Strain Fitness in Pseudomonas putida KT2440 around PP_0518

Experiment: P. putida PUT10 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0516 and PP_0517 are separated by 91 nucleotidesPP_0517 and PP_0518 overlap by 4 nucleotidesPP_0518 and PP_0519 are separated by 18 nucleotidesPP_0519 and PP_0520 overlap by 4 nucleotides PP_0516: PP_0516 - 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II, at 602,495 to 603,604 _0516 PP_0517: PP_0517 - 6,7-dimethyl-8-ribityllumazine synthase, at 603,696 to 604,172 _0517 PP_0518: PP_0518 - transcription antitermination protein, at 604,169 to 604,669 _0518 PP_0519: PP_0519 - thiamin monophosphate kinase, at 604,688 to 605,656 _0519 PP_0520: PP_0520 - phosphatidylglycerophosphatase A, at 605,653 to 606,156 _0520 Position (kb) 604 605Strain fitness (log2 ratio) -1 0 1at 603.189 kb on - strand, within PP_0516at 603.189 kb on - strand, within PP_0516at 603.189 kb on - strand, within PP_0516at 603.467 kb on + strand, within PP_0516at 603.467 kb on + strand, within PP_0516at 603.467 kb on + strand, within PP_0516at 603.582 kb on + strandat 603.582 kb on + strandat 603.587 kb on + strandat 603.587 kb on + strandat 603.587 kb on + strandat 603.587 kb on + strandat 603.661 kb on + strandat 603.661 kb on + strandat 605.641 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 0.1
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603,189 - PP_0516 0.63 -0.0
603,189 - PP_0516 0.63 -0.1
603,189 - PP_0516 0.63 +0.2
603,467 + PP_0516 0.88 -0.2
603,467 + PP_0516 0.88 -0.1
603,467 + PP_0516 0.88 +0.5
603,582 + -1.1
603,582 + +0.4
603,587 + +0.2
603,587 + +0.6
603,587 + +0.1
603,587 + +0.1
603,661 + -1.1
603,661 + +0.7
605,641 + +0.8

Or see this region's nucleotide sequence