Experiment: carbon source D-Mannose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfL and msrA are separated by 322 nucleotides msrA and ytfM are separated by 205 nucleotides
b4218: ytfL - predicted inner membrane protein (NCBI), at 4,437,895 to 4,439,238
ytfL
b4219: msrA - methionine sulfoxide reductase A (NCBI), at 4,439,561 to 4,440,199
msrA
b4220: ytfM - predicted outer membrane protein and surface antigen (NCBI), at 4,440,405 to 4,442,138
ytfM
Position (kb)
4439
4440
4441 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4438.568 kb on - strand, within ytfL at 4438.595 kb on - strand, within ytfL at 4438.595 kb on - strand, within ytfL at 4438.694 kb on - strand, within ytfL at 4438.702 kb on - strand, within ytfL at 4438.731 kb on + strand, within ytfL at 4438.739 kb on - strand, within ytfL at 4438.739 kb on - strand, within ytfL at 4438.755 kb on + strand, within ytfL at 4438.755 kb on + strand, within ytfL at 4438.762 kb on - strand, within ytfL at 4438.762 kb on - strand, within ytfL at 4438.775 kb on + strand, within ytfL at 4438.775 kb on + strand, within ytfL at 4438.826 kb on - strand, within ytfL at 4438.907 kb on + strand, within ytfL at 4438.907 kb on + strand, within ytfL at 4439.009 kb on + strand, within ytfL at 4439.049 kb on - strand, within ytfL at 4439.071 kb on - strand, within ytfL at 4439.201 kb on - strand at 4439.208 kb on + strand at 4439.212 kb on + strand at 4439.212 kb on + strand at 4439.226 kb on - strand at 4439.345 kb on + strand at 4439.345 kb on + strand at 4439.355 kb on - strand at 4439.370 kb on - strand at 4439.431 kb on - strand at 4439.431 kb on - strand at 4439.466 kb on - strand at 4439.688 kb on + strand, within msrA at 4439.739 kb on + strand, within msrA at 4439.759 kb on + strand, within msrA at 4439.759 kb on + strand, within msrA at 4439.764 kb on + strand, within msrA at 4439.872 kb on + strand, within msrA at 4439.937 kb on - strand, within msrA at 4439.937 kb on - strand, within msrA at 4440.009 kb on + strand, within msrA at 4440.090 kb on + strand, within msrA at 4440.120 kb on - strand, within msrA at 4440.131 kb on - strand, within msrA at 4440.143 kb on - strand at 4440.143 kb on - strand at 4440.279 kb on - strand at 4440.331 kb on + strand at 4440.331 kb on + strand at 4440.430 kb on + strand at 4440.435 kb on + strand at 4440.435 kb on + strand at 4440.435 kb on + strand at 4440.483 kb on - strand at 4440.483 kb on - strand at 4440.485 kb on - strand at 4440.582 kb on + strand, within ytfM at 4440.582 kb on - strand, within ytfM at 4440.675 kb on - strand, within ytfM at 4440.681 kb on + strand, within ytfM at 4440.698 kb on + strand, within ytfM at 4440.740 kb on + strand, within ytfM at 4440.741 kb on - strand, within ytfM at 4440.741 kb on - strand, within ytfM at 4440.770 kb on - strand, within ytfM at 4440.770 kb on - strand, within ytfM at 4440.798 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.818 kb on - strand, within ytfM at 4440.861 kb on + strand, within ytfM at 4440.864 kb on - strand, within ytfM at 4440.864 kb on - strand, within ytfM at 4440.935 kb on + strand, within ytfM at 4440.935 kb on + strand, within ytfM at 4440.948 kb on + strand, within ytfM at 4440.973 kb on + strand, within ytfM at 4440.976 kb on - strand, within ytfM at 4440.989 kb on + strand, within ytfM at 4440.996 kb on - strand, within ytfM at 4441.096 kb on + strand, within ytfM at 4441.144 kb on - strand, within ytfM
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM remove 4,438,568 - ytfL b4218 0.50 -0.2 4,438,595 - ytfL b4218 0.52 -0.3 4,438,595 - ytfL b4218 0.52 -0.5 4,438,694 - ytfL b4218 0.59 +0.4 4,438,702 - ytfL b4218 0.60 +1.0 4,438,731 + ytfL b4218 0.62 -1.3 4,438,739 - ytfL b4218 0.63 +2.2 4,438,739 - ytfL b4218 0.63 -1.9 4,438,755 + ytfL b4218 0.64 -0.0 4,438,755 + ytfL b4218 0.64 -0.6 4,438,762 - ytfL b4218 0.65 +0.3 4,438,762 - ytfL b4218 0.65 -1.3 4,438,775 + ytfL b4218 0.65 -3.4 4,438,775 + ytfL b4218 0.65 -0.8 4,438,826 - ytfL b4218 0.69 -4.1 4,438,907 + ytfL b4218 0.75 +2.0 4,438,907 + ytfL b4218 0.75 -3.4 4,439,009 + ytfL b4218 0.83 -1.9 4,439,049 - ytfL b4218 0.86 -0.2 4,439,071 - ytfL b4218 0.88 -0.2 4,439,201 - +0.0 4,439,208 + +0.9 4,439,212 + +1.2 4,439,212 + +0.0 4,439,226 - +0.0 4,439,345 + +0.7 4,439,345 + -3.1 4,439,355 - +0.0 4,439,370 - -4.6 4,439,431 - +0.2 4,439,431 - +0.7 4,439,466 - +0.6 4,439,688 + msrA b4219 0.20 -0.4 4,439,739 + msrA b4219 0.28 -1.9 4,439,759 + msrA b4219 0.31 -2.2 4,439,759 + msrA b4219 0.31 -2.0 4,439,764 + msrA b4219 0.32 -0.1 4,439,872 + msrA b4219 0.49 -1.2 4,439,937 - msrA b4219 0.59 -1.1 4,439,937 - msrA b4219 0.59 -1.8 4,440,009 + msrA b4219 0.70 +0.7 4,440,090 + msrA b4219 0.83 +0.6 4,440,120 - msrA b4219 0.87 +0.6 4,440,131 - msrA b4219 0.89 +0.3 4,440,143 - -0.5 4,440,143 - -0.5 4,440,279 - +0.0 4,440,331 + +0.5 4,440,331 + -2.5 4,440,430 + +1.3 4,440,435 + +0.5 4,440,435 + +0.6 4,440,435 + +0.7 4,440,483 - -0.6 4,440,483 - +0.9 4,440,485 - -1.4 4,440,582 + ytfM b4220 0.10 +0.4 4,440,582 - ytfM b4220 0.10 -0.5 4,440,675 - ytfM b4220 0.16 -1.0 4,440,681 + ytfM b4220 0.16 +2.8 4,440,698 + ytfM b4220 0.17 +0.2 4,440,740 + ytfM b4220 0.19 +0.6 4,440,741 - ytfM b4220 0.19 -3.9 4,440,741 - ytfM b4220 0.19 -4.0 4,440,770 - ytfM b4220 0.21 +1.3 4,440,770 - ytfM b4220 0.21 -0.1 4,440,798 + ytfM b4220 0.23 -3.7 4,440,810 + ytfM b4220 0.23 +1.3 4,440,810 + ytfM b4220 0.23 +1.4 4,440,810 + ytfM b4220 0.23 +1.1 4,440,810 + ytfM b4220 0.23 +0.8 4,440,810 + ytfM b4220 0.23 -1.1 4,440,818 - ytfM b4220 0.24 -4.4 4,440,861 + ytfM b4220 0.26 +0.2 4,440,864 - ytfM b4220 0.26 +0.2 4,440,864 - ytfM b4220 0.26 +1.4 4,440,935 + ytfM b4220 0.31 -0.5 4,440,935 + ytfM b4220 0.31 +1.1 4,440,948 + ytfM b4220 0.31 +2.0 4,440,973 + ytfM b4220 0.33 -0.6 4,440,976 - ytfM b4220 0.33 -0.5 4,440,989 + ytfM b4220 0.34 +0.2 4,440,996 - ytfM b4220 0.34 -0.5 4,441,096 + ytfM b4220 0.40 -1.3 4,441,144 - ytfM b4220 0.43 +1.1
Or see this region's nucleotide sequence