Experiment: P. putida PUT10 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1811 and PP_1812 are separated by 121 nucleotides PP_1812 and PP_1813 are separated by 97 nucleotides PP_1813 and PP_1814 are separated by 36 nucleotides PP_1814 and PP_1815 are separated by 183 nucleotides
PP_1811: PP_1811 - UDP-N-acetylglucosamine-2-epimerase, at 2,038,337 to 2,039,479
_1811
PP_1812: PP_1812 - conserved protein of unknown function, at 2,039,601 to 2,039,801
_1812
PP_1813: PP_1813 - ComEA-related protein, at 2,039,899 to 2,040,237
_1813
PP_1814: PP_1814 - conserved protein of unknown function, at 2,040,274 to 2,040,441
_1814
PP_1815: PP_1815 - Orotidine 5'-phosphate decarboxylase, at 2,040,625 to 2,041,326
_1815
Position (kb)
2039
2040
2041 Strain fitness (log2 ratio)
-2
-1
0
1 at 2039.097 kb on + strand, within PP_1811 at 2039.097 kb on + strand at 2039.098 kb on - strand, within PP_1811 at 2039.098 kb on - strand, within PP_1811 at 2039.098 kb on - strand, within PP_1811 at 2039.126 kb on + strand, within PP_1811 at 2039.126 kb on + strand, within PP_1811 at 2039.127 kb on - strand, within PP_1811 at 2039.151 kb on + strand, within PP_1811 at 2039.151 kb on + strand, within PP_1811 at 2039.154 kb on + strand, within PP_1811 at 2039.154 kb on + strand, within PP_1811 at 2039.155 kb on - strand, within PP_1811 at 2039.155 kb on - strand, within PP_1811 at 2039.155 kb on - strand, within PP_1811 at 2039.155 kb on - strand, within PP_1811 at 2039.307 kb on + strand, within PP_1811 at 2039.336 kb on + strand, within PP_1811 at 2039.581 kb on - strand at 2039.632 kb on + strand, within PP_1812 at 2039.688 kb on + strand, within PP_1812 at 2039.689 kb on - strand, within PP_1812 at 2039.811 kb on + strand at 2039.820 kb on - strand at 2039.917 kb on - strand at 2040.072 kb on + strand, within PP_1813 at 2040.120 kb on + strand, within PP_1813 at 2040.121 kb on - strand, within PP_1813 at 2040.189 kb on - strand, within PP_1813 at 2040.235 kb on + strand at 2040.235 kb on + strand at 2040.236 kb on - strand at 2040.313 kb on - strand, within PP_1814 at 2040.313 kb on - strand, within PP_1814 at 2040.322 kb on + strand, within PP_1814 at 2040.322 kb on + strand, within PP_1814 at 2040.322 kb on + strand, within PP_1814 at 2040.323 kb on - strand, within PP_1814 at 2040.409 kb on + strand, within PP_1814 at 2040.409 kb on + strand, within PP_1814 at 2040.431 kb on + strand at 2040.454 kb on + strand at 2040.477 kb on + strand at 2040.517 kb on + strand at 2040.517 kb on + strand at 2040.517 kb on + strand at 2040.518 kb on - strand at 2040.518 kb on - strand at 2040.518 kb on - strand at 2040.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 0.1 remove 2,039,097 + PP_1811 0.66 +0.3 2,039,097 + +0.8 2,039,098 - PP_1811 0.67 -0.7 2,039,098 - PP_1811 0.67 +1.6 2,039,098 - PP_1811 0.67 +0.8 2,039,126 + PP_1811 0.69 +0.6 2,039,126 + PP_1811 0.69 +0.4 2,039,127 - PP_1811 0.69 +0.1 2,039,151 + PP_1811 0.71 -2.1 2,039,151 + PP_1811 0.71 -0.9 2,039,154 + PP_1811 0.71 +0.0 2,039,154 + PP_1811 0.71 +0.7 2,039,155 - PP_1811 0.72 +0.3 2,039,155 - PP_1811 0.72 -0.6 2,039,155 - PP_1811 0.72 +1.0 2,039,155 - PP_1811 0.72 -0.3 2,039,307 + PP_1811 0.85 +0.2 2,039,336 + PP_1811 0.87 -0.3 2,039,581 - -1.5 2,039,632 + PP_1812 0.15 +0.4 2,039,688 + PP_1812 0.43 -0.5 2,039,689 - PP_1812 0.44 +0.3 2,039,811 + +0.6 2,039,820 - -0.4 2,039,917 - -1.0 2,040,072 + PP_1813 0.51 -1.4 2,040,120 + PP_1813 0.65 -0.5 2,040,121 - PP_1813 0.65 -0.9 2,040,189 - PP_1813 0.86 -1.6 2,040,235 + +0.7 2,040,235 + -0.3 2,040,236 - +0.4 2,040,313 - PP_1814 0.23 +1.4 2,040,313 - PP_1814 0.23 +1.0 2,040,322 + PP_1814 0.29 -0.8 2,040,322 + PP_1814 0.29 -0.0 2,040,322 + PP_1814 0.29 -0.2 2,040,323 - PP_1814 0.29 +1.0 2,040,409 + PP_1814 0.80 -1.4 2,040,409 + PP_1814 0.80 +1.7 2,040,431 + -0.1 2,040,454 + +0.3 2,040,477 + -0.2 2,040,517 + +0.9 2,040,517 + +0.1 2,040,517 + -1.1 2,040,518 - -0.3 2,040,518 - +0.4 2,040,518 - -1.6 2,040,520 - +0.4
Or see this region's nucleotide sequence