Strain Fitness in Escherichia coli BW25113 around b3368
Experiment: carbon source D-Mannose 0.5mM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | carbon source D-Mannose 0.5mM |
---|---|---|---|---|---|
remove | |||||
3,494,888 | - | +0.1 | |||
3,494,888 | - | +1.3 | |||
3,494,939 | + | +1.4 | |||
3,494,939 | + | +0.3 | |||
3,494,947 | - | +0.0 | |||
3,494,947 | - | -0.1 | |||
3,495,048 | + | +0.8 | |||
3,495,056 | - | -0.3 | |||
3,495,062 | - | -2.5 | |||
3,495,071 | + | +0.6 | |||
3,495,079 | - | +1.1 | |||
3,495,113 | + | nirC | b3367 | 0.11 | -0.2 |
3,495,113 | + | nirC | b3367 | 0.11 | -1.2 |
3,495,123 | + | nirC | b3367 | 0.12 | -1.6 |
3,495,124 | - | nirC | b3367 | 0.12 | -1.2 |
3,495,124 | - | nirC | b3367 | 0.12 | +1.6 |
3,495,129 | + | nirC | b3367 | 0.13 | -3.1 |
3,495,129 | + | nirC | b3367 | 0.13 | +2.3 |
3,495,131 | + | nirC | b3367 | 0.13 | -0.1 |
3,495,148 | - | nirC | b3367 | 0.15 | -3.6 |
3,495,148 | - | nirC | b3367 | 0.15 | -0.9 |
3,495,148 | - | nirC | b3367 | 0.15 | +0.5 |
3,495,148 | - | nirC | b3367 | 0.15 | -2.4 |
3,495,182 | + | nirC | b3367 | 0.19 | +0.0 |
3,495,195 | - | nirC | b3367 | 0.21 | -0.2 |
3,495,197 | + | nirC | b3367 | 0.21 | -1.1 |
3,495,197 | + | nirC | b3367 | 0.21 | +1.6 |
3,495,251 | + | nirC | b3367 | 0.28 | -4.2 |
3,495,251 | + | nirC | b3367 | 0.28 | -3.8 |
3,495,251 | + | nirC | b3367 | 0.28 | +1.0 |
3,495,259 | - | nirC | b3367 | 0.29 | +1.5 |
3,495,271 | - | nirC | b3367 | 0.30 | -4.0 |
3,495,308 | + | nirC | b3367 | 0.35 | -2.9 |
3,495,308 | + | nirC | b3367 | 0.35 | +1.5 |
3,495,311 | - | nirC | b3367 | 0.35 | -0.2 |
3,495,311 | - | nirC | b3367 | 0.35 | +0.5 |
3,495,353 | - | nirC | b3367 | 0.41 | +0.8 |
3,495,358 | + | nirC | b3367 | 0.41 | +1.1 |
3,495,388 | + | nirC | b3367 | 0.45 | -3.6 |
3,495,388 | + | nirC | b3367 | 0.45 | -4.5 |
3,495,388 | + | nirC | b3367 | 0.45 | +0.9 |
3,495,388 | + | nirC | b3367 | 0.45 | +0.7 |
3,495,388 | + | nirC | b3367 | 0.45 | -3.0 |
3,495,396 | - | nirC | b3367 | 0.46 | -1.2 |
3,495,491 | + | nirC | b3367 | 0.58 | +0.9 |
3,495,535 | + | nirC | b3367 | 0.63 | +1.3 |
3,495,579 | - | nirC | b3367 | 0.69 | -4.2 |
3,495,609 | + | nirC | b3367 | 0.72 | -0.0 |
3,495,623 | - | nirC | b3367 | 0.74 | +1.5 |
3,495,623 | - | nirC | b3367 | 0.74 | -2.8 |
