Experiment: P. putida PUT10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2144 and PP_2145 are separated by 177 nucleotides PP_2145 and PP_2146 are separated by 142 nucleotides
PP_2144: PP_2144 - Transcriptional regulator, TetR family, at 2,445,727 to 2,446,434
_2144
PP_2145: PP_2145 - beta-N-acetylglucosaminidase, at 2,446,612 to 2,447,622
_2145
PP_2146: PP_2146 - helicase SNF2/RAD54 family, at 2,447,765 to 2,451,082
_2146
Position (kb)
2446
2447
2448 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2445.682 kb on + strand at 2445.713 kb on + strand at 2445.723 kb on - strand at 2445.819 kb on + strand, within PP_2144 at 2445.819 kb on + strand, within PP_2144 at 2446.005 kb on - strand, within PP_2144 at 2446.005 kb on - strand, within PP_2144 at 2446.109 kb on - strand, within PP_2144 at 2446.123 kb on + strand, within PP_2144 at 2446.123 kb on + strand, within PP_2144 at 2446.123 kb on + strand at 2446.124 kb on - strand, within PP_2144 at 2446.124 kb on - strand, within PP_2144 at 2446.124 kb on - strand, within PP_2144 at 2446.124 kb on - strand, within PP_2144 at 2446.124 kb on - strand, within PP_2144 at 2446.144 kb on + strand, within PP_2144 at 2446.523 kb on + strand at 2446.524 kb on - strand at 2446.524 kb on - strand at 2446.657 kb on - strand at 2446.657 kb on - strand at 2446.732 kb on - strand, within PP_2145 at 2446.826 kb on + strand, within PP_2145 at 2446.827 kb on - strand, within PP_2145 at 2447.257 kb on + strand, within PP_2145 at 2447.258 kb on - strand, within PP_2145 at 2447.258 kb on - strand, within PP_2145 at 2447.258 kb on - strand, within PP_2145 at 2447.258 kb on - strand, within PP_2145 at 2447.434 kb on - strand, within PP_2145 at 2447.658 kb on - strand at 2447.668 kb on - strand at 2447.744 kb on + strand at 2447.744 kb on + strand at 2447.745 kb on - strand at 2447.745 kb on - strand at 2447.967 kb on + strand at 2448.056 kb on + strand at 2448.459 kb on + strand, within PP_2146 at 2448.459 kb on + strand, within PP_2146 at 2448.460 kb on - strand, within PP_2146 at 2448.460 kb on - strand, within PP_2146 at 2448.460 kb on - strand, within PP_2146 at 2448.460 kb on - strand, within PP_2146 at 2448.463 kb on + strand, within PP_2146 at 2448.463 kb on + strand, within PP_2146
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1 remove 2,445,682 + -0.9 2,445,713 + -0.6 2,445,723 - +1.2 2,445,819 + PP_2144 0.13 +1.6 2,445,819 + PP_2144 0.13 +1.8 2,446,005 - PP_2144 0.39 +1.4 2,446,005 - PP_2144 0.39 +1.1 2,446,109 - PP_2144 0.54 -0.2 2,446,123 + PP_2144 0.56 +2.4 2,446,123 + PP_2144 0.56 +1.7 2,446,123 + +0.6 2,446,124 - PP_2144 0.56 +2.8 2,446,124 - PP_2144 0.56 -0.7 2,446,124 - PP_2144 0.56 +1.7 2,446,124 - PP_2144 0.56 +0.9 2,446,124 - PP_2144 0.56 +2.4 2,446,144 + PP_2144 0.59 +0.8 2,446,523 + +3.6 2,446,524 - -1.8 2,446,524 - -2.6 2,446,657 - -3.0 2,446,657 - -2.4 2,446,732 - PP_2145 0.12 +0.6 2,446,826 + PP_2145 0.21 -1.8 2,446,827 - PP_2145 0.21 -2.6 2,447,257 + PP_2145 0.64 +0.1 2,447,258 - PP_2145 0.64 -3.4 2,447,258 - PP_2145 0.64 -1.4 2,447,258 - PP_2145 0.64 -1.8 2,447,258 - PP_2145 0.64 -1.5 2,447,434 - PP_2145 0.81 -3.4 2,447,658 - -2.1 2,447,668 - -1.4 2,447,744 + -1.7 2,447,744 + -0.3 2,447,745 - -0.6 2,447,745 - -0.6 2,447,967 + -1.1 2,448,056 + +0.6 2,448,459 + PP_2146 0.21 -0.7 2,448,459 + PP_2146 0.21 +0.2 2,448,460 - PP_2146 0.21 -0.1 2,448,460 - PP_2146 0.21 +1.9 2,448,460 - PP_2146 0.21 -0.9 2,448,460 - PP_2146 0.21 -0.5 2,448,463 + PP_2146 0.21 -0.4 2,448,463 + PP_2146 0.21 -0.1
Or see this region's nucleotide sequence