Experiment: No phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2021 and PP_5496 are separated by 122 nucleotides PP_5496 and PP_2023 are separated by 132 nucleotides PP_2023 and PP_2024 overlap by 10 nucleotides
PP_2021: PP_2021 - L-alpha-hydroxyglutaric acid gamma-lactonase, at 2,294,325 to 2,295,455
_2021
PP_5496: PP_5496 - conserved exported protein of unknown function, at 2,295,578 to 2,295,754
_5496
PP_2023: PP_2023 - Glutathione S-transferase family protein, at 2,295,887 to 2,296,486
_2023
PP_2024: PP_2024 - Exonuclease SbcC, at 2,296,477 to 2,300,121
_2024
Position (kb)
2295
2296 Strain fitness (log2 ratio)
-1
0
1
2 at 2294.685 kb on + strand, within PP_2021 at 2294.685 kb on + strand, within PP_2021 at 2294.704 kb on - strand, within PP_2021 at 2294.704 kb on - strand, within PP_2021 at 2294.796 kb on - strand, within PP_2021 at 2294.877 kb on + strand, within PP_2021 at 2294.878 kb on - strand, within PP_2021 at 2294.913 kb on + strand, within PP_2021 at 2294.913 kb on + strand, within PP_2021 at 2294.914 kb on - strand, within PP_2021 at 2294.914 kb on - strand, within PP_2021 at 2295.034 kb on - strand, within PP_2021 at 2295.186 kb on + strand, within PP_2021 at 2295.186 kb on + strand, within PP_2021 at 2295.186 kb on + strand, within PP_2021 at 2295.187 kb on - strand, within PP_2021 at 2295.367 kb on + strand at 2295.367 kb on + strand at 2295.367 kb on + strand at 2295.368 kb on - strand at 2295.368 kb on - strand at 2295.368 kb on - strand at 2295.472 kb on - strand at 2295.478 kb on + strand at 2295.570 kb on - strand at 2295.602 kb on - strand, within PP_5496 at 2295.686 kb on + strand, within PP_5496 at 2295.686 kb on + strand, within PP_5496 at 2295.687 kb on - strand, within PP_5496 at 2295.699 kb on - strand, within PP_5496 at 2296.057 kb on - strand, within PP_2023 at 2296.158 kb on + strand at 2296.158 kb on + strand, within PP_2023 at 2296.158 kb on + strand, within PP_2023 at 2296.158 kb on + strand, within PP_2023 at 2296.242 kb on - strand, within PP_2023 at 2296.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No phage control remove 2,294,685 + PP_2021 0.32 -0.6 2,294,685 + PP_2021 0.32 +0.2 2,294,704 - PP_2021 0.34 +0.5 2,294,704 - PP_2021 0.34 -0.4 2,294,796 - PP_2021 0.42 -0.5 2,294,877 + PP_2021 0.49 +0.3 2,294,878 - PP_2021 0.49 -0.0 2,294,913 + PP_2021 0.52 +0.8 2,294,913 + PP_2021 0.52 +0.5 2,294,914 - PP_2021 0.52 +0.0 2,294,914 - PP_2021 0.52 -0.5 2,295,034 - PP_2021 0.63 +2.6 2,295,186 + PP_2021 0.76 -0.5 2,295,186 + PP_2021 0.76 -1.0 2,295,186 + PP_2021 0.76 -1.7 2,295,187 - PP_2021 0.76 -0.1 2,295,367 + +1.2 2,295,367 + +0.8 2,295,367 + -1.5 2,295,368 - +0.5 2,295,368 - +1.4 2,295,368 - -0.1 2,295,472 - -1.4 2,295,478 + -0.5 2,295,570 - -0.2 2,295,602 - PP_5496 0.14 -0.5 2,295,686 + PP_5496 0.61 +0.4 2,295,686 + PP_5496 0.61 +0.2 2,295,687 - PP_5496 0.62 +1.6 2,295,699 - PP_5496 0.68 +0.3 2,296,057 - PP_2023 0.28 +0.3 2,296,158 + +0.8 2,296,158 + PP_2023 0.45 -0.5 2,296,158 + PP_2023 0.45 -0.1 2,296,158 + PP_2023 0.45 -0.7 2,296,242 - PP_2023 0.59 +1.2 2,296,562 - +0.4
Or see this region's nucleotide sequence