Experiment: P. putida PUT10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0041 and PP_0042 are separated by 202 nucleotides PP_0042 and PP_0043 are separated by 162 nucleotides PP_0043 and PP_0044 are separated by 24 nucleotides
PP_0041: PP_0041 - cadmium translocating P-type ATPase, at 43,527 to 45,524
_0041
PP_0042: PP_0042 - conserved exported protein of unknown function, at 45,727 to 46,050
_0042
PP_0043: PP_0043 - cation efflux system protein, at 46,213 to 49,374
_0043
PP_0044: PP_0044 - cobalt-zinc-cadmium resistance protein, at 49,399 to 50,649
_0044
Position (kb) 46
47
48
49
50 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 45.293 kb on + strand, within PP_0041 at 45.305 kb on + strand, within PP_0041 at 45.332 kb on - strand at 45.332 kb on - strand at 45.332 kb on - strand at 45.343 kb on + strand at 45.344 kb on - strand at 45.344 kb on - strand at 45.344 kb on - strand at 45.344 kb on - strand at 45.473 kb on + strand at 45.474 kb on - strand at 45.525 kb on - strand at 45.525 kb on - strand at 45.595 kb on + strand at 45.602 kb on + strand at 45.602 kb on + strand at 45.646 kb on - strand at 45.815 kb on + strand, within PP_0042 at 45.815 kb on + strand, within PP_0042 at 45.815 kb on + strand, within PP_0042 at 45.816 kb on - strand, within PP_0042 at 45.828 kb on + strand, within PP_0042 at 45.829 kb on - strand, within PP_0042 at 45.868 kb on + strand, within PP_0042 at 45.868 kb on + strand, within PP_0042 at 45.869 kb on - strand, within PP_0042 at 45.881 kb on + strand, within PP_0042 at 45.882 kb on - strand, within PP_0042 at 45.882 kb on - strand, within PP_0042 at 45.962 kb on + strand, within PP_0042 at 45.963 kb on - strand, within PP_0042 at 46.089 kb on + strand at 46.090 kb on - strand at 46.214 kb on + strand at 46.215 kb on - strand at 46.219 kb on - strand at 46.268 kb on + strand at 46.268 kb on + strand at 46.268 kb on + strand at 46.283 kb on - strand at 46.525 kb on + strand at 46.526 kb on - strand at 46.526 kb on - strand at 46.779 kb on - strand, within PP_0043 at 47.023 kb on + strand, within PP_0043 at 47.024 kb on - strand, within PP_0043 at 47.024 kb on - strand, within PP_0043 at 47.053 kb on + strand, within PP_0043 at 47.053 kb on + strand, within PP_0043 at 47.053 kb on + strand, within PP_0043 at 47.053 kb on + strand, within PP_0043 at 47.053 kb on + strand, within PP_0043 at 47.053 kb on + strand, within PP_0043 at 47.054 kb on - strand, within PP_0043 at 47.054 kb on - strand, within PP_0043 at 47.054 kb on - strand, within PP_0043 at 47.056 kb on + strand, within PP_0043 at 47.056 kb on + strand, within PP_0043 at 47.056 kb on + strand, within PP_0043 at 47.056 kb on + strand, within PP_0043 at 47.056 kb on + strand, within PP_0043 at 47.056 kb on + strand, within PP_0043 