Strain Fitness in Pseudomonas putida KT2440 around PP_t69

Experiment: P. putida PUT10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0722 and PP_t69 are separated by 48 nucleotidesPP_t69 and PP_0723 are separated by 20 nucleotidesPP_0723 and PP_0724 are separated by 4 nucleotides PP_0722: PP_0722 - Ribose-phosphate pyrophosphokinase, at 838,756 to 839,697 _0722 PP_t69: PP_t69 - tRNA-Gln, at 839,746 to 839,820 _t69 PP_0723: PP_0723 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 839,841 to 840,701 _0723 PP_0724: PP_0724 - Outer-membrane lipoprotein LolB, at 840,706 to 841,323 _0724 Position (kb) 839 840Strain fitness (log2 ratio) -1 0 1 2at 839.722 kb on - strandat 839.722 kb on - strandat 839.724 kb on - strandat 839.724 kb on - strandat 839.736 kb on - strandat 839.738 kb on - strandat 839.877 kb on - strandat 839.877 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1
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839,722 - +0.9
839,722 - +0.1
839,724 - +0.8
839,724 - +0.9
839,736 - +2.4
839,738 - +0.9
839,877 - +2.1
839,877 - -0.5

Or see this region's nucleotide sequence