Strain Fitness in Escherichia coli BW25113 around b1449

Experiment: carbon source D-Mannose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydcZ and yncA overlap by 4 nucleotidesyncA and yncB are separated by 180 nucleotidesyncB and b1450 are separated by 197 nucleotidesb1450 and yncD are separated by 35 nucleotides b1447: ydcZ - predicted inner membrane protein (NCBI), at 1,515,906 to 1,516,355 ydcZ b1448: yncA - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 1,516,352 to 1,516,870 yncA b1449: yncB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq), at 1,517,051 to 1,518,088 yncB b1450: b1450 - orf, hypothetical protein (VIMSS), at 1,518,286 to 1,518,951 b1450 b1451: yncD - predicted iron outer membrane transporter (NCBI), at 1,518,987 to 1,521,089 yncD Position (kb) 1517 1518 1519Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1516.613 kb on - strand, within yncAat 1516.789 kb on - strand, within yncAat 1516.831 kb on + strandat 1516.870 kb on + strandat 1516.878 kb on - strandat 1517.165 kb on + strand, within yncBat 1517.165 kb on + strand, within yncBat 1517.246 kb on - strand, within yncBat 1517.310 kb on + strand, within yncBat 1517.341 kb on + strand, within yncBat 1517.341 kb on + strand, within yncBat 1517.496 kb on - strand, within yncBat 1517.517 kb on - strand, within yncBat 1517.635 kb on - strand, within yncBat 1517.635 kb on - strand, within yncBat 1517.739 kb on - strand, within yncBat 1517.739 kb on - strand, within yncBat 1517.741 kb on - strand, within yncBat 1517.805 kb on + strand, within yncBat 1517.805 kb on + strand, within yncBat 1517.839 kb on - strand, within yncBat 1517.839 kb on - strand, within yncBat 1517.923 kb on + strand, within yncBat 1517.923 kb on + strand, within yncBat 1517.950 kb on - strand, within yncBat 1517.950 kb on - strand, within yncBat 1518.047 kb on - strandat 1518.065 kb on - strandat 1518.067 kb on - strandat 1518.147 kb on + strandat 1518.175 kb on - strandat 1518.175 kb on - strandat 1518.200 kb on + strandat 1518.225 kb on - strandat 1518.237 kb on + strandat 1518.254 kb on + strandat 1518.295 kb on - strandat 1518.330 kb on + strandat 1518.338 kb on - strandat 1518.349 kb on - strandat 1518.354 kb on + strand, within b1450at 1518.423 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.495 kb on + strand, within b1450at 1518.498 kb on + strand, within b1450at 1518.506 kb on + strand, within b1450at 1518.746 kb on - strand, within b1450at 1518.756 kb on + strand, within b1450at 1518.756 kb on + strand, within b1450at 1518.767 kb on + strand, within b1450at 1518.775 kb on - strand, within b1450at 1518.779 kb on + strand, within b1450at 1518.844 kb on + strand, within b1450at 1518.844 kb on + strand, within b1450at 1518.871 kb on + strand, within b1450at 1518.872 kb on + strand, within b1450at 1518.954 kb on - strandat 1518.974 kb on + strandat 1519.053 kb on - strandat 1519.084 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM
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1,516,613 - yncA b1448 0.50 -0.5
1,516,789 - yncA b1448 0.84 +0.5
1,516,831 + +0.4
1,516,870 + -0.1
1,516,878 - -0.9
1,517,165 + yncB b1449 0.11 -2.1
1,517,165 + yncB b1449 0.11 +0.1
1,517,246 - yncB b1449 0.19 +0.4
1,517,310 + yncB b1449 0.25 -0.2
1,517,341 + yncB b1449 0.28 -0.5
1,517,341 + yncB b1449 0.28 -0.5
1,517,496 - yncB b1449 0.43 +0.2
1,517,517 - yncB b1449 0.45 +0.3
1,517,635 - yncB b1449 0.56 +0.1
1,517,635 - yncB b1449 0.56 -1.3
1,517,739 - yncB b1449 0.66 +0.1
1,517,739 - yncB b1449 0.66 -0.3
1,517,741 - yncB b1449 0.66 +0.9
1,517,805 + yncB b1449 0.73 -3.7
1,517,805 + yncB b1449 0.73 -0.7
1,517,839 - yncB b1449 0.76 -0.9
1,517,839 - yncB b1449 0.76 -2.3
1,517,923 + yncB b1449 0.84 -0.3
1,517,923 + yncB b1449 0.84 -0.4
1,517,950 - yncB b1449 0.87 +1.1
1,517,950 - yncB b1449 0.87 +1.4
1,518,047 - -1.4
1,518,065 - +1.9
1,518,067 - +1.9
1,518,147 + -3.0
1,518,175 - -0.9
1,518,175 - -2.5
1,518,200 + +1.8
1,518,225 - -1.9
1,518,237 + -0.8
1,518,254 + -3.4
1,518,295 - -0.3
1,518,330 + +0.8
1,518,338 - -2.1
1,518,349 - -1.1
1,518,354 + b1450 b1450 0.10 -3.0
1,518,423 + b1450 b1450 0.21 -0.1
1,518,425 + b1450 b1450 0.21 +0.4
1,518,425 + b1450 b1450 0.21 -2.0
1,518,425 + b1450 b1450 0.21 +1.5
1,518,425 + b1450 b1450 0.21 +0.8
1,518,495 + b1450 b1450 0.31 -1.0
1,518,498 + b1450 b1450 0.32 -3.5
1,518,506 + b1450 b1450 0.33 -3.3
1,518,746 - b1450 b1450 0.69 +0.4
1,518,756 + b1450 b1450 0.71 +0.9
1,518,756 + b1450 b1450 0.71 +1.5
1,518,767 + b1450 b1450 0.72 -4.4
1,518,775 - b1450 b1450 0.73 +1.9
1,518,779 + b1450 b1450 0.74 +0.8
1,518,844 + b1450 b1450 0.84 +1.7
1,518,844 + b1450 b1450 0.84 +0.9
1,518,871 + b1450 b1450 0.88 +1.4
1,518,872 + b1450 b1450 0.88 +1.7
1,518,954 - -2.3
1,518,974 + -1.4
1,519,053 - +1.1
1,519,084 - -1.1

Or see this region's nucleotide sequence