Strain Fitness in Escherichia coli BW25113 around b4195

Experiment: carbon source D-Mannose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsgaT and ulaB are separated by 15 nucleotidesulaB and ulaC are separated by 9 nucleotidesulaC and ulaD are separated by 13 nucleotidesulaD and ulaE are separated by 9 nucleotides b4193: sgaT - orf, hypothetical protein (VIMSS), at 4,418,003 to 4,419,400 sgaT b4194: ulaB - L-ascorbate-specific enzyme IIB component of PTS (NCBI), at 4,419,416 to 4,419,721 ulaB b4195: ulaC - L-ascorbate-specific enzyme IIA component of PTS (NCBI), at 4,419,731 to 4,420,195 ulaC b4196: ulaD - 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI), at 4,420,209 to 4,420,859 ulaD b4197: ulaE - L-xylulose 5-phosphate 3-epimerase (NCBI), at 4,420,869 to 4,421,723 ulaE Position (kb) 4419 4420 4421Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4418.736 kb on + strand, within sgaTat 4418.776 kb on - strand, within sgaTat 4418.787 kb on - strand, within sgaTat 4418.787 kb on - strand, within sgaTat 4418.798 kb on + strand, within sgaTat 4418.801 kb on + strand, within sgaTat 4418.801 kb on + strand, within sgaTat 4418.801 kb on + strand, within sgaTat 4418.801 kb on + strand, within sgaTat 4418.809 kb on - strand, within sgaTat 4418.809 kb on - strand, within sgaTat 4418.882 kb on - strand, within sgaTat 4418.914 kb on + strand, within sgaTat 4418.946 kb on + strand, within sgaTat 4419.069 kb on + strand, within sgaTat 4419.069 kb on + strand, within sgaTat 4419.148 kb on - strand, within sgaTat 4419.170 kb on + strand, within sgaTat 4419.245 kb on + strand, within sgaTat 4419.291 kb on + strandat 4419.357 kb on + strandat 4419.357 kb on + strandat 4419.431 kb on - strandat 4419.457 kb on - strand, within ulaBat 4419.584 kb on + strand, within ulaBat 4419.584 kb on + strand, within ulaBat 4419.584 kb on + strand, within ulaBat 4419.630 kb on + strand, within ulaBat 4419.869 kb on + strand, within ulaCat 4419.869 kb on + strand, within ulaCat 4419.924 kb on - strand, within ulaCat 4419.929 kb on - strand, within ulaCat 4419.947 kb on - strand, within ulaCat 4420.137 kb on + strand, within ulaCat 4420.164 kb on + strandat 4420.164 kb on + strandat 4420.206 kb on + strandat 4420.355 kb on - strand, within ulaDat 4420.363 kb on + strand, within ulaDat 4420.386 kb on - strand, within ulaDat 4420.479 kb on + strand, within ulaDat 4420.533 kb on + strand, within ulaDat 4420.641 kb on + strand, within ulaDat 4420.719 kb on + strand, within ulaDat 4420.719 kb on + strand, within ulaDat 4420.769 kb on + strand, within ulaDat 4420.848 kb on - strandat 4420.898 kb on + strandat 4420.898 kb on + strandat 4421.038 kb on + strand, within ulaEat 4421.046 kb on - strand, within ulaEat 4421.046 kb on - strand, within ulaEat 4421.148 kb on - strand, within ulaEat 4421.156 kb on + strand, within ulaE

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM
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4,418,736 + sgaT b4193 0.52 -0.5
4,418,776 - sgaT b4193 0.55 +0.4
4,418,787 - sgaT b4193 0.56 -0.6
4,418,787 - sgaT b4193 0.56 -0.9
4,418,798 + sgaT b4193 0.57 -1.2
4,418,801 + sgaT b4193 0.57 +0.1
4,418,801 + sgaT b4193 0.57 -1.0
4,418,801 + sgaT b4193 0.57 -3.5
4,418,801 + sgaT b4193 0.57 -1.0
4,418,809 - sgaT b4193 0.58 -0.2
4,418,809 - sgaT b4193 0.58 +0.3
4,418,882 - sgaT b4193 0.63 +0.2
4,418,914 + sgaT b4193 0.65 +0.7
4,418,946 + sgaT b4193 0.67 -1.8
4,419,069 + sgaT b4193 0.76 -0.6
4,419,069 + sgaT b4193 0.76 +1.1
4,419,148 - sgaT b4193 0.82 -0.2
4,419,170 + sgaT b4193 0.83 +0.3
4,419,245 + sgaT b4193 0.89 -1.2
4,419,291 + -1.5
4,419,357 + +0.2
4,419,357 + -4.1
4,419,431 - -0.1
4,419,457 - ulaB b4194 0.13 -0.1
4,419,584 + ulaB b4194 0.55 -1.0
4,419,584 + ulaB b4194 0.55 -0.1
4,419,584 + ulaB b4194 0.55 -0.3
4,419,630 + ulaB b4194 0.70 -1.3
4,419,869 + ulaC b4195 0.30 +1.3
4,419,869 + ulaC b4195 0.30 +0.2
4,419,924 - ulaC b4195 0.42 +0.9
4,419,929 - ulaC b4195 0.43 +0.5
4,419,947 - ulaC b4195 0.46 +1.1
4,420,137 + ulaC b4195 0.87 -4.2
4,420,164 + +1.4
4,420,164 + -2.0
4,420,206 + -0.8
4,420,355 - ulaD b4196 0.22 +1.5
4,420,363 + ulaD b4196 0.24 +1.2
4,420,386 - ulaD b4196 0.27 +1.5
4,420,479 + ulaD b4196 0.41 -0.4
4,420,533 + ulaD b4196 0.50 +0.7
4,420,641 + ulaD b4196 0.66 -3.5
4,420,719 + ulaD b4196 0.78 -0.9
4,420,719 + ulaD b4196 0.78 -1.7
4,420,769 + ulaD b4196 0.86 -0.2
4,420,848 - -2.2
4,420,898 + -0.4
4,420,898 + +1.5
4,421,038 + ulaE b4197 0.20 -0.2
4,421,046 - ulaE b4197 0.21 -0.4
4,421,046 - ulaE b4197 0.21 -1.6
4,421,148 - ulaE b4197 0.33 -1.1
4,421,156 + ulaE b4197 0.34 +0.2

Or see this region's nucleotide sequence