3,495,626 | + | nirC | b3367 | 0.74 | -4.2 |
3,495,626 | + | nirC | b3367 | 0.74 | -0.5 |
3,495,645 | + | nirC | b3367 | 0.77 | -0.1 |
3,495,645 | + | nirC | b3367 | 0.77 | +0.8 |
3,495,653 | + | nirC | b3367 | 0.78 | +0.2 |
3,495,654 | - | nirC | b3367 | 0.78 | +0.8 |
3,495,678 | - | nirC | b3367 | 0.81 | +1.1 |
3,495,678 | - | nirC | b3367 | 0.81 | -1.9 |
3,495,702 | + | nirC | b3367 | 0.84 | -4.8 |
3,495,705 | - | nirC | b3367 | 0.84 | +1.3 |
3,495,710 | - | nirC | b3367 | 0.85 | +0.3 |
3,495,715 | - | nirC | b3367 | 0.86 | +1.1 |
3,495,717 | + | nirC | b3367 | 0.86 | -3.6 |
3,495,744 | - | nirC | b3367 | 0.89 | +1.4 |
3,495,832 | - | +0.4 | |||
3,495,893 | + | -0.9 | |||
3,495,932 | - | -1.2 | |||
3,495,936 | + | -2.7 | |||
3,495,983 | + | -4.0 | |||
3,495,993 | + | cysG | b3368 | 0.10 | -3.6 |
3,496,019 | + | cysG | b3368 | 0.12 | -4.6 |
3,496,019 | + | cysG | b3368 | 0.12 | -4.1 |
3,496,019 | + | cysG | b3368 | 0.12 | -3.8 |
3,496,026 | - | cysG | b3368 | 0.13 | -5.3 |
3,496,065 | - | cysG | b3368 | 0.16 | -3.5 |
3,496,070 | - | cysG | b3368 | 0.16 | -0.9 |
3,496,100 | + | cysG | b3368 | 0.18 | -3.5 |
3,496,100 | + | cysG | b3368 | 0.18 | +0.6 |
3,496,119 | - | cysG | b3368 | 0.20 | -2.9 |
3,496,119 | - | cysG | b3368 | 0.20 | -1.0 |
3,496,237 | - | cysG | b3368 | 0.28 | -5.4 |
3,496,300 | - | cysG | b3368 | 0.33 | -0.4 |
3,496,300 | - | cysG | b3368 | 0.33 | +2.6 |
3,496,345 | + | cysG | b3368 | 0.36 | -4.5 |
3,496,351 | + | cysG | b3368 | 0.36 | -3.9 |
3,496,371 | - | cysG | b3368 | 0.38 | -3.1 |
3,496,417 | - | cysG | b3368 | 0.41 | -4.9 |
3,496,422 | - | cysG | b3368 | 0.42 | -5.0 |
3,496,456 | - | cysG | b3368 | 0.44 | -3.0 |
3,496,502 | - | cysG | b3368 | 0.47 | -2.7 |
3,496,504 | + | cysG | b3368 | 0.48 | -4.7 |
3,496,504 | + | cysG | b3368 | 0.48 | -4.6 |
3,496,514 | + | cysG | b3368 | 0.48 | -3.7 |
3,496,514 | + | cysG | b3368 | 0.48 | -4.6 |
3,496,514 | + | cysG | b3368 | 0.48 | -3.2 |
3,496,519 | - | cysG | b3368 | 0.49 | -4.2 |
3,496,529 | + | cysG | b3368 | 0.49 | -2.1 |
3,496,529 | + | cysG | b3368 | 0.49 | -3.6 |
3,496,539 | + | cysG | b3368 | 0.50 | -3.6 |
3,496,539 | + | cysG | b3368 | 0.50 | -2.8 |
3,496,582 | - | cysG | b3368 | 0.53 | -2.1 |
3,496,582 | - | cysG | b3368 | 0.53 | -2.2 |
