Strain Fitness in Escherichia coli BW25113 around b2840

Experiment: carbon source D-Mannose 0.5mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntlysR and ygeA overlap by 14 nucleotidesygeA and araE are separated by 128 nucleotides b2839: lysR - DNA-binding transcriptional dual regulator (NCBI), at 2,977,043 to 2,977,978 lysR b2840: ygeA - predicted racemase (NCBI), at 2,977,965 to 2,978,657 ygeA b2841: araE - arabinose transporter (NCBI), at 2,978,786 to 2,980,204 araE Position (kb) 2977 2978 2979Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2977.048 kb on - strandat 2977.235 kb on - strand, within lysRat 2977.253 kb on + strand, within lysRat 2977.339 kb on - strand, within lysRat 2977.339 kb on - strand, within lysRat 2977.671 kb on - strand, within lysRat 2977.719 kb on + strand, within lysRat 2977.792 kb on - strand, within lysRat 2977.830 kb on + strand, within lysRat 2977.830 kb on - strand, within lysRat 2977.838 kb on + strand, within lysRat 2977.838 kb on + strand, within lysRat 2977.888 kb on + strandat 2977.891 kb on - strandat 2977.891 kb on - strandat 2977.964 kb on + strandat 2978.047 kb on - strand, within ygeAat 2978.150 kb on - strand, within ygeAat 2978.283 kb on - strand, within ygeAat 2978.375 kb on + strand, within ygeAat 2978.407 kb on + strand, within ygeAat 2978.407 kb on + strand, within ygeAat 2978.412 kb on - strand, within ygeAat 2978.412 kb on - strand, within ygeAat 2978.442 kb on + strand, within ygeAat 2978.442 kb on + strand, within ygeAat 2978.530 kb on + strand, within ygeAat 2978.535 kb on + strand, within ygeAat 2978.538 kb on - strand, within ygeAat 2978.554 kb on + strand, within ygeAat 2978.562 kb on - strand, within ygeAat 2978.672 kb on - strandat 2978.705 kb on + strandat 2978.778 kb on - strandat 2978.847 kb on - strandat 2978.893 kb on + strandat 2978.900 kb on + strandat 2978.910 kb on + strandat 2978.918 kb on - strandat 2978.953 kb on + strand, within araEat 2978.953 kb on + strand, within araEat 2978.988 kb on + strand, within araEat 2978.988 kb on + strand, within araEat 2979.068 kb on - strand, within araEat 2979.068 kb on - strand, within araEat 2979.070 kb on - strand, within araEat 2979.123 kb on - strand, within araEat 2979.251 kb on - strand, within araEat 2979.260 kb on - strand, within araEat 2979.260 kb on - strand, within araEat 2979.287 kb on + strand, within araEat 2979.375 kb on + strand, within araEat 2979.375 kb on + strand, within araEat 2979.375 kb on + strand, within araEat 2979.478 kb on + strand, within araEat 2979.478 kb on + strand, within araEat 2979.568 kb on + strand, within araEat 2979.582 kb on + strand, within araEat 2979.622 kb on + strand, within araE

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM
remove
2,977,048 - -0.9
2,977,235 - lysR b2839 0.21 -1.4
2,977,253 + lysR b2839 0.22 -5.7
2,977,339 - lysR b2839 0.32 -4.6
2,977,339 - lysR b2839 0.32 -3.3
2,977,671 - lysR b2839 0.67 -4.0
2,977,719 + lysR b2839 0.72 -5.0
2,977,792 - lysR b2839 0.80 -0.7
2,977,830 + lysR b2839 0.84 -3.9
2,977,830 - lysR b2839 0.84 -5.3
2,977,838 + lysR b2839 0.85 -4.1
2,977,838 + lysR b2839 0.85 -3.3
2,977,888 + -0.7
2,977,891 - -4.5
2,977,891 - -4.6
2,977,964 + +0.6
2,978,047 - ygeA b2840 0.12 +0.5
2,978,150 - ygeA b2840 0.27 -0.3
2,978,283 - ygeA b2840 0.46 +0.5
2,978,375 + ygeA b2840 0.59 -2.0
2,978,407 + ygeA b2840 0.64 -0.9
2,978,407 + ygeA b2840 0.64 +0.1
2,978,412 - ygeA b2840 0.65 -0.8
2,978,412 - ygeA b2840 0.65 -3.1
2,978,442 + ygeA b2840 0.69 -1.4
2,978,442 + ygeA b2840 0.69 +1.0
2,978,530 + ygeA b2840 0.82 -1.9
2,978,535 + ygeA b2840 0.82 -0.1
2,978,538 - ygeA b2840 0.83 -1.9
2,978,554 + ygeA b2840 0.85 -0.5
2,978,562 - ygeA b2840 0.86 +0.5
2,978,672 - +0.1
2,978,705 + -1.3
2,978,778 - +0.8
2,978,847 - -2.2
2,978,893 + +0.4
2,978,900 + -1.4
2,978,910 + +0.5
2,978,918 - -0.4
2,978,953 + araE b2841 0.12 +0.8
2,978,953 + araE b2841 0.12 -2.2
2,978,988 + araE b2841 0.14 +0.5
2,978,988 + araE b2841 0.14 +1.4
2,979,068 - araE b2841 0.20 -0.8
2,979,068 - araE b2841 0.20 +0.9
2,979,070 - araE b2841 0.20 +0.6
2,979,123 - araE b2841 0.24 -0.4
2,979,251 - araE b2841 0.33 +1.3
2,979,260 - araE b2841 0.33 -0.0
2,979,260 - araE b2841 0.33 -0.5
2,979,287 + araE b2841 0.35 +2.5
2,979,375 + araE b2841 0.42 -2.8
2,979,375 + araE b2841 0.42 -1.0
2,979,375 + araE b2841 0.42 +1.6
2,979,478 + araE b2841 0.49 +0.7
2,979,478 + araE b2841 0.49 +0.6
2,979,568 + araE b2841 0.55 +0.1
2,979,582 + araE b2841 0.56 +0.7
2,979,622 + araE b2841 0.59 -4.1

Or see this region's nucleotide sequence