Strain Fitness in Escherichia coli BW25113 around b1545

Experiment: LB + carbenicillin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydfK and pinQ are separated by 316 nucleotidespinQ and tfaQ are separated by 97 nucleotidestfaQ and stfQ overlap by 1 nucleotides b1544: ydfK - cold shock protein, function unknown, Qin prophage (NCBI), at 1,631,096 to 1,631,329 ydfK b1545: pinQ - Qin prophage; predicted site-specific recombinase (NCBI), at 1,631,646 to 1,632,236 pinQ b1546: tfaQ - Qin prophage; predicted tail fibre assembly protein (NCBI), at 1,632,334 to 1,632,909 tfaQ b1547: stfQ - Qin prophage; predicted side tail fibre assembly protein (NCBI), at 1,632,909 to 1,633,871 stfQ Position (kb) 1631 1632 1633Strain fitness (log2 ratio) -2 -1 0 1 2at 1632.142 kb on - strand, within pinQat 1632.995 kb on + strandat 1633.092 kb on - strand, within stfQat 1633.234 kb on + strand, within stfQat 1633.234 kb on + strand, within stfQ

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr
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1,632,142 - pinQ b1545 0.84 +2.1
1,632,995 + -2.1
1,633,092 - stfQ b1547 0.19 -0.2
1,633,234 + stfQ b1547 0.34 -0.8
1,633,234 + stfQ b1547 0.34 +0.3

Or see this region's nucleotide sequence