3,496,582 | - | cysG | b3368 | 0.53 | -3.4 |
3,496,585 | + | cysG | b3368 | 0.53 | -4.3 |
3,496,585 | + | cysG | b3368 | 0.53 | -3.9 |
3,496,585 | + | cysG | b3368 | 0.53 | -3.5 |
3,496,585 | - | cysG | b3368 | 0.53 | -0.2 |
3,496,617 | - | cysG | b3368 | 0.56 | -2.4 |
3,496,622 | - | cysG | b3368 | 0.56 | -3.2 |
3,496,634 | + | cysG | b3368 | 0.57 | -4.0 |
3,496,666 | + | cysG | b3368 | 0.59 | -3.3 |
3,496,666 | + | cysG | b3368 | 0.59 | -4.3 |
3,496,675 | + | cysG | b3368 | 0.60 | -3.3 |
3,496,675 | + | cysG | b3368 | 0.60 | -2.3 |
3,496,679 | - | cysG | b3368 | 0.60 | -2.4 |
3,496,679 | - | cysG | b3368 | 0.60 | -4.0 |
3,496,682 | - | cysG | b3368 | 0.61 | -3.2 |
3,496,697 | + | cysG | b3368 | 0.62 | -3.7 |
3,496,697 | + | cysG | b3368 | 0.62 | -2.7 |
3,496,702 | + | cysG | b3368 | 0.62 | -4.6 |
3,496,702 | + | cysG | b3368 | 0.62 | -3.8 |
3,496,708 | - | cysG | b3368 | 0.62 | -2.2 |
3,496,786 | + | cysG | b3368 | 0.68 | -4.2 |
3,496,794 | - | cysG | b3368 | 0.69 | +0.1 |
3,496,797 | + | cysG | b3368 | 0.69 | -5.9 |
3,496,800 | + | cysG | b3368 | 0.69 | -3.5 |
3,496,800 | + | cysG | b3368 | 0.69 | +1.7 |
3,496,800 | + | cysG | b3368 | 0.69 | -2.3 |
3,496,823 | + | cysG | b3368 | 0.71 | -3.6 |
3,496,823 | + | cysG | b3368 | 0.71 | -3.0 |
3,496,825 | + | cysG | b3368 | 0.71 | -3.0 |
3,496,835 | + | cysG | b3368 | 0.72 | -3.7 |
3,496,841 | - | cysG | b3368 | 0.72 | -0.8 |
3,496,846 | + | cysG | b3368 | 0.72 | -4.3 |
3,496,861 | + | cysG | b3368 | 0.74 | -3.9 |
3,496,861 | + | cysG | b3368 | 0.74 | -2.4 |
3,496,861 | + | -0.0 | |||
3,496,877 | + | cysG | b3368 | 0.75 | -2.5 |
3,496,877 | + | cysG | b3368 | 0.75 | -2.4 |
3,496,892 | + | cysG | b3368 | 0.76 | -1.3 |
3,496,892 | + | cysG | b3368 | 0.76 | -2.3 |
3,496,904 | + | cysG | b3368 | 0.77 | -2.9 |
3,496,904 | + | cysG | b3368 | 0.77 | -4.4 |
3,496,942 | + | cysG | b3368 | 0.79 | -2.1 |
3,496,942 | + | cysG | b3368 | 0.79 | -2.4 |
3,496,947 | + | cysG | b3368 | 0.80 | -2.1 |
3,496,960 | - | cysG | b3368 | 0.81 | +0.6 |
3,496,981 | + | cysG | b3368 | 0.82 | -5.5 |
3,496,981 | + | cysG | b3368 | 0.82 | -3.0 |
3,496,986 | + | cysG | b3368 | 0.83 | -3.5 |
3,496,986 | + | cysG | b3368 | 0.83 | -3.9 |
3,496,986 | + | cysG | b3368 | 0.83 | -0.3 |
3,496,986 | + | cysG | b3368 | 0.83 | -5.0 |
