Experiment: carbon source D-Mannose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjgF and pyrI are separated by 72 nucleotides pyrI and pyrB are separated by 12 nucleotides pyrB and pyrL are separated by 3 nucleotides pyrL and yjgH are separated by 280 nucleotides yjgH and yjgI are separated by 130 nucleotides
b4243: yjgF - orf, hypothetical protein (VIMSS), at 4,468,550 to 4,468,936
yjgF
b4244: pyrI - aspartate carbamoyltransferase regulatory subunit (NCBI), at 4,469,009 to 4,469,470
pyrI
b4245: pyrB - aspartate carbamoyltransferase catalytic subunit (NCBI), at 4,469,483 to 4,470,418
pyrB
b4246: pyrL - pyrBI operon leader peptide (NCBI), at 4,470,422 to 4,470,556
pyrL
b4248: yjgH - predicted mRNA endoribonuclease (NCBI), at 4,470,837 to 4,471,232
yjgH
b4249: yjgI - predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 4,471,363 to 4,472,076
yjgI
Position (kb)
4469
4470
4471 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 4468.519 kb on - strand at 4468.521 kb on + strand at 4468.521 kb on + strand at 4468.598 kb on - strand, within yjgF at 4468.615 kb on + strand, within yjgF at 4468.615 kb on + strand, within yjgF at 4468.622 kb on - strand, within yjgF at 4468.648 kb on + strand, within yjgF at 4468.658 kb on - strand at 4468.673 kb on + strand, within yjgF at 4468.743 kb on - strand, within yjgF at 4468.743 kb on - strand, within yjgF at 4468.743 kb on - strand, within yjgF at 4468.744 kb on - strand, within yjgF at 4468.744 kb on - strand, within yjgF at 4468.749 kb on + strand, within yjgF at 4468.757 kb on - strand, within yjgF at 4468.757 kb on - strand, within yjgF at 4468.860 kb on + strand, within yjgF at 4468.890 kb on - strand, within yjgF at 4468.890 kb on - strand, within yjgF at 4468.898 kb on - strand at 4468.899 kb on - strand at 4469.025 kb on + strand at 4469.025 kb on + strand at 4469.059 kb on - strand, within pyrI at 4469.059 kb on - strand, within pyrI at 4469.121 kb on - strand, within pyrI at 4469.140 kb on - strand, within pyrI at 4469.155 kb on - strand, within pyrI at 4469.157 kb on + strand, within pyrI at 4469.165 kb on - strand, within pyrI at 4469.179 kb on - strand, within pyrI at 4469.179 kb on - strand, within pyrI at 4469.186 kb on + strand, within pyrI at 4469.199 kb on + strand, within pyrI at 4469.204 kb on + strand, within pyrI at 4469.208 kb on - strand, within pyrI at 4469.218 kb on + strand, within pyrI at 4469.245 kb on + strand, within pyrI at 4469.245 kb on + strand, within pyrI at 4469.348 kb on - strand, within pyrI at 4469.366 kb on - strand, within pyrI at 4469.379 kb on - strand, within pyrI at 4469.379 kb on - strand, within pyrI at 4469.414 kb on + strand, within pyrI at 4469.422 kb on - strand, within pyrI at 4469.442 kb on - strand at 