Experiment: P. putida PUT10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0243 and PP_0244 are separated by 153 nucleotides PP_0244 and PP_0245 overlap by 1 nucleotides PP_0245 and PP_0246 overlap by 44 nucleotides PP_0246 and PP_0247 are separated by 13 nucleotides
PP_0243: PP_0243 - Glutamate--cysteine ligase, at 296,426 to 298,003
_0243
PP_0244: PP_0244 - conserved protein of unknown function, at 298,157 to 298,540
_0244
PP_0245: PP_0245 - putative transcriptional accessory protein, at 298,540 to 301,125
_0245
PP_0246: PP_0246 - two-component system DNA-binding response transcriptional dual regulator, at 301,082 to 301,822
_0246
PP_0247: PP_0247 - Osmolarity sensor protein EnvZ, at 301,836 to 303,149
_0247
Position (kb) 298
299
300
301
302 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 298.008 kb on + strand at 298.009 kb on - strand at 298.049 kb on + strand at 298.057 kb on + strand at 298.057 kb on + strand at 298.066 kb on - strand at 298.066 kb on - strand at 298.066 kb on - strand at 298.143 kb on + strand at 298.144 kb on - strand at 298.158 kb on + strand at 298.158 kb on + strand at 298.158 kb on + strand at 298.159 kb on - strand at 298.159 kb on - strand at 298.211 kb on + strand, within PP_0244 at 298.211 kb on + strand at 298.211 kb on + strand, within PP_0244 at 298.211 kb on + strand, within PP_0244 at 298.212 kb on - strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.300 kb on - strand, within PP_0244 at 298.521 kb on + strand at 298.522 kb on - strand at 298.532 kb on + strand at 298.532 kb on + strand at 298.533 kb on - strand at 298.541 kb on + strand at 298.541 kb on + strand at 298.541 kb on + strand at 298.542 kb on - strand at 298.833 kb on + strand, within PP_0245 at 298.834 kb on - strand, within PP_0245 at 298.834 kb on - strand at 298.888 kb on - strand, within PP_0245 at 299.022 kb on - strand, within PP_0245 at 299.462 kb on + strand, within PP_0245 at 299.462 kb on + strand, within PP_0245 at 299.463 kb on - strand, within PP_0245 at 299.658 kb on - strand, within PP_0245 at 299.804 kb on + strand, within PP_0245 at 299.805 kb on - strand, within PP_0245 at 300.014 kb on + strand, within PP_0245 at 300.015 kb on - strand, within PP_0245 at 300.015 kb on - strand, within PP_0245 at 300.222 kb on + strand, within PP_0245 at 300.223 kb on - strand, within PP_0245 at 300.223 kb on - strand, within PP_0245 at 300.248 kb on + strand, within PP_0245 at 300.248 kb on + strand, within PP_0245 at 300.249 kb on - strand, within PP_0245 at 300.323 kb on + strand, within PP_0245 at 300.324 kb on - strand, within PP_0245 at 300.324 kb on - strand, within PP_0245 at 300.380 kb on + strand, within PP_0245 at 300.381 kb on - strand, within PP_0245 at 300.381 kb on - strand, within PP_0245 at 300.551 kb on + strand, within PP_0245 at 300.561 kb on - strand, within PP_0245 at 300.561 kb on - strand, within PP_0245 at 300.561 kb on - strand, within PP_0245 at 300.843 kb on + strand, within PP_0245 at 300.866 kb on + strand, within PP_0245 at 300.867 kb on - strand at 300.867 kb on - strand at 300.888 kb on + strand at 300.889 kb on - strand at 300.943 kb on + strand at 300.943 kb on + strand at 300.943 kb on + strand at 300.943 kb on + strand at 300.944 kb on - strand at 300.944 kb on - strand at 300.944 kb on - strand at 300.945 kb on + strand at 300.946 kb on - strand at 300.946 kb on - strand at 300.946 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.185 kb on - strand, within PP_0246 at 301.403 kb on + strand, within PP_0246 at 301.527 kb on - strand, within PP_0246 at 301.761 kb on - strand at 301.761 kb on - strand at 301.787 kb on + strand at 301.787 kb on + strand at 301.788 kb on - strand at 301.932 kb on + strand at 301.933 kb on - strand at 301.972 kb on - strand, within PP_0247 at 301.972 kb on - strand, within PP_0247 at 302.004 kb on + strand, within PP_0247 at 302.005 kb on - strand, within PP_0247 at 302.005 kb on - strand, within PP_0247 at 302.058 kb on - strand, within PP_0247 at 302.058 kb on - strand, within PP_0247 at 302.106 kb on + strand, within PP_0247 at 302.106 kb on + strand, within PP_0247 at 302.118 kb on + strand, within PP_0247 at 302.118 kb on + strand, within PP_0247 at 302.118 kb on + strand, within PP_0247 at 302.118 kb on + strand, within PP_0247 at 302.119 kb on - strand, within PP_0247 at 302.119 kb on - strand, within PP_0247 at 302.119 kb on - strand, within PP_0247
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1 remove 298,008 + +0.8 298,009 - +0.3 298,049 + -2.4 298,057 + +1.3 298,057 + +2.3 298,066 - +1.0 298,066 - -0.8 298,066 - -1.1 298,143 + -0.2 298,144 - -0.3 298,158 + +0.0 298,158 + -2.2 298,158 + -0.0 298,159 - -1.3 298,159 - +0.5 298,211 + PP_0244 0.14 -0.3 298,211 + -2.5 298,211 + PP_0244 0.14 +1.0 298,211 + PP_0244 0.14 -1.1 298,212 - PP_0244 0.14 +1.1 298,299 + PP_0244 0.37 +0.3 298,299 + PP_0244 0.37 +0.1 298,299 + PP_0244 0.37 -1.2 298,299 + PP_0244 0.37 -1.3 298,300 - PP_0244 0.37 -1.9 298,521 + -0.4 298,522 - +0.0 298,532 + -0.5 298,532 + +0.2 298,533 - +0.3 298,541 + -0.7 298,541 + -0.5 298,541 + -1.2 298,542 - +0.8 298,833 + PP_0245 0.11 -1.6 298,834 - PP_0245 0.11 -2.1 298,834 - -0.5 298,888 - PP_0245 0.13 -1.2 299,022 - PP_0245 0.19 -1.1 299,462 + PP_0245 0.36 -2.2 299,462 + PP_0245 0.36 -4.6 299,463 - PP_0245 0.36 -0.9 299,658 - PP_0245 0.43 -1.1 299,804 + PP_0245 0.49 -1.0 299,805 - PP_0245 0.49 -1.0 300,014 + PP_0245 0.57 +0.4 300,015 - PP_0245 0.57 +1.5 300,015 - PP_0245 0.57 -1.3 300,222 + PP_0245 0.65 -0.7 300,223 - PP_0245 0.65 -1.7 300,223 - PP_0245 0.65 -0.7 300,248 + PP_0245 0.66 +0.1 300,248 + PP_0245 0.66 +0.9 300,249 - PP_0245 0.66 -1.9 300,323 + PP_0245 0.69 -1.9 300,324 - PP_0245 0.69 -3.7 300,324 - PP_0245 0.69 -0.2 300,380 + PP_0245 0.71 +0.7 300,381 - PP_0245 0.71 -0.8 300,381 - PP_0245 0.71 -1.1 300,551 + PP_0245 0.78 +0.1 300,561 - PP_0245 0.78 -0.0 300,561 - PP_0245 0.78 -1.0 300,561 - PP_0245 0.78 -0.3 300,843 + PP_0245 0.89 +0.2 300,866 + PP_0245 0.90 +0.8 300,867 - -2.8 300,867 - -3.7 300,888 + +0.9 300,889 - -0.1 300,943 + +0.8 300,943 + +1.0 300,943 + -3.0 300,943 + +0.6 300,944 - -1.7 300,944 - +0.1 300,944 - -1.9 300,945 + +1.1 300,946 - -0.7 300,946 - -1.9 300,946 - -0.8 301,078 - +1.8 301,078 - +2.7 301,185 - PP_0246 0.14 +1.3 301,403 + PP_0246 0.43 +1.4 301,527 - PP_0246 0.60 +1.3 301,761 - +1.6 301,761 - +2.2 301,787 + +1.0 301,787 + +1.8 301,788 - +1.3 301,932 + +1.1 301,933 - +2.0 301,972 - PP_0247 0.10 +1.3 301,972 - PP_0247 0.10 +1.6 302,004 + PP_0247 0.13 +1.2 302,005 - PP_0247 0.13 +0.6 302,005 - PP_0247 0.13 +2.6 302,058 - PP_0247 0.17 +1.6 302,058 - PP_0247 0.17 +1.8 302,106 + PP_0247 0.21 +0.7 302,106 + PP_0247 0.21 +0.6 302,118 + PP_0247 0.21 +1.1 302,118 + PP_0247 0.21 +1.0 302,118 + PP_0247 0.21 +0.9 302,118 + PP_0247 0.21 +1.2 302,119 - PP_0247 0.22 +1.8 302,119 - PP_0247 0.22 +1.5 302,119 - PP_0247 0.22 +0.7
Or see this region's nucleotide sequence