3,496,988 | + | cysG | b3368 | 0.83 | -4.0 |
3,496,989 | + | cysG | b3368 | 0.83 | -3.2 |
3,496,989 | + | cysG | b3368 | 0.83 | -3.7 |
3,496,989 | + | cysG | b3368 | 0.83 | -1.1 |
3,496,991 | + | cysG | b3368 | 0.83 | -3.3 |
3,496,994 | - | cysG | b3368 | 0.83 | +0.8 |
3,496,994 | - | cysG | b3368 | 0.83 | -0.7 |
3,496,994 | - | cysG | b3368 | 0.83 | +1.6 |
3,496,996 | + | cysG | b3368 | 0.83 | +0.8 |
3,496,996 | + | cysG | b3368 | 0.83 | -3.6 |
3,497,012 | + | cysG | b3368 | 0.85 | -2.8 |
3,497,017 | + | cysG | b3368 | 0.85 | -2.2 |
3,497,054 | + | cysG | b3368 | 0.88 | -3.8 |
3,497,089 | + | -3.3 | |||
3,497,097 | + | -3.4 | |||
3,497,097 | + | -4.1 | |||
3,497,113 | + | -1.9 | |||
3,497,114 | + | -4.3 | |||
3,497,114 | + | -5.1 | |||
3,497,114 | + | -4.0 | |||
3,497,114 | + | -3.1 | |||
3,497,114 | + | -2.6 | |||
3,497,114 | + | -3.4 | |||
3,497,116 | + | -3.5 | |||
3,497,117 | + | -4.7 | |||
3,497,118 | + | -4.6 | |||
3,497,124 | + | -3.3 | |||
3,497,124 | + | -3.6 | |||
3,497,124 | + | -3.6 | |||
3,497,130 | - | +3.1 | |||
3,497,136 | + | -2.0 | |||
3,497,142 | + | -2.5 | |||
3,497,142 | + | -3.9 | |||
3,497,143 | + | -3.1 | |||
3,497,143 | + | -2.7 | |||
3,497,146 | + | -3.9 | |||
3,497,159 | + | -4.0 | |||
3,497,165 | + | -2.8 | |||
3,497,172 | + | -2.3 | |||
3,497,173 | + | -0.8 | |||
3,497,173 | + | -3.5 | |||
3,497,180 | + | -4.4 | |||
3,497,180 | + | -4.4 | |||
3,497,180 | + | -4.3 | |||
3,497,180 | + | -3.4 | |||
3,497,190 | + | -1.0 | |||
3,497,192 | + | -1.8 | |||
3,497,192 | + | -5.0 | |||
3,497,192 | + | -4.0 | |||
3,497,192 | + | -1.5 | |||
3,497,192 | + | +1.0 | |||
3,497,192 | + | -4.9 | |||
3,497,192 | + | -2.6 | |||
3,497,193 | + | +0.8 | |||
3,497,205 | + | -4.3 | |||
3,497,205 | + | -3.1 | |||
3,497,205 | + | -3.9 | |||
3,497,213 | + | +0.6 | |||
3,497,454 | - | -3.2 | |||
3,497,454 | - | -0.2 | |||
3,497,494 | + | yhfL | b3369 | 0.14 | +2.1 |
3,497,497 | + | yhfL | b3369 | 0.16 | +0.6 |
3,497,501 | + | yhfL | b3369 | 0.18 | -0.4 |
3,497,501 | + | yhfL | b3369 | 0.18 | -0.2 |
3,497,504 | - | yhfL | b3369 | 0.20 | -2.6 |
3,497,504 | - | yhfL | b3369 | 0.20 | +1.0 |
3,497,506 | + | yhfL | b3369 | 0.21 | -0.7 |
3,497,506 | - | yhfL | b3369 | 0.21 | -2.2 |
3,497,508 | + | yhfL | b3369 | 0.23 | +0.1 |