at 47.057 kb on - strand, within PP_0043 at 47.057 kb on - strand, within PP_0043 at 47.057 kb on - strand, within PP_0043 at 47.057 kb on - strand, within PP_0043 at 47.057 kb on - strand, within PP_0043 at 47.057 kb on - strand, within PP_0043 at 47.057 kb on - strand, within PP_0043 at 47.057 kb on - strand, within PP_0043 at 47.084 kb on + strand, within PP_0043 at 47.084 kb on + strand, within PP_0043 at 47.085 kb on - strand, within PP_0043 at 47.085 kb on - strand, within PP_0043 at 47.328 kb on + strand, within PP_0043 at 47.328 kb on + strand, within PP_0043 at 47.329 kb on - strand, within PP_0043 at 47.384 kb on + strand, within PP_0043 at 47.384 kb on + strand, within PP_0043 at 47.385 kb on - strand, within PP_0043 at 47.385 kb on - strand, within PP_0043 at 47.457 kb on + strand, within PP_0043 at 47.490 kb on - strand, within PP_0043 at 47.490 kb on - strand, within PP_0043 at 47.490 kb on - strand, within PP_0043 at 47.703 kb on + strand, within PP_0043 at 47.704 kb on - strand, within PP_0043 at 47.799 kb on - strand, within PP_0043 at 47.868 kb on + strand, within PP_0043 at 47.868 kb on + strand, within PP_0043 at 47.868 kb on + strand, within PP_0043 at 47.868 kb on + strand, within PP_0043 at 47.868 kb on + strand, within PP_0043 at 47.869 kb on - strand, within PP_0043 at 47.869 kb on - strand, within PP_0043 at 47.988 kb on - strand, within PP_0043 at 47.988 kb on - strand, within PP_0043 at 48.096 kb on + strand, within PP_0043 at 48.096 kb on + strand, within PP_0043 at 48.096 kb on + strand, within PP_0043 at 48.096 kb on + strand, within PP_0043 at 48.096 kb on + strand, within PP_0043 at 48.096 kb on + strand, within PP_0043 at 48.096 kb on + strand, within PP_0043 at 48.096 kb on + strand, within PP_0043 at 48.096 kb on + strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.097 kb on - strand, within PP_0043 at 48.138 kb on + strand, within PP_0043 at 48.138 kb on + strand, within PP_0043 at 48.190 kb on + strand, within PP_0043 at 48.191 kb on - strand, within PP_0043 at 48.191 kb on - strand, within PP_0043 at 48.378 kb on + strand, within PP_0043 at 48.386 kb on + strand, within PP_0043 at 48.387 kb on - strand, within PP_0043 at 48.460 kb on + strand, within PP_0043 at 48.460 kb on + strand, within PP_0043 at 48.460 kb on + strand, within PP_0043 at 48.461 kb on - strand, within PP_0043 at 48.528 kb on + strand, within PP_0043 at 48.528 kb on + strand, within PP_0043 at 48.528 kb on + strand, within PP_0043 at 48.540 kb on + strand, within PP_0043 at 48.540 kb on + strand, within PP_0043 at 48.540 kb on + strand, within PP_0043 at 48.552 kb on + strand, within PP_0043 at 48.553 kb on - strand, within PP_0043 at 48.637 kb on - strand, within PP_0043 at 48.643 kb on + strand, within PP_0043 at 48.644 