Experiment: P. putida Sci Big MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0405 and PP_0406 overlap by 4 nucleotides PP_0406 and PP_0407 are separated by 1 nucleotides PP_0407 and PP_0408 are separated by 27 nucleotides
PP_0405: PP_0405 - conserved protein of unknown function, at 492,551 to 493,570
_0405
PP_0406: PP_0406 - Nucleotidyltransferase family protein, at 493,567 to 494,238
_0406
PP_0407: PP_0407 - DnaJ-like protein DjlA, at 494,240 to 495,007
_0407
PP_0408: PP_0408 - conserved exported protein of unknown function, at 495,035 to 496,033
_0408
Position (kb) 494
495
496 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.241 kb on - strand, within PP_0405 at 493.241 kb on - strand, within PP_0405 at 493.454 kb on + strand, within PP_0405 at 493.455 kb on - strand, within PP_0405 at 493.484 kb on + strand at 493.485 kb on - strand at 493.485 kb on - strand at 493.486 kb on + strand at 493.487 kb on - strand at 493.487 kb on - strand at 493.488 kb on + strand at 493.488 kb on + strand at 493.488 kb on + strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.673 kb on - strand, within PP_0406 at 493.901 kb on - strand, within PP_0406 at 494.147 kb on + strand, within PP_0406 at 494.148 kb on - strand, within PP_0406 at 494.161 kb on + strand, within PP_0406 at 494.161 kb on + strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.473 kb on + strand, within PP_0407 at 494.473 kb on + strand, within PP_0407 at 494.626 kb on + strand, within PP_0407 at 494.801 kb on + strand, within PP_0407 at 494.802 kb on - strand, within PP_0407 at 494.802 kb on - strand, within PP_0407 at 494.868 kb on - strand, within PP_0407 at 494.912 kb on - strand, within PP_0407 at 494.912 kb on - strand, within PP_0407 at 494.975 kb on + strand at 494.975 kb on + strand at 494.976 kb on - strand at 494.976 kb on - strand at 495.139 kb on + strand, within PP_0408 at 495.139 kb on + strand, within PP_0408 at 495.207 kb on + strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.208 kb on - strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.210 kb on + strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.211 kb on - strand, within PP_0408 at 495.214 kb on + strand, within PP_0408 at 495.214 kb on + strand, within PP_0408 at 495.308 kb on + strand, within PP_0408 at 495.336 kb on + strand, within PP_0408 at 495.337 kb on - strand, within PP_0408 at 495.351 kb on + strand, within PP_0408 at 495.361 kb on + strand, within PP_0408 at 495.362 kb on - strand, within PP_0408 at 495.378 kb on - strand, within PP_0408
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Sci Big MOI 0.1 remove 493,240 + PP_0405 0.68 +2.5 493,240 + PP_0405 0.68 -2.2 493,240 + PP_0405 0.68 -1.2 493,240 + PP_0405 0.68 +1.7 493,240 + PP_0405 0.68 +1.8 493,240 + PP_0405 0.68 +2.1 493,241 - PP_0405 0.68 +1.9 493,241 - PP_0405 0.68 +1.5 493,454 + PP_0405 0.89 +2.7 493,455 - PP_0405 0.89 +3.0 493,484 + +2.1 493,485 - +2.3 493,485 - +1.7 493,486 + +0.9 493,487 - -0.3 493,487 - +1.0 493,488 + +1.1 493,488 + -0.1 493,488 + +2.7 493,489 - +2.1 493,489 - +1.0 493,489 - +1.9 493,489 - +1.5 493,489 - +0.2 493,489 - +1.9 493,673 - PP_0406 0.16 +0.3 493,901 - PP_0406 0.50 +1.1 494,147 + PP_0406 0.86 +1.1 494,148 - PP_0406 0.86 +2.5 494,161 + PP_0406 0.88 +0.3 494,161 + PP_0406 0.88 +2.9 494,162 - PP_0406 0.89 +0.1 494,162 - PP_0406 0.89 +1.1 494,162 - PP_0406 0.89 +2.2 494,473 + PP_0407 0.30 -0.6 494,473 + PP_0407 0.30 -0.6 494,626 + PP_0407 0.50 +0.2 494,801 + PP_0407 0.73 -2.2 494,802 - PP_0407 0.73 -2.6 494,802 - PP_0407 0.73 -2.2 494,868 - PP_0407 0.82 -1.1 494,912 - PP_0407 0.88 -2.3 494,912 - PP_0407 0.88 -0.8 494,975 + +1.4 494,975 + -2.1 494,976 - -0.1 494,976 - +1.6 495,139 + PP_0408 0.10 +0.0 495,139 + PP_0408 0.10 +1.0 495,207 + PP_0408 0.17 -2.0 495,208 - PP_0408 0.17 -0.1 495,208 - PP_0408 0.17 +0.3 495,208 - PP_0408 0.17 +0.4 495,208 - PP_0408 0.17 +0.1 495,210 + PP_0408 0.18 +0.9 495,210 + PP_0408 0.18 +0.8 495,210 + PP_0408 0.18 -1.6 495,210 + PP_0408 0.18 +1.3 495,210 + PP_0408 0.18 -0.8 495,210 + PP_0408 0.18 +0.4 495,210 + PP_0408 0.18 -1.7 495,210 + PP_0408 0.18 -0.8 495,211 - PP_0408 0.18 +0.0 495,211 - PP_0408 0.18 -0.7 495,211 - PP_0408 0.18 +1.0 495,211 - PP_0408 0.18 -1.3 495,211 - PP_0408 0.18 +1.3 495,214 + PP_0408 0.18 +0.6 495,214 + PP_0408 0.18 +0.9 495,308 + PP_0408 0.27 -0.6 495,336 + PP_0408 0.30 +0.8 495,337 - PP_0408 0.30 +1.0 495,351 + PP_0408 0.32 -1.6 495,361 + PP_0408 0.33 -0.4 495,362 - PP_0408 0.33 -0.6 495,378 - PP_0408 0.34 +1.5
Or see this region's nucleotide sequence