Experiment: P. putida PUT10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0072 and PP_0073 are separated by 132 nucleotides PP_0073 and PP_0074 are separated by 25 nucleotides PP_0074 and PP_0075 are separated by 129 nucleotides
PP_0072: PP_0072 - quinone oxidoreductase, at 82,151 to 83,128
_0072
PP_0073: PP_0073 - Coproporphyrinogen-III oxidase, aerobic, at 83,261 to 84,172
_0073
PP_0074: PP_0074 - shikimate 5-dehydrogenase, at 84,198 to 85,022
_0074
PP_0075: PP_0075 - putative choline sulfate transporter, at 85,152 to 86,717
_0075
Position (kb) 83
84
85 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 82.323 kb on + strand, within PP_0072 at 82.323 kb on + strand, within PP_0072 at 82.323 kb on + strand, within PP_0072 at 82.323 kb on + strand, within PP_0072 at 82.323 kb on + strand, within PP_0072 at 82.323 kb on + strand, within PP_0072 at 82.323 kb on + strand, within PP_0072 at 82.323 kb on + strand, within PP_0072 at 82.323 kb on + strand, within PP_0072 at 82.324 kb on - strand, within PP_0072 at 82.324 kb on - strand, within PP_0072 at 82.350 kb on + strand, within PP_0072 at 82.351 kb on - strand, within PP_0072 at 82.351 kb on - strand, within PP_0072 at 82.351 kb on - strand, within PP_0072 at 82.351 kb on - strand, within PP_0072 at 82.488 kb on + strand, within PP_0072 at 82.488 kb on + strand, within PP_0072 at 82.488 kb on + strand, within PP_0072 at 82.488 kb on + strand, within PP_0072 at 82.489 kb on - strand, within PP_0072 at 82.489 kb on - strand, within PP_0072 at 82.489 kb on - strand, within PP_0072 at 82.527 kb on - strand, within PP_0072 at 82.671 kb on - strand, within PP_0072 at 82.671 kb on - strand, within PP_0072 at 82.671 kb on - strand, within PP_0072 at 82.671 kb on - strand, within PP_0072 at 82.722 kb on + strand, within PP_0072 at 82.722 kb on + strand, within PP_0072 at 82.722 kb on + strand, within PP_0072 at 82.722 kb on + strand, within PP_0072 at 82.722 kb on + strand, within PP_0072 at 82.723 kb on - strand, within PP_0072 at 82.723 kb on - strand, within PP_0072 at 82.723 kb on - strand, within PP_0072 at 82.740 kb on + strand, within PP_0072 at 82.740 kb on + strand, within PP_0072 at 82.740 kb on + strand, within PP_0072 at 82.740 kb on + strand, within PP_0072 at 82.740 kb on + strand, within PP_0072 at 82.740 kb on + strand, within PP_0072 at 82.741 kb on - strand, within PP_0072 at 82.827 kb on - strand, within PP_0072 at 82.831 kb on + strand, within PP_0072 at 82.831 kb on + strand, within PP_0072 at 82.831 kb on + strand, within PP_0072 at 82.831 kb on + strand, within PP_0072 at 82.832 kb on - strand, within PP_0072 at 82.832 kb on - strand, within PP_0072 at 82.846 kb on - strand, within PP_0072 at 82.887 kb on - strand, within PP_0072 at 82.887 kb on - strand, within PP_0072 at 82.887 kb on - strand, within PP_0072 at 82.887 kb on - strand, within