Experiment: P. putida PUT10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_t69 and PP_0723 are separated by 20 nucleotides PP_0723 and PP_0724 are separated by 4 nucleotides PP_0724 and PP_0725 are separated by 4 nucleotides
PP_t69: PP_t69 - tRNA-Gln, at 839,746 to 839,820
_t69
PP_0723: PP_0723 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 839,841 to 840,701
_0723
PP_0724: PP_0724 - Outer-membrane lipoprotein LolB, at 840,706 to 841,323
_0724
PP_0725: PP_0725 - conserved hypothetical TPR repeat protein, at 841,328 to 843,049
_0725
Position (kb) 840
841
842 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 839.722 kb on - strand at 839.722 kb on - strand at 839.724 kb on - strand at 839.724 kb on - strand at 839.736 kb on - strand at 839.738 kb on - strand at 839.877 kb on - strand at 839.877 kb on - strand at 841.376 kb on - strand at 841.413 kb on - strand at 841.513 kb on - strand, within PP_0725 at 841.631 kb on - strand, within PP_0725 at 841.633 kb on - strand, within PP_0725 at 841.660 kb on - strand, within PP_0725 at 841.660 kb on - strand, within PP_0725 at 841.774 kb on - strand, within PP_0725 at 841.774 kb on - strand, within PP_0725 at 841.774 kb on - strand, within PP_0725 at 841.881 kb on - strand, within PP_0725 at 841.894 kb on - strand, within PP_0725 at 842.098 kb on - strand, within PP_0725 at 842.098 kb on - strand, within PP_0725 at 842.278 kb on - strand, within PP_0725
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1 remove 839,722 - +0.8 839,722 - +0.6 839,724 - -0.1 839,724 - +0.2 839,736 - +0.7 839,738 - +0.8 839,877 - -0.1 839,877 - +2.1 841,376 - -1.2 841,413 - +0.2 841,513 - PP_0725 0.11 -2.9 841,631 - PP_0725 0.18 -1.2 841,633 - PP_0725 0.18 -1.9 841,660 - PP_0725 0.19 -2.9 841,660 - PP_0725 0.19 -2.4 841,774 - PP_0725 0.26 -0.2 841,774 - PP_0725 0.26 -1.7 841,774 - PP_0725 0.26 -1.5 841,881 - PP_0725 0.32 -1.7 841,894 - PP_0725 0.33 -2.6 842,098 - PP_0725 0.45 -2.7 842,098 - PP_0725 0.45 -1.0 842,278 - PP_0725 0.55 -2.4
Or see this region's nucleotide sequence