Experiment: P. putida PUT10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0272 and PP_0273 are separated by 65 nucleotides PP_0273 and PP_5435 are separated by 175 nucleotides PP_5435 and PP_0274 are separated by 41 nucleotides PP_0274 and PP_0275 are separated by 419 nucleotides
PP_0272: PP_0272 - Outer membrane ferric siderophore receptor, at 330,111 to 332,408
_0272
PP_0273: PP_0273 - conserved protein of unknown function, at 332,474 to 332,686
_0273
PP_5435: PP_5435 - conserved protein of unknown function, at 332,862 to 333,215
_5435
PP_0274: PP_0274 - putative transcriptional regulator containing HTH domain, at 333,257 to 333,730
_0274
PP_0275: PP_0275 - conserved protein of unknown function, at 334,150 to 334,365
_0275
Position (kb) 332
333
334 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 331.932 kb on + strand, within PP_0272 at 331.933 kb on - strand, within PP_0272 at 331.964 kb on + strand, within PP_0272 at 332.026 kb on - strand, within PP_0272 at 332.026 kb on - strand, within PP_0272 at 332.026 kb on - strand, within PP_0272 at 332.097 kb on + strand, within PP_0272 at 332.192 kb on + strand at 332.192 kb on + strand at 332.192 kb on + strand at 332.193 kb on - strand at 332.196 kb on - strand at 332.265 kb on + strand at 332.265 kb on + strand at 332.266 kb on - strand at 332.325 kb on + strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.328 kb on + strand at 332.328 kb on + strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.346 kb on + strand at 332.346 kb on + strand at 332.347 kb on - strand at 332.347 kb on - strand at 332.376 kb on - strand at 332.475 kb on + strand at 332.476 kb on - strand at 332.487 kb on - strand at 332.616 kb on + strand, within PP_0273 at 332.616 kb on + strand, within PP_0273 at 332.616 kb on + strand, within PP_0273 at 332.617 kb on - strand, within PP_0273 at 332.617 kb on - strand, within PP_0273 at 332.715 kb on + strand at 332.715 kb on + strand at 332.778 kb on + strand at 332.779 kb on - strand at 332.795 kb on + strand at 332.796 kb on - strand at 332.837 kb on + strand at 332.837 kb on + strand at 332.837 kb on + strand at 332.837 kb on + strand at 332.837 kb on + strand at 332.838 kb on - strand at 332.838 kb on - strand at 332.838 kb on - strand at 332.838 kb on - strand at 332.838 kb on - strand at 332.929 kb on + strand, within PP_5435 at 333.081 kb on - strand, within PP_5435 at 333.081 kb on - strand, within PP_5435 at 333.081 kb on - strand, within PP_5435 at 333.081 kb on - strand, within PP_5435 at 333.084 kb on + strand, within PP_5435 at 333.084 kb on + strand, within PP_5435 at 333.084 kb on + strand, within PP_5435 at 333.084 kb on + strand, within PP_5435 at 333.085 kb on - strand, within PP_5435 at 333.085 kb on - strand, within PP_5435 at 333.085 kb on - strand, within PP_5435 at 333.146 kb on + strand, within PP_5435 at 333.153 kb on + strand, within PP_5435 at 333.153 kb on + strand, within PP_5435 at 333.154 kb on - strand, within PP_5435 at 333.522 kb on + strand, within PP_0274 at 333.522 kb on + strand, within PP_0274 at 333.830 kb on - strand at 333.864 kb on - strand at 333.940 kb on + strand at 333.940 kb on + strand at 333.940 kb on + strand at 334.004 kb on + strand at 334.020 kb on + strand at 334.085 kb on - strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.117 kb on + strand at 334.117 kb on + strand at 334.118 kb on - strand at 334.118 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1 remove 331,932 + PP_0272 0.79 +0.4 331,933 - PP_0272 0.79 -2.5 331,964 + PP_0272 0.81 +0.5 332,026 - PP_0272 0.83 +0.7 332,026 - PP_0272 0.83 +0.9 332,026 - PP_0272 0.83 +0.4 332,097 + PP_0272 0.86 -1.7 332,192 + -1.4 332,192 + +0.4 332,192 + +0.7 332,193 - -0.1 332,196 - -0.7 332,265 + +0.7 332,265 + -0.2 332,266 - -0.1 332,325 + +1.1 332,326 - -1.1 332,326 - +0.7 332,326 - +0.1 332,326 - +0.1 332,326 - -0.4 332,326 - +0.8 332,326 - -1.5 332,328 + +0.3 332,328 + +0.7 332,329 - -1.8 332,329 - -0.5 332,329 - -0.0 332,329 - +0.2 332,329 - -2.8 332,329 - -0.6 332,346 + +0.3 332,346 + -0.8 332,347 - -2.7 332,347 - -0.8 332,376 - +0.0 332,475 + +0.1 332,476 - +2.1 332,487 - -0.2 332,616 + PP_0273 0.67 -0.4 332,616 + PP_0273 0.67 +1.5 332,616 + PP_0273 0.67 -0.1 332,617 - PP_0273 0.67 -0.5 332,617 - PP_0273 0.67 +1.3 332,715 + +0.0 332,715 + +0.7 332,778 + +0.2 332,779 - +1.6 332,795 + -0.3 332,796 - +0.9 332,837 + -0.1 332,837 + +0.8 332,837 + +1.3 332,837 + -0.2 332,837 + -0.2 332,838 - -0.5 332,838 - +0.4 332,838 - +0.8 332,838 - +0.6 332,838 - +0.7 332,929 + PP_5435 0.19 -0.2 333,081 - PP_5435 0.62 -0.8 333,081 - PP_5435 0.62 -0.8 333,081 - PP_5435 0.62 -1.2 333,081 - PP_5435 0.62 -1.0 333,084 + PP_5435 0.63 -1.7 333,084 + PP_5435 0.63 -0.7 333,084 + PP_5435 0.63 -0.7 333,084 + PP_5435 0.63 -0.5 333,085 - PP_5435 0.63 -0.2 333,085 - PP_5435 0.63 +0.1 333,085 - PP_5435 0.63 -0.8 333,146 + PP_5435 0.80 +0.1 333,153 + PP_5435 0.82 -0.6 333,153 + PP_5435 0.82 +0.3 333,154 - PP_5435 0.82 -1.4 333,522 + PP_0274 0.56 -0.3 333,522 + PP_0274 0.56 +0.6 333,830 - -0.9 333,864 - -0.1 333,940 + -0.0 333,940 + -0.2 333,940 + +0.1 334,004 + +0.3 334,020 + -0.6 334,085 - -1.7 334,112 + -0.9 334,112 + -0.8 334,112 + +0.1 334,112 + +1.3 334,112 + +0.3 334,112 + +0.1 334,112 + +0.2 334,113 - +1.3 334,113 - +0.4 334,113 - +0.6 334,113 - +0.3 334,117 + -0.1 334,117 + -0.1 334,118 - -0.6 334,118 - +0.3
Or see this region's nucleotide sequence