Strain Fitness in Pseudomonas putida KT2440 around PP_1431

Experiment: P. putida PUT10 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1430 and PP_1431 are separated by 180 nucleotidesPP_1431 and PP_1432 are separated by 5 nucleotides PP_1430: PP_1430 - putative periplasmic serine endoprotease DegP-like, at 1,629,588 to 1,631,066 _1430 PP_1431: PP_1431 - Elongation factor 4, at 1,631,247 to 1,633,046 _1431 PP_1432: PP_1432 - signal peptidase I, at 1,633,052 to 1,633,906 _1432 Position (kb) 1631 1632 1633 1634Strain fitness (log2 ratio) -2 -1 0 1 2at 1630.249 kb on + strand, within PP_1430at 1630.250 kb on - strand, within PP_1430at 1630.250 kb on - strand, within PP_1430at 1630.804 kb on + strandat 1630.804 kb on + strand, within PP_1430at 1630.805 kb on - strand, within PP_1430at 1631.064 kb on + strandat 1631.065 kb on - strandat 1631.144 kb on + strandat 1631.145 kb on - strandat 1631.182 kb on + strandat 1631.182 kb on + strandat 1631.442 kb on + strandat 1631.466 kb on + strand, within PP_1431at 1631.511 kb on + strand, within PP_1431at 1631.862 kb on + strandat 1632.231 kb on + strand, within PP_1431at 1632.773 kb on + strand, within PP_1431at 1633.040 kb on + strandat 1633.040 kb on + strandat 1633.040 kb on + strandat 1633.044 kb on + strandat 1633.954 kb on + strandat 1633.954 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 0.1
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1,630,249 + PP_1430 0.45 -0.1
1,630,250 - PP_1430 0.45 -0.5
1,630,250 - PP_1430 0.45 -0.1
1,630,804 + +2.7
1,630,804 + PP_1430 0.82 +0.4
1,630,805 - PP_1430 0.82 -2.3
1,631,064 + -1.4
1,631,065 - -1.3
1,631,144 + -0.8
1,631,145 - +0.7
1,631,182 + -1.0
1,631,182 + -2.0
1,631,442 + +1.7
1,631,466 + PP_1431 0.12 -0.6
1,631,511 + PP_1431 0.15 +1.7
1,631,862 + -0.6
1,632,231 + PP_1431 0.55 -0.6
1,632,773 + PP_1431 0.85 -0.9
1,633,040 + -1.9
1,633,040 + -2.1
1,633,040 + +0.1
1,633,044 + -2.1
1,633,954 + -0.5
1,633,954 + +0.7

Or see this region's nucleotide sequence