PP_0072 at 82.887 kb on - strand, within PP_0072 at 82.923 kb on - strand, within PP_0072 at 82.923 kb on - strand, within PP_0072 at 82.975 kb on - strand, within PP_0072 at 82.975 kb on - strand, within PP_0072 at 82.992 kb on + strand, within PP_0072 at 82.993 kb on - strand, within PP_0072 at 82.993 kb on - strand, within PP_0072 at 82.996 kb on + strand, within PP_0072 at 83.141 kb on + strand at 83.142 kb on - strand at 83.217 kb on + strand at 83.217 kb on + strand at 83.218 kb on - strand at 83.218 kb on - strand at 84.170 kb on + strand at 84.171 kb on - strand at 84.187 kb on + strand at 84.207 kb on + strand at 85.077 kb on + strand at 85.078 kb on - strand at 85.078 kb on - strand at 85.078 kb on - strand at 85.078 kb on - strand at 85.084 kb on + strand at 85.085 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1 remove 82,323 + PP_0072 0.18 +1.0 82,323 + PP_0072 0.18 -1.0 82,323 + PP_0072 0.18 +0.5 82,323 + PP_0072 0.18 +0.4 82,323 + PP_0072 0.18 -1.1 82,323 + PP_0072 0.18 -1.2 82,323 + PP_0072 0.18 -1.8 82,323 + PP_0072 0.18 -1.0 82,323 + PP_0072 0.18 +0.4 82,324 - PP_0072 0.18 -0.7 82,324 - PP_0072 0.18 +0.2 82,350 + PP_0072 0.20 +0.1 82,351 - PP_0072 0.20 +0.6 82,351 - PP_0072 0.20 -0.6 82,351 - PP_0072 0.20 +0.8 82,351 - PP_0072 0.20 +0.3 82,488 + PP_0072 0.34 +0.0 82,488 + PP_0072 0.34 +0.1 82,488 + PP_0072 0.34 -0.4 82,488 + PP_0072 0.34 +0.8 82,489 - PP_0072 0.35 +0.4 82,489 - PP_0072 0.35 +0.2 82,489 - PP_0072 0.35 +0.3 82,527 - PP_0072 0.38 -2.3 82,671 - PP_0072 0.53 -0.2 82,671 - PP_0072 0.53 +0.1 82,671 - PP_0072 0.53 -0.4 82,671 - PP_0072 0.53 +0.4 82,722 + PP_0072 0.58 -0.2 82,722 + PP_0072 0.58 -0.8 82,722 + PP_0072 0.58 -0.3 82,722 + PP_0072 0.58 -0.2 82,722 + PP_0072 0.58 +3.5 82,723 - PP_0072 0.58 -0.6 82,723 - PP_0072 0.58 +0.8 82,723 - PP_0072 0.58 -1.8 82,740 + PP_0072 0.60 -1.0 82,740 + PP_0072 0.60 -0.3 82,740 + PP_0072 0.60 -1.6 82,740 + PP_0072 0.60 +0.3 82,740 + PP_0072 0.60 +1.3 82,740 + PP_0072 0.60 -0.1 82,741 - PP_0072 0.60 -1.5 82,827 - PP_0072 0.69 +0.1 82,831 + PP_0072 0.70 +0.1 82,831 + PP_0072 0.70 -0.6 82,831 + PP_0072 0.70 +0.3 82,831 + PP_0072 0.70 +0.5 82,832 - PP_0072 0.70 +0.4 82,832 - PP_0072 0.70 +0.4 82,846 - PP_0072 0.71 -0.2 82,887 - PP_0072 0.75 -0.3 82,887 - PP_0072 0.75 +0.1 82,887 - PP_0072 0.75 +0.2 82,887 - PP_0072 0.75 -0.5 82,887 - PP_0072 0.75 -0.3 82,923 - PP_0072 0.79 +0.9 82,923 - PP_0072 0.79 -0.5 82,975 - PP_0072 0.84 +0.4 82,975 - PP_0072 0.84 +0.9 82,992 + PP_0072 0.86 +0.2 82,993 - PP_0072 0.86 +0.3 82,993 - PP_0072 0.86 +0.1 82,996 + PP_0072 0.86 -0.8 83,141 + -0.2 83,142 - +0.5 83,217 + -0.3 83,217 + -0.9 83,218 - -0.5 83,218 - -0.1 84,170 + +0.0 84,171 - -0.2 84,187 + -0.9 84,207 + -0.9 85,077 + +0.2 85,078 - -0.1 85,078 - -0.7 85,078 - +0.5 85,078 - -0.5 85,084 + -1.2 85,085 - +0.5
Or see this region's nucleotide sequence