kb on - strand, within PP_0043 at 48.644 kb on - strand, within PP_0043 at 48.681 kb on + strand, within PP_0043 at 48.682 kb on - strand, within PP_0043 at 48.758 kb on + strand, within PP_0043 at 48.758 kb on + strand, within PP_0043 at 48.758 kb on + strand, within PP_0043 at 48.759 kb on - strand, within PP_0043 at 48.819 kb on + strand, within PP_0043 at 48.820 kb on - strand, within PP_0043 at 48.902 kb on + strand, within PP_0043 at 48.910 kb on - strand, within PP_0043 at 48.910 kb on - strand, within PP_0043 at 48.910 kb on - strand, within PP_0043 at 48.975 kb on + strand, within PP_0043 at 48.975 kb on + strand, within PP_0043 at 48.976 kb on - strand, within PP_0043 at 48.976 kb on - strand, within PP_0043 at 48.976 kb on - strand, within PP_0043 at 48.980 kb on - strand, within PP_0043 at 49.000 kb on + strand, within PP_0043 at 49.251 kb on + strand at 49.283 kb on + strand at 49.284 kb on - strand at 49.284 kb on - strand at 49.289 kb on + strand at 49.289 kb on + strand at 49.289 kb on + strand at 49.289 kb on + strand at 49.289 kb on + strand at 49.289 kb on + strand at 49.289 kb on + strand at 49.289 kb on + strand at 49.289 kb on + strand at 49.289 kb on + strand at 49.290 kb on - strand at 49.290 kb on - strand at 49.290 kb on - strand at 49.290 kb on - strand at 49.290 kb on - strand at 49.290 kb on - strand at 49.290 kb on - strand at 49.290 kb on - strand at 49.294 kb on - strand at 49.327 kb on + strand at 49.327 kb on + strand at 49.327 kb on + strand at 49.327 kb on + strand at 49.327 kb on + strand at 49.328 kb on - strand at 49.328 kb on - strand at 49.330 kb on + strand at 49.362 kb on + strand at 49.362 kb on + strand at 49.362 kb on + strand at 49.362 kb on + strand at 49.363 kb on - strand at 49.363 kb on - strand at 49.363 kb on - strand at 49.363 kb on - strand at 49.363 kb on - strand at 49.465 kb on + strand at 49.466 kb on - strand at 49.466 kb on - strand at 49.485 kb on + strand at 49.485 kb on + strand at 49.486 kb on - strand at 49.506 kb on - strand at 49.506 kb on - strand at 49.506 kb on - strand at 49.564 kb on + strand, within PP_0044 at 49.564 kb on + strand, within PP_0044 at 49.564 kb on + strand, within PP_0044 at 49.565 kb on - strand, within PP_0044 at 49.658 kb on - strand, within PP_0044 at 49.713 kb on + strand, within PP_0044 at 49.713 kb on + strand, within PP_0044 at 49.713 kb on + strand, within PP_0044 at 49.713 kb on + strand, within PP_0044 at 49.713 kb on + strand, within PP_0044 at 49.714 kb on - strand, within PP_0044 at 49.714 kb on - strand, within PP_0044 at 49.751 kb on + strand, within PP_0044 at 49.751 kb on + strand, within PP_0044 at 49.752 kb on - strand, within PP_0044 at 49.752 kb on - strand, within PP_0044 at 49.940 kb on - strand, within PP_0044 at 49.940 kb on - strand, within PP_0044 at 49.970 kb on + strand, within PP_0044 at 49.992 kb on + strand, within PP_0044 at 50.302 kb on - strand, within PP_0044
