Experiment: P. putida PUT10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0067 and PP_0068 are separated by 60 nucleotides PP_0068 and PP_0069 are separated by 206 nucleotides PP_0069 and PP_0070 are separated by 42 nucleotides PP_0070 and PP_5425 are separated by 133 nucleotides
PP_0067: PP_0067 - Methionyl-tRNA formyltransferase, at 77,540 to 78,472
_0067
PP_0068: PP_0068 - peptide deformylase, at 78,533 to 79,039
_0068
PP_0069: PP_0069 - putative Rossmann fold nucleotide-binding protein, at 79,246 to 80,343
_0069
PP_0070: PP_0070 - Threonylcarbamoyl-AMP synthase, at 80,386 to 80,943
_0070
PP_5425: PP_5425 - conserved protein of unknown function, at 81,077 to 81,277
_5425
Position (kb)
79
80
81 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 79.040 kb on - strand at 79.040 kb on - strand at 79.086 kb on - strand at 79.086 kb on - strand at 79.086 kb on - strand at 79.128 kb on - strand at 79.128 kb on - strand at 79.137 kb on - strand at 79.137 kb on - strand at 79.148 kb on - strand at 79.182 kb on - strand at 79.213 kb on + strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.454 kb on + strand, within PP_0069 at 79.455 kb on - strand, within PP_0069 at 79.476 kb on + strand, within PP_0069 at 79.477 kb on - strand, within PP_0069 at 79.477 kb on - strand, within PP_0069 at 79.477 kb on - strand, within PP_0069 at 79.477 kb on - strand, within PP_0069 at 79.707 kb on + strand, within PP_0069 at 79.708 kb on - strand, within PP_0069 at 79.724 kb on + strand, within PP_0069 at 80.029 kb on + strand, within PP_0069 at 80.029 kb on + strand, within PP_0069 at 80.029 kb on + strand, within PP_0069 at 80.030 kb on - strand, within PP_0069 at 80.341 kb on + strand at 80.341 kb on + strand at 80.345 kb on + strand at 80.345 kb on + strand at 80.346 kb on - strand at 80.346 kb on - strand at 80.346 kb on - strand at 80.365 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.945 kb on - strand at 80.945 kb on - strand at 81.007 kb on + strand at 81.007 kb on + strand at 81.007 kb on + strand at 81.116 kb on + strand, within PP_5425 at 81.116 kb on + strand, within PP_5425 at 81.116 kb on + strand, within PP_5425 at 81.117 kb on - strand, within PP_5425 at 81.183 kb on - strand, within PP_5425 at 81.183 kb on - strand, within PP_5425 at 81.205 kb on + strand, within PP_5425 at 81.206 kb on - strand, within PP_5425 at 81.206 kb on - strand, within PP_5425 at 81.244 kb on - strand, within PP_5425 at 81.244 kb on - strand, within PP_5425 at 81.270 kb on + strand at 81.271 kb on - strand at 81.318 kb on + strand at 81.334 kb on + strand at 81.334 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 1 remove 79,040 - -1.6 79,040 - -1.4 79,086 - -1.0 79,086 - -0.1 79,086 - -2.6 79,128 - -0.5 79,128 - -0.5 79,137 - -0.7 79,137 - +0.1 79,148 - -1.9 79,182 - -0.1 79,213 + -3.3 79,214 - +1.0 79,214 - +0.8 79,214 - -0.9 79,214 - -0.5 79,298 + -1.4 79,298 + +0.9 79,298 + -0.7 79,298 + -3.1 79,454 + PP_0069 0.19 -0.8 79,455 - PP_0069 0.19 +0.2 79,476 + PP_0069 0.21 -0.7 79,477 - PP_0069 0.21 -3.1 79,477 - PP_0069 0.21 -0.9 79,477 - PP_0069 0.21 -1.2 79,477 - PP_0069 0.21 -0.8 79,707 + PP_0069 0.42 -0.4 79,708 - PP_0069 0.42 -2.3 79,724 + PP_0069 0.44 -1.6 80,029 + PP_0069 0.71 -0.4 80,029 + PP_0069 0.71 +0.0 80,029 + PP_0069 0.71 -0.1 80,030 - PP_0069 0.71 -0.9 80,341 + -0.6 80,341 + -0.1 80,345 + -0.1 80,345 + -4.5 80,346 - -1.5 80,346 - -0.8 80,346 - -1.7 80,365 + -0.2 80,944 + +0.2 80,944 + -1.6 80,944 + +0.2 80,944 + +0.3 80,944 + -0.1 80,945 - +0.0 80,945 - -0.6 81,007 + -0.2 81,007 + +0.1 81,007 + +1.6 81,116 + PP_5425 0.19 -0.3 81,116 + PP_5425 0.19 -0.9 81,116 + PP_5425 0.19 -0.6 81,117 - PP_5425 0.20 -0.7 81,183 - PP_5425 0.53 -1.0 81,183 - PP_5425 0.53 -0.9 81,205 + PP_5425 0.64 +0.2 81,206 - PP_5425 0.64 +0.0 81,206 - PP_5425 0.64 -0.3 81,244 - PP_5425 0.83 +0.2 81,244 - PP_5425 0.83 -0.5 81,270 + +1.0 81,271 - -0.5 81,318 + -0.2 81,334 + +0.4 81,334 + -0.1
Or see this region's nucleotide sequence