Experiment: carbon source D-Mannose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdsD and kdsC are separated by 20 nucleotides kdsC and yrbK overlap by 4 nucleotides yrbK and yhbN overlap by 32 nucleotides yhbN and yhbG are separated by 6 nucleotides
b3197: kdsD - D-arabinose 5-phosphate isomerase (NCBI), at 3,339,288 to 3,340,274
kdsD
b3198: kdsC - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI), at 3,340,295 to 3,340,861
kdsC
b3199: yrbK - hypothetical protein (NCBI), at 3,340,858 to 3,341,433
yrbK
b3200: yhbN - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 3,341,402 to 3,341,959
yhbN
b3201: yhbG - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 3,341,966 to 3,342,691
yhbG
Position (kb)
3340
3341
3342 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3339.864 kb on + strand, within kdsD at 3339.868 kb on - strand, within kdsD at 3339.876 kb on + strand, within kdsD at 3339.879 kb on - strand, within kdsD at 3339.879 kb on - strand, within kdsD at 3339.924 kb on + strand, within kdsD at 3339.974 kb on + strand, within kdsD at 3340.032 kb on + strand, within kdsD at 3340.105 kb on - strand, within kdsD at 3340.129 kb on + strand, within kdsD at 3340.129 kb on + strand, within kdsD at 3340.203 kb on + strand at 3340.203 kb on + strand at 3340.231 kb on + strand at 3340.269 kb on + strand at 3340.326 kb on + strand at 3340.326 kb on + strand at 3340.326 kb on + strand at 3340.374 kb on + strand, within kdsC at 3340.374 kb on + strand, within kdsC at 3340.391 kb on + strand, within kdsC at 3340.391 kb on + strand, within kdsC at 3340.736 kb on + strand, within kdsC at 3341.218 kb on + strand, within yrbK at 3341.218 kb on + strand, within yrbK at 3341.256 kb on + strand, within yrbK at 3341.256 kb on + strand, within yrbK at 3341.388 kb on + strand at 3341.388 kb on + strand at 3341.944 kb on + strand at 3341.944 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM remove 3,339,864 + kdsD b3197 0.58 -4.8 3,339,868 - kdsD b3197 0.59 +0.9 3,339,876 + kdsD b3197 0.60 +0.4 3,339,879 - kdsD b3197 0.60 +1.0 3,339,879 - kdsD b3197 0.60 -4.1 3,339,924 + kdsD b3197 0.64 +0.5 3,339,974 + kdsD b3197 0.70 +1.7 3,340,032 + kdsD b3197 0.75 +0.4 3,340,105 - kdsD b3197 0.83 -1.4 3,340,129 + kdsD b3197 0.85 +1.0 3,340,129 + kdsD b3197 0.85 +0.1 3,340,203 + +1.8 3,340,203 + -0.2 3,340,231 + +0.2 3,340,269 + +0.9 3,340,326 + -3.2 3,340,326 + +0.6 3,340,326 + +2.2 3,340,374 + kdsC b3198 0.14 +1.4 3,340,374 + kdsC b3198 0.14 +1.7 3,340,391 + kdsC b3198 0.17 +1.1 3,340,391 + kdsC b3198 0.17 -0.8 3,340,736 + kdsC b3198 0.78 +0.5 3,341,218 + yrbK b3199 0.62 -2.0 3,341,218 + yrbK b3199 0.62 +2.4 3,341,256 + yrbK b3199 0.69 +1.6 3,341,256 + yrbK b3199 0.69 -0.2 3,341,388 + +0.2 3,341,388 + +1.5 3,341,944 + +0.6 3,341,944 + +0.7
Or see this region's nucleotide sequence