Experiment: LB + carbenicillin Time1-2hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt araF and yecI are separated by 796 nucleotides yecI and b1903 are separated by 78 nucleotides b1903 and azuC are separated by 114 nucleotides azuC and yecR are separated by 262 nucleotides
b1901: araF - L-arabinose transporter subunit (NCBI), at 1,983,163 to 1,984,152
araF
b1902: yecI - predicted ferritin-like protein (NCBI), at 1,984,949 to 1,985,452
yecI
b4537: b1903 - orf, hypothetical protein (VIMSS), at 1,985,531 to 1,985,782
b1903
b4663: azuC - expressed protein (RefSeq), at 1,985,897 to 1,985,983
azuC
b1904: yecR - hypothetical protein (NCBI), at 1,986,246 to 1,986,569
yecR
Position (kb)
1984
1985
1986 Strain fitness (log2 ratio)
-1
0
1
2 at 1984.117 kb on + strand at 1984.167 kb on - strand at 1984.167 kb on - strand at 1984.167 kb on - strand at 1984.167 kb on - strand at 1984.167 kb on - strand at 1984.180 kb on + strand at 1984.300 kb on + strand at 1984.305 kb on - strand at 1984.313 kb on - strand at 1984.604 kb on + strand at 1984.838 kb on - strand at 1984.851 kb on + strand at 1984.917 kb on - strand at 1984.917 kb on - strand at 1984.925 kb on - strand at 1984.938 kb on - strand at 1984.938 kb on - strand at 1984.938 kb on - strand at 1984.964 kb on + strand at 1985.012 kb on + strand, within yecI at 1985.015 kb on - strand, within yecI at 1985.015 kb on - strand, within yecI at 1985.019 kb on + strand, within yecI at 1985.056 kb on + strand, within yecI at 1985.059 kb on + strand, within yecI at 1985.059 kb on + strand, within yecI at 1985.092 kb on + strand, within yecI at 1985.100 kb on - strand, within yecI at 1985.263 kb on - strand, within yecI at 1985.263 kb on - strand, within yecI at 1985.330 kb on + strand, within yecI at 1985.369 kb on - strand, within yecI at 1985.369 kb on - strand, within yecI at 1985.410 kb on - strand at 1985.410 kb on - strand at 1985.428 kb on - strand at 1985.449 kb on + strand at 1985.449 kb on + strand at 1985.463 kb on + strand at 1985.821 kb on - strand at 1985.843 kb on + strand at 1985.843 kb on + strand at 1985.843 kb on + strand at 1985.935 kb on + strand, within azuC at 1986.136 kb on - strand at 1986.150 kb on - strand at 1986.175 kb on - strand at 1986.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr remove 1,984,117 + -0.6 1,984,167 - -0.7 1,984,167 - -0.1 1,984,167 - +0.7 1,984,167 - +0.7 1,984,167 - +0.2 1,984,180 + +0.1 1,984,300 + +0.2 1,984,305 - +0.2 1,984,313 - -0.5 1,984,604 + +0.8 1,984,838 - +0.2 1,984,851 + +0.2 1,984,917 - +0.8 1,984,917 - -0.1 1,984,925 - +0.1 1,984,938 - +0.2 1,984,938 - +1.5 1,984,938 - +1.1 1,984,964 + -0.5 1,985,012 + yecI b1902 0.12 +1.8 1,985,015 - yecI b1902 0.13 +0.3 1,985,015 - yecI b1902 0.13 +1.2 1,985,019 + yecI b1902 0.14 +0.8 1,985,056 + yecI b1902 0.21 -0.0 1,985,059 + yecI b1902 0.22 +0.5 1,985,059 + yecI b1902 0.22 +0.6 1,985,092 + yecI b1902 0.28 -0.3 1,985,100 - yecI b1902 0.30 +0.3 1,985,263 - yecI b1902 0.62 +1.5 1,985,263 - yecI b1902 0.62 -0.7 1,985,330 + yecI b1902 0.76 -0.2 1,985,369 - yecI b1902 0.83 -0.4 1,985,369 - yecI b1902 0.83 +0.8 1,985,410 - -0.2 1,985,410 - +0.2 1,985,428 - -0.3 1,985,449 + -0.9 1,985,449 + +0.5 1,985,463 + -0.1 1,985,821 - +1.2 1,985,843 + -0.3 1,985,843 + -1.3 1,985,843 + -0.4 1,985,935 + azuC b4663 0.44 -0.1 1,986,136 - -1.2 1,986,150 - +0.2 1,986,175 - -0.2 1,986,232 - +0.1
Or see this region's nucleotide sequence