0.2 346,574 + prpR b0330 0.31 +0.1 346,574 + prpR b0330 0.31 +0.2 346,643 + prpR b0330 0.35 -0.4 346,728 + prpR b0330 0.41 +0.0 346,945 - prpR b0330 0.54 +0.0
Or see this region's nucleotide sequence