Strain Fitness in Escherichia coli BW25113 around b1943

Experiment: carbon source D-Mannose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliI and fliJ are separated by 18 nucleotidesfliJ and fliK overlap by 4 nucleotidesfliK and fliL are separated by 104 nucleotidesfliL and fliM are separated by 4 nucleotides b1941: fliI - flagellum-specific ATP synthase (NCBI), at 2,014,578 to 2,015,951 fliI b1942: fliJ - flagellar biosynthesis chaperone (NCBI), at 2,015,970 to 2,016,413 fliJ b1943: fliK - flagellar hook-length control protein (NCBI), at 2,016,410 to 2,017,537 fliK b1944: fliL - flagellar basal body-associated protein FliL (NCBI), at 2,017,642 to 2,018,106 fliL b1945: fliM - flagellar motor switch protein M (NCBI), at 2,018,111 to 2,019,115 fliM Position (kb) 2016 2017 2018Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2015.499 kb on - strand, within fliIat 2015.549 kb on + strand, within fliIat 2015.549 kb on + strand, within fliIat 2015.560 kb on + strand, within fliIat 2015.631 kb on + strand, within fliIat 2015.685 kb on - strand, within fliIat 2015.685 kb on - strand, within fliIat 2015.701 kb on + strandat 2015.701 kb on + strand, within fliIat 2015.847 kb on - strandat 2015.847 kb on - strandat 2016.216 kb on - strand, within fliJat 2016.216 kb on - strand, within fliJat 2016.658 kb on - strand, within fliKat 2016.927 kb on - strand, within fliKat 2016.957 kb on - strand, within fliKat 2017.027 kb on + strand, within fliKat 2017.068 kb on - strand, within fliKat 2017.076 kb on + strand, within fliKat 2017.076 kb on + strand, within fliKat 2017.080 kb on - strand, within fliKat 2017.116 kb on + strand, within fliKat 2017.182 kb on + strand, within fliKat 2017.207 kb on + strand, within fliKat 2017.207 kb on + strand, within fliKat 2017.267 kb on + strand, within fliKat 2017.323 kb on + strand, within fliKat 2017.488 kb on + strandat 2017.757 kb on + strand, within fliLat 2017.757 kb on + strand, within fliLat 2017.797 kb on - strand, within fliLat 2017.813 kb on + strand, within fliLat 2017.947 kb on - strand, within fliLat 2017.972 kb on + strand, within fliLat 2018.029 kb on - strand, within fliLat 2018.038 kb on - strand, within fliLat 2018.038 kb on - strand, within fliLat 2018.299 kb on + strand, within fliMat 2018.411 kb on + strand, within fliMat 2018.420 kb on - strand, within fliMat 2018.466 kb on + strand, within fliMat 2018.466 kb on + strand, within fliM

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM
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2,015,499 - fliI b1941 0.67 +0.1
2,015,549 + fliI b1941 0.71 +0.2
2,015,549 + fliI b1941 0.71 +0.7
2,015,560 + fliI b1941 0.71 -0.3
2,015,631 + fliI b1941 0.77 +0.1
2,015,685 - fliI b1941 0.81 +0.1
2,015,685 - fliI b1941 0.81 -4.5
2,015,701 + -1.5
2,015,701 + fliI b1941 0.82 -0.0
2,015,847 - +0.5
2,015,847 - -0.1
2,016,216 - fliJ b1942 0.55 -0.1
2,016,216 - fliJ b1942 0.55 -1.1
2,016,658 - fliK b1943 0.22 +0.0
2,016,927 - fliK b1943 0.46 +0.6
2,016,957 - fliK b1943 0.48 -0.9
2,017,027 + fliK b1943 0.55 +1.9
2,017,068 - fliK b1943 0.58 +1.6
2,017,076 + fliK b1943 0.59 +1.3
2,017,076 + fliK b1943 0.59 +0.5
2,017,080 - fliK b1943 0.59 +0.6
2,017,116 + fliK b1943 0.63 -0.9
2,017,182 + fliK b1943 0.68 +0.5
2,017,207 + fliK b1943 0.71 +1.6
2,017,207 + fliK b1943 0.71 +3.4
2,017,267 + fliK b1943 0.76 +0.5
2,017,323 + fliK b1943 0.81 -0.5
2,017,488 + -0.4
2,017,757 + fliL b1944 0.25 +1.2
2,017,757 + fliL b1944 0.25 -0.4
2,017,797 - fliL b1944 0.33 +0.8
2,017,813 + fliL b1944 0.37 +1.2
2,017,947 - fliL b1944 0.66 -0.5
2,017,972 + fliL b1944 0.71 -0.0
2,018,029 - fliL b1944 0.83 -0.0
2,018,038 - fliL b1944 0.85 +0.2
2,018,038 - fliL b1944 0.85 +0.9
2,018,299 + fliM b1945 0.19 +0.6
2,018,411 + fliM b1945 0.30 +0.2
2,018,420 - fliM b1945 0.31 -1.0
2,018,466 + fliM b1945 0.35 +0.2
2,018,466 + fliM b1945 0.35 -0.7

Or see this region's nucleotide sequence