Strain Fitness in Escherichia coli BW25113 around b1690

Experiment: LB + carbenicillin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiK and rprA are separated by 185 nucleotidesrprA and b1689 are separated by 137 nucleotidesb1689 and ydiM are separated by 99 nucleotidesydiM and ydiN are separated by 226 nucleotides b1688: ydiK - predicted inner membrane protein (NCBI), at 1,767,098 to 1,768,210 ydiK b4431: rprA - Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq), at 1,768,396 to 1,768,501 rprA b1689: b1689 - orf, hypothetical protein (VIMSS), at 1,768,639 to 1,768,995 b1689 b1690: ydiM - predicted transporter (NCBI), at 1,769,095 to 1,770,309 ydiM b1691: ydiN - predicted transporter (RefSeq), at 1,770,536 to 1,771,801 ydiN Position (kb) 1769 1770 1771Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1768.225 kb on + strandat 1768.225 kb on + strandat 1768.227 kb on + strandat 1768.290 kb on - strandat 1768.307 kb on + strandat 1768.307 kb on + strandat 1768.312 kb on - strandat 1768.382 kb on + strandat 1768.390 kb on - strandat 1768.466 kb on - strand, within rprAat 1768.471 kb on - strand, within rprAat 1768.479 kb on + strand, within rprAat 1768.516 kb on + strandat 1768.551 kb on + strandat 1768.559 kb on - strandat 1768.561 kb on + strandat 1768.561 kb on + strandat 1768.569 kb on + strandat 1768.569 kb on + strandat 1768.569 kb on - strandat 1768.571 kb on + strandat 1768.654 kb on + strandat 1768.661 kb on - strandat 1768.685 kb on + strand, within b1689at 1768.714 kb on - strand, within b1689at 1768.720 kb on + strand, within b1689at 1768.765 kb on + strand, within b1689at 1768.765 kb on + strand, within b1689at 1768.781 kb on - strand, within b1689at 1768.788 kb on + strand, within b1689at 1768.788 kb on + strand, within b1689at 1768.905 kb on + strand, within b1689at 1768.905 kb on + strand, within b1689at 1768.905 kb on + strand, within b1689at 1768.913 kb on - strand, within b1689at 1768.976 kb on + strandat 1769.159 kb on - strandat 1769.186 kb on - strandat 1769.198 kb on + strandat 1769.223 kb on + strand, within ydiMat 1769.230 kb on + strand, within ydiMat 1769.238 kb on - strand, within ydiMat 1769.244 kb on + strand, within ydiMat 1769.244 kb on + strand, within ydiMat 1769.323 kb on + strand, within ydiMat 1769.331 kb on - strand, within ydiMat 1769.341 kb on - strand, within ydiMat 1769.401 kb on + strand, within ydiMat 1769.461 kb on + strand, within ydiMat 1769.464 kb on + strand, within ydiMat 1769.548 kb on - strand, within ydiMat 1769.556 kb on - strand, within ydiMat 1769.561 kb on - strand, within ydiMat 1769.566 kb on - strand, within ydiMat 1769.641 kb on - strand, within ydiMat 1769.646 kb on + strand, within ydiMat 1769.653 kb on - strand, within ydiMat 1769.751 kb on - strand, within ydiMat 1769.770 kb on - strand, within ydiMat 1769.791 kb on - strand, within ydiMat 1769.817 kb on + strand, within ydiMat 1769.817 kb on + strand, within ydiMat 1769.817 kb on - strand, within ydiMat 1769.847 kb on - strand, within ydiMat 1769.847 kb on - strand, within ydiMat 1769.942 kb on - strand, within ydiMat 1769.942 kb on - strand, within ydiMat 1769.951 kb on + strand, within ydiMat 1770.096 kb on + strand, within ydiMat 1770.106 kb on + strand, within ydiMat 1770.858 kb on - strand, within ydiNat 1770.860 kb on - strand, within ydiNat 1770.860 kb on - strand, within ydiNat 1770.870 kb on - strand, within ydiNat 1770.870 kb on - strand, within ydiNat 1770.870 kb on - strand, within ydiNat 1770.882 kb on - strand, within ydiNat 1770.906 kb on - strand, within ydiNat 1771.124 kb on + strand, within ydiNat 1771.160 kb on + strand, within ydiNat 1771.195 kb on + strand, within ydiNat 1771.269 kb on + strand, within ydiNat 1771.273 kb on + strand, within ydiNat 1771.273 kb on + strand, within ydiNat 1771.273 kb on + strand, within ydiN

