Strain Fitness in Pseudomonas putida KT2440 around PP_0187

Experiment: P. putida PUT10 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0186 and PP_0187 overlap by 4 nucleotidesPP_0187 and PP_0188 are separated by 18 nucleotides PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923 _0186 PP_0187: PP_0187 - Uroporphyrinogen-III synthase, at 238,920 to 239,687 _0187 PP_0188: PP_0188 - putative Uroporphyrin-III C-methyltransferase, at 239,706 to 240,833 _0188 Position (kb) 238 239 240Strain fitness (log2 ratio) -2 -1 0 1 2 3at 239.685 kb on + strandat 239.685 kb on + strandat 239.685 kb on + strandat 239.685 kb on + strandat 239.685 kb on + strandat 239.686 kb on - strandat 239.693 kb on + strandat 239.694 kb on - strandat 239.694 kb on - strandat 239.730 kb on + strandat 239.730 kb on + strandat 239.731 kb on - strandat 239.731 kb on - strandat 239.731 kb on - strandat 239.762 kb on + strandat 239.763 kb on - strandat 239.763 kb on - strandat 239.763 kb on - strandat 239.872 kb on + strand, within PP_0188at 239.872 kb on + strand, within PP_0188at 239.873 kb on - strand, within PP_0188at 240.028 kb on + strand, within PP_0188at 240.161 kb on + strand, within PP_0188at 240.323 kb on + strand, within PP_0188at 240.324 kb on - strand, within PP_0188at 240.365 kb on - strand, within PP_0188at 240.402 kb on - strand, within PP_0188at 240.475 kb on + strand, within PP_0188at 240.483 kb on + strand, within PP_0188at 240.484 kb on - strand, within PP_0188at 240.575 kb on + strand, within PP_0188at 240.576 kb on - strand, within PP_0188at 240.652 kb on + strand, within PP_0188at 240.653 kb on - strand, within PP_0188

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Per-strain Table

Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1
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239,685 + -0.4
239,685 + -1.1
239,685 + -1.2
239,685 + -2.4
239,685 + -0.9
239,686 - -0.5
239,693 + +0.7
239,694 - -1.1
239,694 - -1.2
239,730 + -1.4
239,730 + +0.5
239,731 - +0.7
239,731 - +0.4
239,731 - +2.8
239,762 + +0.5
239,763 - +0.8
239,763 - +0.0
239,763 - -0.9
239,872 + PP_0188 0.15 -0.5
239,872 + PP_0188 0.15 +0.3
239,873 - PP_0188 0.15 -0.7
240,028 + PP_0188 0.29 +0.2
240,161 + PP_0188 0.40 +0.2
240,323 + PP_0188 0.55 -0.9
240,324 - PP_0188 0.55 +0.4
240,365 - PP_0188 0.58 -0.6
240,402 - PP_0188 0.62 -0.8
240,475 + PP_0188 0.68 -0.5
240,483 + PP_0188 0.69 -0.3
240,484 - PP_0188 0.69 -0.3
240,575 + PP_0188 0.77 +0.5
240,576 - PP_0188 0.77 +0.5
240,652 + PP_0188 0.84 -1.9
240,653 - PP_0188 0.84 +0.3

Or see this region's nucleotide sequence