Experiment: P. putida PUT10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5440 and PP_t72 are separated by 188 nucleotides PP_t72 and PP_0285 are separated by 90 nucleotides PP_0285 and PP_0286 overlap by 4 nucleotides PP_0286 and PP_0287 overlap by 4 nucleotides
PP_5440: PP_5440 - conserved protein of unknown function, at 343,684 to 343,911
_5440
PP_t72: PP_t72 - tRNA-Phe, at 344,100 to 344,175
_t72
PP_0285: PP_0285 - Fe(2+)-trafficking protein, at 344,266 to 344,538
_0285
PP_0286: PP_0286 - adenine glycosylase, at 344,535 to 345,602
_0286
PP_0287: PP_0287 - conserved exported protein of unknown function, at 345,599 to 347,845
_0287
Position (kb) 344
345
346 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.549 kb on - strand at 343.549 kb on - strand at 343.549 kb on - strand at 343.553 kb on - strand at 343.553 kb on - strand at 343.561 kb on + strand at 343.583 kb on + strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.669 kb on + strand at 343.669 kb on + strand at 343.697 kb on - strand at 343.717 kb on + strand, within PP_5440 at 343.717 kb on + strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.741 kb on + strand, within PP_5440 at 343.742 kb on - strand, within PP_5440 at 343.779 kb on + strand, within PP_5440 at 343.779 kb on + strand, within PP_5440 at 343.780 kb on - strand, within PP_5440 at 343.852 kb on + strand, within PP_5440 at 343.852 kb on + strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.909 kb on + strand at 343.909 kb on + strand at 343.910 kb on - strand at 343.961 kb on + strand at 343.962 kb on - strand at 344.286 kb on - strand at 344.511 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.861 kb on - strand, within PP_0286 at 345.026 kb on + strand, within PP_0286 at 345.064 kb on + strand, within PP_0286 at 345.065 kb on - strand, within PP_0286 at 345.089 kb on + strand, within PP_0286 at 345.111 kb on + strand, within PP_0286 at 345.111 kb on + strand, within PP_0286 at 345.112 kb on - strand, within PP_0286 at 345.112 kb on - strand, within PP_0286 at 345.112 kb on - strand at 345.160 kb on + strand, within PP_0286 at 345.160 kb on + strand, within PP_0286 at 345.161 kb on - strand, within PP_0286 at 345.270 kb on - strand, within PP_0286 at 345.355 kb on + strand, within PP_0286 at 345.355 kb on + strand, within PP_0286 at 345.356 kb on - strand, within PP_0286 at 345.356 kb on - strand, within PP_0286 at 345.356 kb on - strand, within PP_0286 at 345.358 kb on + strand, within PP_0286 at 345.451 kb on + strand, within PP_0286 at 345.452 kb on - strand, within PP_0286 at 345.452 kb on - strand, within PP_0286 at 345.452 kb on - strand, within PP_0286 at 345.562 kb on + strand at 345.810 kb on + strand at 345.810 kb on + strand at 345.811 kb on - strand at 345.811 kb on - strand at 345.882 kb on + strand, within PP_0287 at 345.882 kb on + strand, within PP_0287 at 345.882 kb on + strand, within PP_0287 at 345.882 kb on - strand, within PP_0287 at 345.883 kb on - strand, within PP_0287 at 345.883 kb on - strand, within PP_0287 at 345.883 kb on - strand, within PP_0287 at 346.353 kb on + strand, within PP_0287 at 346.353 kb on + strand, within PP_0287 at 346.353 kb on + strand, within PP_0287 at 346.353 kb on + strand at 346.354 kb on - strand, within PP_0287 at 346.354 kb on - strand, within PP_0287 at 346.436 kb on - strand, within PP_0287
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1 remove 343,548 + +0.3 343,548 + +1.1 343,548 + +1.7 343,548 + +0.2 343,548 + -0.0 343,548 + -0.9 343,549 - +0.3 343,549 - -0.5 343,549 - -0.4 343,553 - -0.2 343,553 - -0.8 343,561 + -0.2 343,583 + +0.5 343,584 - -1.2 343,584 - -0.2 343,584 - -0.2 343,584 - -1.2 343,669 + +1.2 343,669 + -0.5 343,697 - -1.2 343,717 + PP_5440 0.14 -0.5 343,717 + PP_5440 0.14 +0.2 343,739 - PP_5440 0.24 -0.0 343,739 - PP_5440 0.24 -1.8 343,739 - PP_5440 0.24 -0.7 343,739 - PP_5440 0.24 -0.2 343,741 + PP_5440 0.25 +0.5 343,742 - PP_5440 0.25 +0.1 343,779 + PP_5440 0.42 -0.2 343,779 + PP_5440 0.42 -1.3 343,780 - PP_5440 0.42 +0.0 343,852 + PP_5440 0.74 +0.1 343,852 + PP_5440 0.74 -0.4 343,853 - PP_5440 0.74 -1.8 343,853 - PP_5440 0.74 +0.1 343,853 - PP_5440 0.74 -0.8 343,909 + +0.6 343,909 + -0.2 343,910 - -1.5 343,961 + -0.6 343,962 - -0.4 344,286 - +0.1 344,511 - +0.3 344,633 - -0.3 344,633 - -0.5 344,633 - -1.2 344,633 - -0.0 344,861 - PP_0286 0.31 -0.5 345,026 + PP_0286 0.46 -1.6 345,064 + PP_0286 0.50 -1.5 345,065 - PP_0286 0.50 -1.0 345,089 + PP_0286 0.52 -0.9 345,111 + PP_0286 0.54 -1.3 345,111 + PP_0286 0.54 -0.7 345,112 - PP_0286 0.54 -0.9 345,112 - PP_0286 0.54 +0.5 345,112 - +0.4 345,160 + PP_0286 0.59 -1.0 345,160 + PP_0286 0.59 -0.2 345,161 - PP_0286 0.59 -0.3 345,270 - PP_0286 0.69 -2.0 345,355 + PP_0286 0.77 -0.4 345,355 + PP_0286 0.77 -0.3 345,356 - PP_0286 0.77 +0.7 345,356 - PP_0286 0.77 -0.4 345,356 - PP_0286 0.77 +0.4 345,358 + PP_0286 0.77 -1.8 345,451 + PP_0286 0.86 +0.3 345,452 - PP_0286 0.86 -0.4 345,452 - PP_0286 0.86 -0.3 345,452 - PP_0286 0.86 -0.5 345,562 + +0.9 345,810 + +2.4 345,810 + -0.1 345,811 - +0.6 345,811 - -0.8 345,882 + PP_0287 0.13 +1.4 345,882 + PP_0287 0.13 -0.3 345,882 + PP_0287 0.13 -3.3 345,882 - PP_0287 0.13 -2.0 345,883 - PP_0287 0.13 -0.4 345,883 - PP_0287 0.13 -1.2 345,883 - PP_0287 0.13 +1.3 346,353 + PP_0287 0.34 -2.6 346,353 + PP_0287 0.34 +0.4 346,353 + PP_0287 0.34 -1.9 346,353 + +1.4 346,354 - PP_0287 0.34 +1.4 346,354 - PP_0287 0.34 -0.2 346,436 - PP_0287 0.37 -1.9
Or see this region's nucleotide sequence