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr
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1,768,225 + +1.6
1,768,225 + +0.5
1,768,227 + +0.6
1,768,290 - -0.1
1,768,307 + -0.5
1,768,307 + +0.6
1,768,312 - +0.2
1,768,382 + -0.4
1,768,390 - -0.2
1,768,466 - rprA b4431 0.66 +0.5
1,768,471 - rprA b4431 0.71 +0.1
1,768,479 + rprA b4431 0.78 +0.1
1,768,516 + +2.1
1,768,551 + +0.0
1,768,559 - -0.0
1,768,561 + +0.1
1,768,561 + +0.3
1,768,569 + -0.6
1,768,569 + -0.4
1,768,569 - -2.5
1,768,571 + -1.4
1,768,654 + +2.1
1,768,661 - +0.6
1,768,685 + b1689 b1689 0.13 +1.1
1,768,714 - b1689 b1689 0.21 -0.7
1,768,720 + b1689 b1689 0.23 -0.5
1,768,765 + b1689 b1689 0.35 +0.1
1,768,765 + b1689 b1689 0.35 -0.6
1,768,781 - b1689 b1689 0.40 +2.1
1,768,788 + b1689 b1689 0.42 +0.3
1,768,788 + b1689 b1689 0.42 +0.4
1,768,905 + b1689 b1689 0.75 +1.1
1,768,905 + b1689 b1689 0.75 +0.6
1,768,905 + b1689 b1689 0.75 +1.3
1,768,913 - b1689 b1689 0.77 +2.1
1,768,976 + -0.3
1,769,159 - +1.8
1,769,186 - +2.1
1,769,198 + +1.1
1,769,223 + ydiM b1690 0.11 -1.0
1,769,230 + ydiM b1690 0.11 +2.1
1,769,238 - ydiM b1690 0.12 +2.1
1,769,244 + ydiM b1690 0.12 +0.6
1,769,244 + ydiM b1690 0.12 +2.1
1,769,323 + ydiM b1690 0.19 +2.1
1,769,331 - ydiM b1690 0.19 +2.1
1,769,341 - ydiM b1690 0.20 +2.1
1,769,401 + ydiM b1690 0.25 +3.1
1,769,461 + ydiM b1690 0.30 -0.3
1,769,464 + ydiM b1690 0.30 +0.7
1,769,548 - ydiM b1690 0.37 -0.8
1,769,556 - ydiM b1690 0.38 +0.5
1,769,561 - ydiM b1690 0.38 -0.5
1,769,566 - ydiM b1690 0.39 +1.1
1,769,641 - ydiM b1690 0.45 +3.1
1,769,646 + ydiM b1690 0.45 +2.1
1,769,653 - ydiM b1690 0.46 -0.8
1,769,751 - ydiM b1690 0.54 +2.1
1,769,770 - ydiM b1690 0.56 +2.1
1,769,791 - ydiM b1690 0.57 +2.1
1,769,817 + ydiM b1690 0.59 +2.1
1,769,817 + ydiM b1690 0.59 +2.1
1,769,817 - ydiM b1690 0.59 +2.1
1,769,847 - ydiM b1690 0.62 +2.1
1,769,847 - ydiM b1690 0.62 +2.1
1,769,942 - ydiM b1690 0.70 +2.1
1,769,942 - ydiM b1690 0.70 +2.1
1,769,951 + ydiM b1690 0.70 +0.1
1,770,096 + ydiM b1690 0.82 +2.1
1,770,106 + ydiM b1690 0.83 +2.1
1,770,858 - ydiN b1691 0.25 +1.1
1,770,860 - ydiN b1691 0.26 +2.1
1,770,860 - ydiN b1691 0.26 +2.1
1,770,870 - ydiN b1691 0.26 +0.5
1,770,870 - ydiN b1691 0.26 +0.6
1,770,870 - ydiN b1691 0.26 +1.1
1,770,882 - ydiN b1691 0.27 +2.1
1,770,906 - ydiN b1691 0.29 +2.1
1,771,124 + ydiN b1691 0.46 +3.1
1,771,160 + ydiN b1691 0.49 +2.1
1,771,195 + ydiN b1691 0.52 +0.9
1,771,269 + ydiN b1691 0.58 +2.1
1,771,273 + ydiN b1691 0.58 +0.7
1,771,273 + ydiN b1691 0.58 -0.2
1,771,273 + ydiN b1691 0.58 +0.7

Or see this region's nucleotide sequence