Strain Fitness in Escherichia coli BW25113 around b3999

Experiment: carbon source D-Mannose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthemE and nfi are separated by 9 nucleotidesnfi and yjaG are separated by 42 nucleotidesyjaG and hupA are separated by 186 nucleotideshupA and yjaH are separated by 12 nucleotides b3997: hemE - uroporphyrinogen decarboxylase (NCBI), at 4,195,739 to 4,196,803 hemE b3998: nfi - endonuclease V (deoxyinosine 3'endoduclease) (VIMSS), at 4,196,813 to 4,197,484 nfi b3999: yjaG - hypothetical protein (NCBI), at 4,197,527 to 4,198,117 yjaG b4000: hupA - HU, DNA-binding transcriptional regulator, alpha subunit (NCBI), at 4,198,304 to 4,198,576 hupA b4001: yjaH - hypothetical protein (NCBI), at 4,198,589 to 4,199,284 yjaH Position (kb) 4197 4198 4199Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 4196.940 kb on - strand, within nfiat 4196.973 kb on - strand, within nfiat 4197.077 kb on + strand, within nfiat 4197.155 kb on - strand, within nfiat 4197.274 kb on - strand, within nfiat 4197.274 kb on - strand, within nfiat 4197.291 kb on + strand, within nfiat 4197.291 kb on + strand, within nfiat 4197.316 kb on + strand, within nfiat 4197.331 kb on + strand, within nfiat 4197.353 kb on + strand, within nfiat 4197.353 kb on + strand, within nfiat 4197.353 kb on + strand, within nfiat 4197.361 kb on - strand, within nfiat 4197.361 kb on - strand, within nfiat 4197.383 kb on - strand, within nfiat 4197.383 kb on - strand, within nfiat 4197.436 kb on - strandat 4197.436 kb on - strandat 4197.471 kb on - strandat 4197.593 kb on - strand, within yjaGat 4197.608 kb on + strand, within yjaGat 4197.618 kb on - strand, within yjaGat 4197.618 kb on - strand, within yjaGat 4197.733 kb on - strand, within yjaGat 4197.855 kb on - strand, within yjaGat 4197.855 kb on - strand, within yjaGat 4197.871 kb on + strand, within yjaGat 4197.879 kb on - strand, within yjaGat 4197.911 kb on + strand, within yjaGat 4197.911 kb on + strand, within yjaGat 4197.983 kb on - strand, within yjaGat 4198.028 kb on + strand, within yjaGat 4198.028 kb on + strand, within yjaGat 4198.070 kb on - strandat 4198.162 kb on + strandat 4198.235 kb on - strandat 4198.245 kb on - strandat 4198.250 kb on - strandat 4198.266 kb on + strandat 4198.287 kb on - strandat 4198.287 kb on - strandat 4198.359 kb on - strand, within hupAat 4198.359 kb on - strand, within hupAat 4198.380 kb on + strand, within hupAat 4198.380 kb on + strand, within hupAat 4198.388 kb on - strand, within hupAat 4198.409 kb on + strand, within hupAat 4198.409 kb on + strand, within hupAat 4198.425 kb on + strand, within hupAat 4198.443 kb on - strand, within hupAat 4198.457 kb on - strand, within hupAat 4198.577 kb on - strandat 4198.579 kb on - strandat 4198.579 kb on - strandat 4198.581 kb on + strandat 4198.581 kb on + strandat 4198.824 kb on + strand, within yjaHat 4198.824 kb on + strand, within yjaHat 4198.833 kb on + strand, within yjaHat 4198.833 kb on + strand, within yjaHat 4198.925 kb on + strand, within yjaHat 4198.932 kb on + strand, within yjaHat 4198.996 kb on + strand, within yjaHat 4199.081 kb on - strand, within yjaH

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM
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4,196,940 - nfi b3998 0.19 +0.2
4,196,973 - nfi b3998 0.24 -1.9
4,197,077 + nfi b3998 0.39 -1.1
4,197,155 - nfi b3998 0.51 +1.7
4,197,274 - nfi b3998 0.69 -1.2
4,197,274 - nfi b3998 0.69 +0.4
4,197,291 + nfi b3998 0.71 +1.4
4,197,291 + nfi b3998 0.71 -0.2
4,197,316 + nfi b3998 0.75 -0.1
4,197,331 + nfi b3998 0.77 +0.5
4,197,353 + nfi b3998 0.80 +0.1
4,197,353 + nfi b3998 0.80 +0.1
4,197,353 + nfi b3998 0.80 +0.6
4,197,361 - nfi b3998 0.82 -4.6
4,197,361 - nfi b3998 0.82 +0.9
4,197,383 - nfi b3998 0.85 -1.8
4,197,383 - nfi b3998 0.85 +0.7
4,197,436 - +0.2
4,197,436 - +0.8
4,197,471 - +0.6
4,197,593 - yjaG b3999 0.11 +0.3
4,197,608 + yjaG b3999 0.14 -0.4
4,197,618 - yjaG b3999 0.15 -2.0
4,197,618 - yjaG b3999 0.15 +2.9
4,197,733 - yjaG b3999 0.35 +0.9
4,197,855 - yjaG b3999 0.55 -1.7
4,197,855 - yjaG b3999 0.55 +0.3
4,197,871 + yjaG b3999 0.58 +0.5
4,197,879 - yjaG b3999 0.60 -2.9
4,197,911 + yjaG b3999 0.65 -0.2
4,197,911 + yjaG b3999 0.65 +1.7
4,197,983 - yjaG b3999 0.77 +1.5
4,198,028 + yjaG b3999 0.85 +2.1
4,198,028 + yjaG b3999 0.85 +2.3
4,198,070 - +2.4
4,198,162 + -0.2
4,198,235 - +2.1
4,198,245 - +0.0
4,198,250 - -3.4
4,198,266 + -0.7
4,198,287 - +0.1
4,198,287 - -0.5
4,198,359 - hupA b4000 0.20 -3.5
4,198,359 - hupA b4000 0.20 -3.9
4,198,380 + hupA b4000 0.28 -3.3
4,198,380 + hupA b4000 0.28 -1.3
4,198,388 - hupA b4000 0.31 +0.0
4,198,409 + hupA b4000 0.38 -5.6
4,198,409 + hupA b4000 0.38 -4.5
4,198,425 + hupA b4000 0.44 -1.4
4,198,443 - hupA b4000 0.51 +1.0
4,198,457 - hupA b4000 0.56 -3.8
4,198,577 - -1.6
4,198,579 - -0.6
4,198,579 - -0.1
4,198,581 + -4.0
4,198,581 + +0.9
4,198,824 + yjaH b4001 0.34 +0.6
4,198,824 + yjaH b4001 0.34 -1.7
4,198,833 + yjaH b4001 0.35 +1.4
4,198,833 + yjaH b4001 0.35 +1.0
4,198,925 + yjaH b4001 0.48 +0.1
4,198,932 + yjaH b4001 0.49 +0.1
4,198,996 + yjaH b4001 0.58 +0.8
4,199,081 - yjaH b4001 0.71 -0.4

Or see this region's nucleotide sequence