Strain Fitness in Escherichia coli BW25113 around b1730

Experiment: LB + carbenicillin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydjN and ydjO are separated by 3 nucleotidesydjO and cedA are separated by 288 nucleotidescedA and katE are separated by 203 nucleotides b1729: ydjN - predicted transporter (NCBI), at 1,808,958 to 1,810,349 ydjN b1730: ydjO - predicted protein (RefSeq), at 1,810,353 to 1,811,156 ydjO b1731: cedA - cell division modulator (RefSeq), at 1,811,445 to 1,811,687 cedA b1732: katE - hydroperoxidase HPII(III) (catalase) (NCBI), at 1,811,891 to 1,814,152 katE Position (kb) 1810 1811 1812Strain fitness (log2 ratio) -1 0 1 2at 1809.720 kb on + strand, within ydjNat 1809.720 kb on + strand, within ydjNat 1810.253 kb on + strandat 1810.253 kb on + strandat 1810.284 kb on + strandat 1810.284 kb on + strandat 1810.343 kb on + strandat 1810.445 kb on - strand, within ydjOat 1810.493 kb on + strand, within ydjOat 1810.515 kb on + strand, within ydjOat 1811.304 kb on - strandat 1811.393 kb on - strandat 1811.444 kb on - strandat 1811.505 kb on - strand, within cedAat 1811.536 kb on - strand, within cedAat 1811.536 kb on - strand, within cedAat 1811.541 kb on + strand, within cedAat 1811.814 kb on + strandat 1811.821 kb on + strandat 1812.027 kb on + strandat 1812.052 kb on + strandat 1812.060 kb on - strandat 1812.106 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr
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1,809,720 + ydjN b1729 0.55 +0.1
1,809,720 + ydjN b1729 0.55 +1.3
1,810,253 + -0.9
1,810,253 + -0.6
1,810,284 + -0.1
1,810,284 + -0.3
1,810,343 + -0.3
1,810,445 - ydjO b1730 0.11 +2.0
1,810,493 + ydjO b1730 0.17 +0.0
1,810,515 + ydjO b1730 0.20 +2.0
1,811,304 - +2.0
1,811,393 - +1.0
1,811,444 - +0.0
1,811,505 - cedA b1731 0.25 -0.4
1,811,536 - cedA b1731 0.37 +0.2
1,811,536 - cedA b1731 0.37 +0.2
1,811,541 + cedA b1731 0.40 +0.0
1,811,814 + -0.1
1,811,821 + -0.3
1,812,027 + +1.0
1,812,052 + -0.3
1,812,060 - -0.5
1,812,106 - -0.2

Or see this region's nucleotide sequence