4469.501 kb on + strand at 4469.560 kb on - strand at 4469.562 kb on + strand at 4469.579 kb on + strand, within pyrB at 4469.579 kb on + strand, within pyrB at 4469.648 kb on + strand, within pyrB at 4469.715 kb on - strand, within pyrB at 4469.744 kb on - strand, within pyrB at 4469.746 kb on - strand, within pyrB at 4469.751 kb on + strand, within pyrB at 4469.777 kb on + strand, within pyrB at 4469.780 kb on + strand, within pyrB at 4469.780 kb on + strand, within pyrB at 4469.787 kb on + strand, within pyrB at 4469.793 kb on - strand, within pyrB at 4469.793 kb on - strand, within pyrB at 4469.817 kb on + strand, within pyrB at 4469.937 kb on - strand, within pyrB at 4469.945 kb on + strand, within pyrB at 4469.952 kb on + strand, within pyrB at 4469.966 kb on + strand, within pyrB at 4470.043 kb on + strand, within pyrB at 4470.104 kb on + strand, within pyrB at 4470.104 kb on + strand, within pyrB at 4470.106 kb on + strand, within pyrB at 4470.200 kb on + strand, within pyrB at 4470.224 kb on + strand, within pyrB at 4470.257 kb on + strand, within pyrB at 4470.359 kb on - strand at 4470.427 kb on - strand at 4470.427 kb on - strand at 4470.466 kb on + strand, within pyrL at 4470.466 kb on + strand, within pyrL at 4470.482 kb on - strand, within pyrL at 4470.718 kb on + strand at 4470.764 kb on + strand at 4470.783 kb on - strand at 4470.783 kb on - strand at 4470.796 kb on + strand at 4470.796 kb on + strand at 4470.808 kb on + strand at 4470.830 kb on + strand at 4470.830 kb on - strand at 4470.830 kb on - strand at 4470.840 kb on + strand at 4470.842 kb on + strand at 4470.842 kb on + strand at 4470.842 kb on - strand at 4470.898 kb on - strand, within yjgH at 4470.899 kb on - strand, within yjgH at 4470.988 kb on + strand, within yjgH at 4470.996 kb on - strand, within yjgH at 4470.996 kb on - strand, within yjgH at 4470.996 kb on - strand, within yjgH at 4471.004 kb on - strand, within yjgH at 4471.009 kb on - strand, within yjgH at 4471.012 kb on - strand, within yjgH at 4471.097 kb on + strand, within yjgH at 4471.176 kb on - strand, within yjgH at 4471.354 kb on - strand at 4471.362 kb on + strand at 4471.367 kb on + strand at 4471.378 kb on - strand at 4471.388 kb on - strand at 4471.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM remove 4,468,519 - -3.8 4,468,521 + +1.7 4,468,521 + -1.0 4,468,598 - yjgF b4243 0.12 +1.3 4,468,615 + yjgF b4243 0.17 -3.1 4,468,615 + yjgF b4243 0.17 +1.7 4,468,622 - yjgF b4243 0.19 +2.3 4,468,648 + yjgF b4243 0.25 -3.0 4,468,658 - -1.5 4,468,673 + yjgF b4243 0.32 -2.8 4,468,743 - yjgF b4243 0.50 -1.8 4,468,743 - yjgF b4243 0.50 +1.1 4,468,743 - yjgF b4243 0.50 -2.8 4,468,744 - yjgF b4243 0.50 -3.2 4,468,744 - yjgF b4243 0.50 -2.6 4,468,749 + yjgF b4243 