Strain Fitness in Pseudomonas putida KT2440 around PP_0732

Experiment: P. putida PUT10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0731 and PP_0732 are separated by 804 nucleotidesPP_0732 and PP_0733 overlap by 4 nucleotides PP_0731: PP_0731 - phosphatidylcholine synthase, at 848,582 to 850,417 _0731 PP_0732: PP_0732 - Glutamyl-tRNA reductase, at 851,222 to 852,499 _0732 PP_0733: PP_0733 - Peptide chain release factor 1, at 852,496 to 853,578 _0733 Position (kb) 851 852 853Strain fitness (log2 ratio) -2 -1 0 1at 850.294 kb on + strandat 850.433 kb on + strandat 850.434 kb on - strandat 850.740 kb on + strandat 850.741 kb on - strandat 850.741 kb on - strandat 850.741 kb on - strandat 850.926 kb on + strandat 851.103 kb on - strandat 851.112 kb on + strandat 851.113 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1
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850,294 + -0.7
850,433 + -0.6
850,434 - +0.1
850,740 + -0.1
850,741 - -0.8
850,741 - +0.3
850,741 - -1.0
850,926 + -0.0
851,103 - -0.7
851,112 + +0.2
851,113 - -2.3

Or see this region's nucleotide sequence