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1 remove 45,293 + PP_0041 0.88 +0.4 45,305 + PP_0041 0.89 +0.5 45,332 - -0.2 45,332 - -1.1 45,332 - -0.5 45,343 + -0.5 45,344 - -0.5 45,344 - -0.9 45,344 - +0.2 45,344 - -1.3 45,473 + +0.1 45,474 - -0.9 45,525 - -0.6 45,525 - -0.8 45,595 + +0.0 45,602 + +0.4 45,602 + +0.6 45,646 - +0.6 45,815 + PP_0042 0.27 -0.2 45,815 + PP_0042 0.27 -1.2 45,815 + PP_0042 0.27 -0.8 45,816 - PP_0042 0.27 -5.2 45,828 + PP_0042 0.31 -0.5 45,829 - PP_0042 0.31 -3.4 45,868 + PP_0042 0.44 +0.1 45,868 + PP_0042 0.44 +0.6 45,869 - PP_0042 0.44 -4.1 45,881 + PP_0042 0.48 -1.4 45,882 - PP_0042 0.48 -3.6 45,882 - PP_0042 0.48 -0.0 45,962 + PP_0042 0.73 -0.1 45,963 - PP_0042 0.73 -2.6 46,089 + -0.2 46,090 - -4.1 46,214 + +0.1 46,215 - +0.2 46,219 - -1.3 46,268 + -0.0 46,268 + -0.0 46,268 + -1.1 46,283 - +0.1 46,525 + +0.3 46,526 - +1.4 46,526 - +0.1 46,779 - PP_0043 0.18 -0.5 47,023 + PP_0043 0.26 -0.2 47,024 - PP_0043 0.26 +0.6 47,024 - PP_0043 0.26 -1.1 47,053 + PP_0043 0.27 -1.0 47,053 + PP_0043 0.27 -1.5 47,053 + PP_0043 0.27 -0.1 47,053 + PP_0043 0.27 -0.0 47,053 + PP_0043 0.27 -0.4 47,053 + PP_0043 0.27 -0.3 47,054 - PP_0043 0.27 -0.6 47,054 - PP_0043 0.27 +1.2 47,054 - PP_0043 0.27 +0.4 47,056 + PP_0043 0.27 +0.7 47,056 + PP_0043 0.27 -0.4 47,056 + PP_0043 0.27 +0.5 47,056 + PP_0043 0.27 +0.3 47,056 + PP_0043 0.27 -0.3 47,056 + PP_0043 0.27 +0.4 47,057 - PP_0043 0.27 -0.2 47,057 - PP_0043 0.27 +0.2 47,057 - PP_0043 0.27 -0.2 47,057 - PP_0043 0.27 +0.5 47,057 - PP_0043 0.27 -1.0 47,057 - PP_0043 0.27 -0.5 47,057 - PP_0043 0.27 -0.1 47,057 - PP_0043 0.27 -0.0 47,084 + PP_0043 0.28 -0.6 47,084 + PP_0043 0.28 -0.5 47,085 - PP_0043 0.28 +0.4 47,085 - PP_0043 0.28 -0.3 47,328 + PP_0043 0.35 -0.1 47,328 + PP_0043 0.35 +1.4 47,329 - PP_0043 0.35 -0.4 47,384 + PP_0043 0.37 -0.8 47,384 + PP_0043 0.37 -0.5 47,385 - PP_0043 0.37 +0.8 47,385 - PP_0043 0.37 -0.1 47,457 + PP_0043 0.39 -0.3 47,490 - PP_0043 0.40 +0.2 47,490 - PP_0043 0.40 -0.3 47,490 - PP_0043 0.40 -0.7 47,703 + PP_0043 0.47 -0.9 47,704 - PP_0043 0.47 -0.6 47,799 - PP_0043 0.50 +1.7 47,868 + PP_0043 0.52 -0.9 47,868 + PP_0043 0.52 -0.4 47,868 + PP_0043 0.52 +0.2 47,868 + PP_0043 0.52 -0.5 47,868 + PP_0043 0.52 +1.2 47,869 - PP_0043 0.52 -1.4 47,869 - PP_0043 0.52 -1.0 47,988 - PP_0043 0.56 -0.8 47,988 - PP_0043 0.56 -0.4 48,096 + PP_0043 0.60 -1.4 48,096 + PP_0043 0.60 -0.7 48,096 + PP_0043 0.60 +0.1 48,096 + PP_0043 0.60 -0.6 48,096 + PP_0043 0.60 -2.4 48,096 + PP_0043 0.60 -0.9 48,096 + PP_0043 0.60 -2.4 48,096 + PP_0043 0.60 +0.4 48,096 + PP_0043 0.60 +0.5 48,097 - PP_0043 0.60 -0.4 48,097 - PP_0043 0.60 +0.0 48,097 - PP_0043 0.60 +0.1 48,097 - PP_0043 0.60 -0.6 48,097 - PP_0043 0.60 -1.2 48,097 - PP_0043 0.60 +0.4 48,097 - PP_0043 0.60 -1.3 48,097 - PP_0043 0.60 +0.4 48,097 - PP_0043 0.60 -0.5 48,097 - PP_0043 0.60 -0.8 48,097 - PP_0043 0.60 -0.7 48,097 - PP_0043 0.60 -0.9 48,097 - PP_0043 0.60 -0.6 48,097 - PP_0043 0.60 -0.1 48,138 + PP_0043 0.61 -0.3 48,138 + PP_0043 0.61 +0.4 48,190 + PP_0043 0.63 +0.4 48,191 - PP_0043 0.63 -1.8 48,191 - PP_0043 0.63 -0.3 48,378 + PP_0043 0.68 -1.4 48,386 + PP_0043 0.69 -0.4 48,387 - PP_0043 0.69 +1.1 48,460 + PP_0043 0.71 +1.2 48,460 + PP_0043 0.71 -0.5 48,460 + PP_0043 0.71 -0.8 48,461 - PP_0043 0.71 -1.2 48,528 + PP_0043 0.73 -0.0 48,528 + PP_0043 0.73 -1.6 48,528 + PP_0043 0.73 +0.2 48,540 + PP_0043 0.74 -1.0 48,540 + PP_0043 0.74 -0.6 48,540 + PP_0043 0.74 -0.7 48,552 + PP_0043 0.74 -1.2 48,553 - PP_0043 0.74 -1.2 48,637 - PP_0043 0.77 -2.1 48,643 + PP_0043 0.77 +0.4 48,644 - PP_0043 0.77 +0.1 48,644 - PP_0043 0.77 -0.2 48,681 + PP_0043 0.78 -0.3 48,682 - PP_0043 0.78 -0.1 48,758 + PP_0043 0.80 -0.8 48,758 + PP_0043 0.80 -0.7 48,758 + PP_0043 0.80 -1.2 48,759 - PP_0043 0.81 -2.5 48,819 + PP_0043 0.82 +0.6 48,820 - PP_0043 0.82 -0.9 48,902 + PP_0043 0.85 +0.1 48,910 - PP_0043 0.85 -0.1 48,910 - PP_0043 0.85 +0.6 48,910 - PP_0043 0.85 -1.8 48,975 + PP_0043 0.87 +0.1 48,975 + PP_0043 0.87 +0.2 48,976 - PP_0043 0.87 -0.2 48,976 - PP_0043 0.87 +0.2 48,976 - PP_0043 0.87 +0.8 48,980 - PP_0043 0.88 +0.1 49,000 + PP_0043 0.88 -0.1 49,251 + +0.8 49,283 + -0.7 49,284 - +0.2 49,284 - +0.4 49,289 + -0.1 49,289 + +0.1 49,289 + -0.6 49,289 + -2.0 49,289 + -0.2 49,289 + -0.1 49,289 + -0.8 49,289 + -1.2 49,289 + -0.5 49,289 + -0.6 49,290 - -0.7 49,290 - -0.6 49,290 - -0.6 49,290 - -0.1 49,290 - +0.1 49,290 - +0.1 49,290 - +0.6 49,290 - -1.7 49,294 - -0.0 49,327 + -0.1 49,327 + -0.2 49,327 + +0.1 49,327 + +0.3 49,327 + -0.3 49,328 - +0.2 49,328 - -0.4 49,330 + +0.7 49,362 + +0.4 49,362 + +0.6 49,362 + +0.3 49,362 + -0.6 49,363 - -1.2 49,363 - -0.0 49,363 - -0.4 49,363 - -0.5 49,363 - -0.9 49,465 + -0.6 49,466 - -0.7 49,466 - -1.8 49,485 + -0.2 49,485 + +0.0 49,486 - -1.2 49,506 - +0.1 49,506 - -0.7 49,506 - +0.3 49,564 + PP_0044 0.13 +1.3 49,564 + PP_0044 0.13 +0.8 49,564 + PP_0044 0.13 +0.7 49,565 - PP_0044 0.13 -0.7 49,658 - PP_0044 0.21 +0.7 49,713 + PP_0044 0.25 -0.2 49,713 + PP_0044 0.25 +0.7 49,713 + PP_0044 0.25 -0.6 49,713 + PP_0044 0.25 -0.5 49,713 + PP_0044 0.25 +0.1 49,714 - PP_0044 0.25 -1.4 49,714 - PP_0044 0.25 -0.2 49,751 + PP_0044 0.28 +0.6 49,751 + PP_0044 0.28 +0.6 49,752 - PP_0044 0.28 +0.6 49,752 - PP_0044 0.28 -0.0 49,940 - PP_0044 0.43 -0.1 49,940 - PP_0044 0.43 +0.0 49,970 + PP_0044 0.46 +0.9 49,992 + PP_0044 0.47 -0.5 50,302 - PP_0044 0.72 -0.2
Or see this region's nucleotide sequence