Experiment: LB + carbenicillin Time1-2hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recE and racC are separated by 101 nucleotides racC and ydaE are separated by 74 nucleotides ydaE and ydaD overlap by 1 nucleotides ydaD and sieB are separated by 441 nucleotides
b1350: recE - Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease (NCBI), at 1,412,810 to 1,415,410
recE
b1351: racC - Rac prophage; predicted protein (NCBI), at 1,415,512 to 1,415,787
racC
b4526: ydaE - Rac prophage; conserved protein (NCBI), at 1,415,862 to 1,416,032
ydaE
b1352: ydaD - orf, hypothetical protein (VIMSS), at 1,416,032 to 1,416,253
ydaD
b1353: sieB - phage superinfection exclusion protein (VIMSS), at 1,416,695 to 1,417,183
sieB
Position (kb)
1415
1416 Strain fitness (log2 ratio)
-1
0
1
2 at 1414.602 kb on - strand, within recE at 1414.633 kb on - strand, within recE at 1414.633 kb on - strand, within recE at 1414.719 kb on - strand, within recE at 1414.747 kb on + strand, within recE at 1414.758 kb on + strand, within recE at 1414.758 kb on + strand, within recE at 1415.046 kb on + strand, within recE at 1415.106 kb on + strand, within recE at 1415.187 kb on + strand at 1415.187 kb on + strand at 1415.195 kb on - strand at 1415.195 kb on - strand at 1415.195 kb on - strand at 1415.300 kb on + strand at 1415.416 kb on - strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.484 kb on + strand at 1415.538 kb on + strand at 1415.620 kb on + strand, within racC at 1415.625 kb on - strand, within racC at 1415.649 kb on - strand, within racC at 1416.473 kb on + strand at 1416.624 kb on - strand at 1416.644 kb on - strand at 1416.679 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr remove 1,414,602 - recE b1350 0.69 -0.0 1,414,633 - recE b1350 0.70 -0.6 1,414,633 - recE b1350 0.70 +0.7 1,414,719 - recE b1350 0.73 -0.4 1,414,747 + recE b1350 0.74 -0.3 1,414,758 + recE b1350 0.75 +0.5 1,414,758 + recE b1350 0.75 +0.2 1,415,046 + recE b1350 0.86 -0.0 1,415,106 + recE b1350 0.88 +0.0 1,415,187 + +0.4 1,415,187 + -0.0 1,415,195 - -0.2 1,415,195 - -0.3 1,415,195 - +0.4 1,415,300 + -0.2 1,415,416 - +2.0 1,415,468 + +0.1 1,415,468 + -0.8 1,415,468 + +0.7 1,415,468 + -0.1 1,415,468 + -0.3 1,415,468 + -0.0 1,415,484 + -0.2 1,415,538 + +2.0 1,415,620 + racC b1351 0.39 +2.0 1,415,625 - racC b1351 0.41 +2.0 1,415,649 - racC b1351 0.50 +2.0 1,416,473 + +2.0 1,416,624 - +2.0 1,416,644 - -0.6 1,416,679 - +2.0
Or see this region's nucleotide sequence