Strain Fitness in Escherichia coli BW25113 around b1217

Experiment: carbon source D-Mannose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntchaA and chaB are separated by 269 nucleotideschaB and chaC are separated by 157 nucleotideschaC and ychN are separated by 43 nucleotides b1216: chaA - calcium/sodium:proton antiporter (NCBI), at 1,269,972 to 1,271,072 chaA b1217: chaB - cation transport regulator (NCBI), at 1,271,342 to 1,271,572 chaB b1218: chaC - regulatory protein for cation transport (RefSeq), at 1,271,730 to 1,272,425 chaC b1219: ychN - hypothetical protein (NCBI), at 1,272,469 to 1,272,822 ychN Position (kb) 1271 1272Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1270.415 kb on - strand, within chaAat 1270.552 kb on + strand, within chaAat 1270.588 kb on + strand, within chaAat 1270.615 kb on + strand, within chaAat 1270.622 kb on - strand, within chaAat 1270.637 kb on + strand, within chaAat 1270.639 kb on + strand, within chaAat 1270.704 kb on - strand, within chaAat 1270.704 kb on - strand, within chaAat 1270.750 kb on - strand, within chaAat 1270.755 kb on - strand, within chaAat 1270.780 kb on - strand, within chaAat 1270.790 kb on - strand, within chaAat 1270.790 kb on - strand, within chaAat 1270.878 kb on + strand, within chaAat 1270.878 kb on + strand, within chaAat 1270.972 kb on - strandat 1270.972 kb on - strandat 1270.977 kb on - strandat 1270.986 kb on + strandat 1270.988 kb on + strandat 1270.994 kb on + strandat 1270.994 kb on + strandat 1271.045 kb on - strandat 1271.054 kb on + strandat 1271.074 kb on + strandat 1271.074 kb on + strandat 1271.083 kb on - strandat 1271.083 kb on - strandat 1271.145 kb on + strandat 1271.248 kb on + strandat 1271.254 kb on - strandat 1271.256 kb on - strandat 1271.342 kb on - strandat 1271.347 kb on + strandat 1271.381 kb on + strand, within chaBat 1271.381 kb on + strand, within chaBat 1271.475 kb on - strand, within chaBat 1271.723 kb on + strandat 1271.892 kb on + strand, within chaCat 1271.892 kb on + strand, within chaCat 1271.932 kb on - strand, within chaCat 1272.143 kb on - strand, within chaCat 1272.159 kb on - strand, within chaCat 1272.288 kb on - strand, within chaCat 1272.522 kb on - strand, within ychNat 1272.532 kb on - strand, within ychN

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM
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1,270,415 - chaA b1216 0.40 -1.0
1,270,552 + chaA b1216 0.53 +0.6
1,270,588 + chaA b1216 0.56 +1.2
1,270,615 + chaA b1216 0.58 -0.6
1,270,622 - chaA b1216 0.59 +0.1
1,270,637 + chaA b1216 0.60 -0.9
1,270,639 + chaA b1216 0.61 +0.3
1,270,704 - chaA b1216 0.66 -2.2
1,270,704 - chaA b1216 0.66 -1.0
1,270,750 - chaA b1216 0.71 +1.2
1,270,755 - chaA b1216 0.71 +1.7
1,270,780 - chaA b1216 0.73 +0.3
1,270,790 - chaA b1216 0.74 -1.9
1,270,790 - chaA b1216 0.74 -0.2
1,270,878 + chaA b1216 0.82 +1.9
1,270,878 + chaA b1216 0.82 -1.8
1,270,972 - -2.0
1,270,972 - -0.0
1,270,977 - +0.9
1,270,986 + -0.3
1,270,988 + -0.3
1,270,994 + -2.1
1,270,994 + -5.1
1,271,045 - -1.0
1,271,054 + +0.8
1,271,074 + +0.7
1,271,074 + +0.0
1,271,083 - -1.5
1,271,083 - +1.2
1,271,145 + +0.2
1,271,248 + -0.1
1,271,254 - +1.3
1,271,256 - +0.2
1,271,342 - -2.9
1,271,347 + +0.3
1,271,381 + chaB b1217 0.17 +1.0
1,271,381 + chaB b1217 0.17 +0.5
1,271,475 - chaB b1217 0.58 +1.2
1,271,723 + +0.1
1,271,892 + chaC b1218 0.23 -0.3
1,271,892 + chaC b1218 0.23 +0.6
1,271,932 - chaC b1218 0.29 -2.0
1,272,143 - chaC b1218 0.59 -0.9
1,272,159 - chaC b1218 0.62 -1.4
1,272,288 - chaC b1218 0.80 -0.3
1,272,522 - ychN b1219 0.15 -0.0
1,272,532 - ychN b1219 0.18 +0.2

Or see this region's nucleotide sequence