Experiment: P. putida PUT10 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0516 and PP_0517 are separated by 91 nucleotides PP_0517 and PP_0518 overlap by 4 nucleotides PP_0518 and PP_0519 are separated by 18 nucleotides PP_0519 and PP_0520 overlap by 4 nucleotides
PP_0516: PP_0516 - 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II, at 602,495 to 603,604
_0516
PP_0517: PP_0517 - 6,7-dimethyl-8-ribityllumazine synthase, at 603,696 to 604,172
_0517
PP_0518: PP_0518 - transcription antitermination protein, at 604,169 to 604,669
_0518
PP_0519: PP_0519 - thiamin monophosphate kinase, at 604,688 to 605,656
_0519
PP_0520: PP_0520 - phosphatidylglycerophosphatase A, at 605,653 to 606,156
_0520
Position (kb)
604
605 Strain fitness (log2 ratio)
-1
0
1 at 603.189 kb on - strand, within PP_0516 at 603.189 kb on - strand, within PP_0516 at 603.189 kb on - strand, within PP_0516 at 603.467 kb on + strand, within PP_0516 at 603.467 kb on + strand, within PP_0516 at 603.467 kb on + strand, within PP_0516 at 603.582 kb on + strand at 603.582 kb on + strand at 603.587 kb on + strand at 603.587 kb on + strand at 603.587 kb on + strand at 603.587 kb on + strand at 603.661 kb on + strand at 603.661 kb on + strand at 605.641 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 0.1 remove 603,189 - PP_0516 0.63 -0.0 603,189 - PP_0516 0.63 -0.1 603,189 - PP_0516 0.63 +0.2 603,467 + PP_0516 0.88 -0.2 603,467 + PP_0516 0.88 -0.1 603,467 + PP_0516 0.88 +0.5 603,582 + -1.1 603,582 + +0.4 603,587 + +0.2 603,587 + +0.6 603,587 + +0.1 603,587 + +0.1 603,661 + -1.1 603,661 + +0.7 605,641 + +0.8
Or see this region's nucleotide sequence