Strain Fitness in Escherichia coli BW25113 around b3074

Experiment: LB + carbenicillin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygjG and ygjH are separated by 41 nucleotidesygjH and ebgR are separated by 218 nucleotides b3073: ygjG - putrescine:2-oxoglutaric acid aminotransferase, PLP-dependent (RefSeq), at 3,217,516 to 3,218,895 ygjG b3074: ygjH - hypothetical protein (NCBI), at 3,218,937 to 3,219,269 ygjH b3075: ebgR - DNA-binding transcriptional repressor (NCBI), at 3,219,488 to 3,220,471 ebgR Position (kb) 3218 3219 3220Strain fitness (log2 ratio) -2 -1 0 1at 3218.060 kb on + strand, within ygjGat 3218.112 kb on + strand, within ygjGat 3218.112 kb on + strand, within ygjGat 3218.118 kb on - strand, within ygjGat 3218.222 kb on + strand, within ygjGat 3218.231 kb on - strand, within ygjGat 3218.276 kb on - strand, within ygjGat 3218.293 kb on - strand, within ygjGat 3218.293 kb on - strand, within ygjGat 3218.338 kb on - strand, within ygjGat 3218.356 kb on + strand, within ygjGat 3218.387 kb on - strand, within ygjGat 3218.407 kb on - strand, within ygjGat 3218.407 kb on - strand, within ygjGat 3218.494 kb on + strand, within ygjGat 3218.532 kb on - strand, within ygjGat 3218.591 kb on - strand, within ygjGat 3218.591 kb on - strand, within ygjGat 3218.663 kb on - strand, within ygjGat 3218.663 kb on - strand, within ygjGat 3218.826 kb on + strandat 3218.833 kb on - strandat 3218.855 kb on + strandat 3218.959 kb on + strandat 3218.992 kb on + strand, within ygjHat 3218.992 kb on + strand, within ygjHat 3219.018 kb on + strand, within ygjHat 3219.018 kb on + strand, within ygjHat 3219.077 kb on - strand, within ygjHat 3219.077 kb on - strand, within ygjHat 3219.077 kb on - strand, within ygjHat 3219.117 kb on - strand, within ygjHat 3219.119 kb on + strand, within ygjHat 3219.384 kb on - strandat 3219.384 kb on - strandat 3219.431 kb on - strandat 3219.462 kb on + strandat 3219.549 kb on - strandat 3219.549 kb on - strandat 3219.628 kb on - strand, within ebgRat 3219.628 kb on - strand, within ebgRat 3219.650 kb on - strand, within ebgRat 3219.689 kb on + strand, within ebgRat 3219.691 kb on + strand, within ebgRat 3219.787 kb on + strand, within ebgRat 3219.803 kb on - strand, within ebgRat 3219.818 kb on - strand, within ebgRat 3219.861 kb on + strand, within ebgRat 3220.087 kb on - strand, within ebgRat 3220.192 kb on - strand, within ebgRat 3220.237 kb on - strand, within ebgRat 3220.237 kb on - strand, within ebgR

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr
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3,218,060 + ygjG b3073 0.39 -0.5
3,218,112 + ygjG b3073 0.43 -0.1
3,218,112 + ygjG b3073 0.43 +0.0
3,218,118 - ygjG b3073 0.44 +0.2
3,218,222 + ygjG b3073 0.51 +0.0
3,218,231 - ygjG b3073 0.52 +0.3
3,218,276 - ygjG b3073 0.55 -0.3
3,218,293 - ygjG b3073 0.56 +0.3
3,218,293 - ygjG b3073 0.56 +0.2
3,218,338 - ygjG b3073 0.60 -0.4
3,218,356 + ygjG b3073 0.61 -0.3
3,218,387 - ygjG b3073 0.63 +0.2
3,218,407 - ygjG b3073 0.65 +0.6
3,218,407 - ygjG b3073 0.65 -1.2
3,218,494 + ygjG b3073 0.71 -0.3
3,218,532 - ygjG b3073 0.74 -0.4
3,218,591 - ygjG b3073 0.78 -0.4
3,218,591 - ygjG b3073 0.78 -0.0
3,218,663 - ygjG b3073 0.83 -0.2
3,218,663 - ygjG b3073 0.83 -1.1
3,218,826 + -0.8
3,218,833 - -0.4
3,218,855 + +0.2
3,218,959 + -0.7
3,218,992 + ygjH b3074 0.17 +0.3
3,218,992 + ygjH b3074 0.17 +0.3
3,219,018 + ygjH b3074 0.24 +0.5
3,219,018 + ygjH b3074 0.24 -0.2
3,219,077 - ygjH b3074 0.42 +0.3
3,219,077 - ygjH b3074 0.42 -0.1
3,219,077 - ygjH b3074 0.42 +0.0
3,219,117 - ygjH b3074 0.54 +0.0
3,219,119 + ygjH b3074 0.55 +0.5
3,219,384 - -1.1
3,219,384 - -0.3
3,219,431 - -0.2
3,219,462 + +0.2
3,219,549 - -0.7
3,219,549 - -0.4
3,219,628 - ebgR b3075 0.14 +0.3
3,219,628 - ebgR b3075 0.14 -0.5
3,219,650 - ebgR b3075 0.16 -0.5
3,219,689 + ebgR b3075 0.20 +0.5
3,219,691 + ebgR b3075 0.21 +0.4
3,219,787 + ebgR b3075 0.30 +0.0
3,219,803 - ebgR b3075 0.32 -0.2
3,219,818 - ebgR b3075 0.34 -0.6
3,219,861 + ebgR b3075 0.38 +0.7
3,220,087 - ebgR b3075 0.61 -0.2
3,220,192 - ebgR b3075 0.72 -1.2
3,220,237 - ebgR b3075 0.76 -0.6
3,220,237 - ebgR b3075 0.76 -2.4

Or see this region's nucleotide sequence