Strain Fitness in Pseudomonas putida KT2440 around PP_0442

Experiment: P. putida PUT10 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0440 and PP_t04 are separated by 54 nucleotidesPP_t04 and PP_0441 are separated by 44 nucleotidesPP_0441 and PP_0442 are separated by 9 nucleotidesPP_0442 and PP_0443 are separated by 119 nucleotidesPP_0443 and PP_0444 overlap by 1 nucleotides PP_0440: PP_0440 - Elongation factor Tu-A, at 532,736 to 533,929 _0440 PP_t04: PP_t04 - tRNA-Trp, at 533,984 to 534,059 _t04 PP_0441: PP_0441 - Sec translocation complex - SecE subunit, at 534,104 to 534,472 _0441 PP_0442: PP_0442 - transcription antipausing factor NusG, at 534,482 to 535,015 _0442 PP_0443: PP_0443 - 50S ribosomal protein L11, at 535,135 to 535,566 _0443 PP_0444: PP_0444 - 50S ribosomal protein L1, at 535,566 to 536,261 _0444 Position (kb) 534 535 536Strain fitness (log2 ratio) -1 0 1at 533.910 kb on + strandat 533.927 kb on + strandat 535.077 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 0.1
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533,910 + +0.2
533,927 + +0.1
535,077 + +1.7

Or see this region's nucleotide sequence