0.51 -1.2 4,468,757 - yjgF b4243 0.53 -0.4 4,468,757 - yjgF b4243 0.53 -0.9 4,468,860 + yjgF b4243 0.80 -4.0 4,468,890 - yjgF b4243 0.88 +1.4 4,468,890 - yjgF b4243 0.88 +0.9 4,468,898 - -0.0 4,468,899 - +0.0 4,469,025 + -0.2 4,469,025 + +0.4 4,469,059 - pyrI b4244 0.11 -2.7 4,469,059 - pyrI b4244 0.11 +1.9 4,469,121 - pyrI b4244 0.24 +1.1 4,469,140 - pyrI b4244 0.28 +0.8 4,469,155 - pyrI b4244 0.32 -4.5 4,469,157 + pyrI b4244 0.32 +1.1 4,469,165 - pyrI b4244 0.34 +2.0 4,469,179 - pyrI b4244 0.37 -3.3 4,469,179 - pyrI b4244 0.37 +0.7 4,469,186 + pyrI b4244 0.38 +0.0 4,469,199 + pyrI b4244 0.41 -0.6 4,469,204 + pyrI b4244 0.42 +0.5 4,469,208 - pyrI b4244 0.43 +0.9 4,469,218 + pyrI b4244 0.45 +0.5 4,469,245 + pyrI b4244 0.51 -2.2 4,469,245 + pyrI b4244 0.51 +1.5 4,469,348 - pyrI b4244 0.73 -0.3 4,469,366 - pyrI b4244 0.77 +1.2 4,469,379 - pyrI b4244 0.80 +1.1 4,469,379 - pyrI b4244 0.80 +2.1 4,469,414 + pyrI b4244 0.88 +1.7 4,469,422 - pyrI b4244 0.89 +0.1 4,469,442 - +0.7 4,469,501 + +0.0 4,469,560 - -3.1 4,469,562 + -0.7 4,469,579 + pyrB b4245 0.10 -2.0 4,469,579 + pyrB b4245 0.10 -3.7 4,469,648 + pyrB b4245 0.18 -4.8 4,469,715 - pyrB b4245 0.25 -5.9 4,469,744 - pyrB b4245 0.28 -4.7 4,469,746 - pyrB b4245 0.28 -5.3 4,469,751 + pyrB b4245 0.29 -4.9 4,469,777 + pyrB b4245 0.31 -4.2 4,469,780 + pyrB b4245 0.32 -3.9 4,469,780 + pyrB b4245 0.32 -3.1 4,469,787 + pyrB b4245 0.32 -4.5 4,469,793 - pyrB b4245 0.33 -4.5 4,469,793 - pyrB b4245 0.33 -4.0 4,469,817 + pyrB b4245 0.36 -4.0 4,469,937 - pyrB b4245 0.49 -6.4 4,469,945 + pyrB b4245 0.49 -4.2 4,469,952 + pyrB b4245 0.50 -3.3 4,469,966 + pyrB b4245 0.52 -3.9 4,470,043 + pyrB b4245 0.60 -3.3 4,470,104 + pyrB b4245 0.66 -4.0 4,470,104 + pyrB b4245 0.66 -3.3 4,470,106 + pyrB b4245 0.67 -3.5 4,470,200 + pyrB b4245 0.77 -4.3 4,470,224 + pyrB b4245 0.79 -5.6 4,470,257 + pyrB b4245 0.83 +0.5 4,470,359 - -3.9 4,470,427 - +0.8 4,470,427 - +0.5 4,470,466 + pyrL b4246 0.33 -4.0 4,470,466 + pyrL b4246 0.33 -0.2 4,470,482 - pyrL b4246 0.44 -2.2 4,470,718 + -1.8 4,470,764 + -0.1 4,470,783 - -3.6 4,470,783 - +0.6 4,470,796 + -0.6 4,470,796 + -2.9 4,470,808 + +0.1 4,470,830 + +0.9 4,470,830 - +5.7 4,470,830 - -2.1 4,470,840 + +2.1 4,470,842 + +0.2 4,470,842 + +0.4 4,470,842 - +1.8 4,470,898 - yjgH b4248 0.15 +1.8 4,470,899 - yjgH b4248 0.16 +1.9 4,470,988 + yjgH b4248 0.38 -1.4 4,470,996 - yjgH b4248 0.40 +2.6 4,470,996 - yjgH b4248 0.40 -0.1 4,470,996 - yjgH b4248 0.40 -3.4 4,471,004 - yjgH b4248 0.42 +1.6 4,471,009 - yjgH b4248 0.43 -4.8 4,471,012 - yjgH b4248 0.44 +0.0 4,471,097 + yjgH b4248 0.66 +0.1 4,471,176 - yjgH b4248 0.86 -0.4 4,471,354 - -3.9 4,471,362 + +0.7 4,471,367 + -3.7 4,471,378 - +0.5 4,471,388 - +2.3 4,471,388 - -2.1
Or see this region's nucleotide sequence