Experiment: P. putida PUT10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2463 and PP_5525 are separated by 162 nucleotides PP_5525 and PP_2465 are separated by 338 nucleotides PP_2465 and PP_2466 are separated by 17 nucleotides PP_2466 and PP_2467 are separated by 45 nucleotides PP_2467 and PP_2468 are separated by 27 nucleotides
PP_2463: PP_2463 - nucleic acid cold-shock chaperone, at 2,808,575 to 2,808,787
_2463
PP_5525: PP_5525 - conserved exported protein of unknown function, at 2,808,950 to 2,809,255
_5525
PP_2465: PP_2465 - Threonine--tRNA ligase, at 2,809,594 to 2,811,516
_2465
PP_2466: PP_2466 - Translation initiation factor IF-3, at 2,811,534 to 2,812,067
_2466
PP_2467: PP_2467 - 50S ribosomal subunit protein L35, at 2,812,113 to 2,812,322
_2467
PP_2468: PP_2468 - 50S ribosomal protein L20, at 2,812,350 to 2,812,706
_2468
Position (kb)
2809
2810
2811
2812 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2808.693 kb on - strand, within PP_2463 at 2808.808 kb on + strand at 2808.809 kb on - strand at 2808.866 kb on + strand at 2808.880 kb on + strand at 2808.880 kb on + strand at 2808.890 kb on + strand at 2808.891 kb on - strand at 2808.944 kb on - strand at 2809.155 kb on + strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.263 kb on + strand at 2809.264 kb on - strand at 2809.292 kb on + strand at 2809.292 kb on + strand at 2809.293 kb on - strand at 2809.332 kb on - strand at 2809.332 kb on - strand at 2809.389 kb on + strand at 2809.389 kb on + strand at 2809.430 kb on + strand at 2809.533 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1 remove 2,808,693 - PP_2463 0.55 +2.6 2,808,808 + +1.9 2,808,809 - +2.1 2,808,866 + +1.5 2,808,880 + +2.4 2,808,880 + +3.3 2,808,890 + +1.8 2,808,891 - +0.6 2,808,944 - +0.1 2,809,155 + PP_5525 0.67 +1.6 2,809,156 - PP_5525 0.67 +0.7 2,809,156 - PP_5525 0.67 -2.8 2,809,156 - PP_5525 0.67 +0.3 2,809,156 - PP_5525 0.67 +0.6 2,809,156 - PP_5525 0.67 +0.5 2,809,263 + +1.0 2,809,264 - +3.0 2,809,292 + -0.8 2,809,292 + +1.5 2,809,293 - +0.9 2,809,332 - -0.9 2,809,332 - +1.7 2,809,389 + +1.0 2,809,389 + +0.8 2,809,430 + +1.2 2,809,533 + +1.9 2,812,095 + +0.9 2,812,095 + -2.2 2,812,095 + -0.4 2,812,095 + -1.5 2,812,095 + +0.4 2,812,095 + +0.7
Or see this region's nucleotide sequence