Experiment: P. putida PUT10 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5439 and PP_5440 are separated by 239 nucleotides PP_5440 and PP_t72 are separated by 188 nucleotides PP_t72 and PP_0285 are separated by 90 nucleotides PP_0285 and PP_0286 overlap by 4 nucleotides
PP_5439: PP_5439 - conserved protein of unknown function, at 343,001 to 343,444
_5439
PP_5440: PP_5440 - conserved protein of unknown function, at 343,684 to 343,911
_5440
PP_t72: PP_t72 - tRNA-Phe, at 344,100 to 344,175
_t72
PP_0285: PP_0285 - Fe(2+)-trafficking protein, at 344,266 to 344,538
_0285
PP_0286: PP_0286 - adenine glycosylase, at 344,535 to 345,602
_0286
Position (kb) 344
345 Strain fitness (log2 ratio) -2
-1
0
1
2 at 343.314 kb on + strand, within PP_5439 at 343.314 kb on + strand, within PP_5439 at 343.315 kb on - strand, within PP_5439 at 343.373 kb on + strand, within PP_5439 at 343.479 kb on - strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.549 kb on - strand at 343.549 kb on - strand at 343.549 kb on - strand at 343.553 kb on - strand at 343.553 kb on - strand at 343.561 kb on + strand at 343.583 kb on + strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.669 kb on + strand at 343.669 kb on + strand at 343.697 kb on - strand at 343.717 kb on + strand, within PP_5440 at 343.717 kb on + strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.741 kb on + strand, within PP_5440 at 343.742 kb on - strand, within PP_5440 at 343.779 kb on + strand, within PP_5440 at 343.779 kb on + strand, within PP_5440 at 343.780 kb on - strand, within PP_5440 at 343.852 kb on + strand, within PP_5440 at 343.852 kb on + strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.909 kb on + strand at 343.909 kb on + strand at 343.910 kb on - strand at 343.961 kb on + strand at 343.962 kb on - strand at 344.286 kb on - strand at 344.511 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.861 kb on - strand, within PP_0286 at 345.026 kb on + strand, within PP_0286 at 345.064 kb on + strand, within PP_0286 at 345.065 kb on - strand, within PP_0286 at 345.089 kb on + strand, within PP_0286 at 345.111 kb on + strand, within PP_0286 at 345.111 kb on + strand, within PP_0286 at 345.112 kb on - strand, within PP_0286 at 345.112 kb on - strand, within PP_0286 at 345.112 kb on - strand at 345.160 kb on + strand, within PP_0286 at 345.160 kb on + strand, within PP_0286 at 345.161 kb on - strand, within PP_0286 at 345.270 kb on - strand, within PP_0286 at 345.355 kb on + strand, within PP_0286 at 345.355 kb on + strand, within PP_0286 at 345.356 kb on - strand, within PP_0286 at 345.356 kb on - strand, within PP_0286 at 345.356 kb on - strand, within PP_0286 at 345.358 kb on + strand, within PP_0286 at 345.451 kb on + strand, within PP_0286 at 345.452 kb on - strand, within PP_0286 at 345.452 kb on - strand, within PP_0286 at 345.452 kb on - strand, within PP_0286
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 0.1 remove 343,314 + PP_5439 0.70 -1.5 343,314 + PP_5439 0.70 -2.6 343,315 - PP_5439 0.71 -1.9 343,373 + PP_5439 0.84 +0.5 343,479 - -0.2 343,548 + -0.3 343,548 + +1.2 343,548 + +1.5 343,548 + +0.0 343,548 + -1.0 343,548 + -0.7 343,549 - -0.2 343,549 - -1.4 343,549 - -2.4 343,553 - -0.1 343,553 - -0.6 343,561 + +0.3 343,583 + -0.3 343,584 - -0.7 343,584 - +0.8 343,584 - -0.0 343,584 - +2.0 343,669 + -0.0 343,669 + +0.7 343,697 - +0.2 343,717 + PP_5440 0.14 +0.7 343,717 + PP_5440 0.14 -0.4 343,739 - PP_5440 0.24 -1.8 343,739 - PP_5440 0.24 -1.2 343,739 - PP_5440 0.24 -0.3 343,739 - PP_5440 0.24 -1.2 343,741 + PP_5440 0.25 -0.2 343,742 - PP_5440 0.25 +0.7 343,779 + PP_5440 0.42 +0.4 343,779 + PP_5440 0.42 +0.1 343,780 - PP_5440 0.42 -1.0 343,852 + PP_5440 0.74 +0.5 343,852 + PP_5440 0.74 +0.1 343,853 - PP_5440 0.74 -1.1 343,853 - PP_5440 0.74 -0.9 343,853 - PP_5440 0.74 -1.6 343,909 + +0.2 343,909 + +0.6 343,910 - -0.7 343,961 + -1.0 343,962 - -0.8 344,286 - +0.1 344,511 - +0.9 344,633 - -0.0 344,633 - +0.7 344,633 - +0.4 344,633 - -1.0 344,861 - PP_0286 0.31 +0.3 345,026 + PP_0286 0.46 -1.2 345,064 + PP_0286 0.50 -0.1 345,065 - PP_0286 0.50 +0.3 345,089 + PP_0286 0.52 +1.8 345,111 + PP_0286 0.54 -0.1 345,111 + PP_0286 0.54 -1.3 345,112 - PP_0286 0.54 -0.3 345,112 - PP_0286 0.54 -1.3 345,112 - +1.8 345,160 + PP_0286 0.59 -0.6 345,160 + PP_0286 0.59 -0.2 345,161 - PP_0286 0.59 +0.5 345,270 - PP_0286 0.69 +0.3 345,355 + PP_0286 0.77 -0.2 345,355 + PP_0286 0.77 -0.4 345,356 - PP_0286 0.77 +1.1 345,356 - PP_0286 0.77 -1.0 345,356 - PP_0286 0.77 +0.6 345,358 + PP_0286 0.77 +0.7 345,451 + PP_0286 0.86 -1.7 345,452 - PP_0286 0.86 -0.4 345,452 - PP_0286 0.86 +0.4 345,452 - PP_0286 0.86 +0.4
Or see this region's nucleotide sequence