3,497,508 | + | yhfL | b3369 | 0.23 | +0.3 |
3,497,509 | - | yhfL | b3369 | 0.23 | -0.3 |
3,497,509 | - | yhfL | b3369 | 0.23 | +0.4 |
3,497,509 | - | yhfL | b3369 | 0.23 | +0.6 |
3,497,511 | + | yhfL | b3369 | 0.24 | +1.2 |
3,497,514 | - | yhfL | b3369 | 0.26 | -4.3 |
3,497,514 | - | yhfL | b3369 | 0.26 | -3.1 |
3,497,514 | - | yhfL | b3369 | 0.26 | +0.9 |
3,497,514 | - | yhfL | b3369 | 0.26 | +0.6 |
3,497,514 | - | yhfL | b3369 | 0.26 | +0.5 |
3,497,514 | - | yhfL | b3369 | 0.26 | +0.1 |
3,497,514 | - | yhfL | b3369 | 0.26 | -3.0 |
3,497,514 | - | yhfL | b3369 | 0.26 | -0.6 |
3,497,514 | - | yhfL | b3369 | 0.26 | +0.5 |
3,497,516 | + | yhfL | b3369 | 0.27 | -0.3 |
3,497,516 | + | yhfL | b3369 | 0.27 | -0.9 |
3,497,518 | - | yhfL | b3369 | 0.29 | -1.6 |
3,497,518 | - | yhfL | b3369 | 0.29 | +1.8 |
3,497,518 | - | yhfL | b3369 | 0.29 | -0.6 |
3,497,518 | - | yhfL | b3369 | 0.29 | +0.2 |
3,497,519 | - | yhfL | b3369 | 0.29 | +2.7 |
3,497,519 | - | yhfL | b3369 | 0.29 | +0.2 |
3,497,519 | - | yhfL | b3369 | 0.29 | -2.5 |
3,497,522 | + | yhfL | b3369 | 0.31 | -1.2 |
3,497,522 | + | yhfL | b3369 | 0.31 | -1.3 |
3,497,522 | + | yhfL | b3369 | 0.31 | +2.2 |
3,497,524 | - | yhfL | b3369 | 0.32 | +0.9 |
3,497,524 | - | yhfL | b3369 | 0.32 | -0.9 |
3,497,524 | - | yhfL | b3369 | 0.32 | +0.8 |
3,497,524 | - | yhfL | b3369 | 0.32 | +2.0 |
3,497,524 | - | yhfL | b3369 | 0.32 | -1.0 |
3,497,527 | - | yhfL | b3369 | 0.34 | -0.4 |
3,497,527 | - | yhfL | b3369 | 0.34 | +2.3 |
3,497,531 | - | yhfL | b3369 | 0.36 | +2.7 |
3,497,531 | - | yhfL | b3369 | 0.36 | -1.9 |
3,497,534 | - | yhfL | b3369 | 0.38 | +0.2 |
3,497,534 | - | yhfL | b3369 | 0.38 | +1.4 |
3,497,536 | - | yhfL | b3369 | 0.39 | -0.4 |
3,497,538 | - | yhfL | b3369 | 0.40 | -2.5 |
3,497,547 | - | yhfL | b3369 | 0.46 | -3.2 |
3,497,556 | + | yhfL | b3369 | 0.51 | +2.2 |
3,497,559 | - | yhfL | b3369 | 0.53 | -4.1 |
3,497,562 | + | yhfL | b3369 | 0.55 | +3.3 |
3,497,563 | + | yhfL | b3369 | 0.55 | -2.1 |
3,497,563 | + | yhfL | b3369 | 0.55 | +0.7 |
3,497,563 | + | yhfL | b3369 | 0.55 | -2.1 |
3,497,567 | + | yhfL | b3369 | 0.58 | -3.5 |
3,497,569 | - | yhfL | b3369 | 0.59 | +1.2 |
3,497,571 | - | yhfL | b3369 | 0.60 | -1.3 |
3,497,571 | - | yhfL | b3369 | 0.60 | +2.0 |
3,497,571 | - | yhfL | b3369 | 0.60 | -2.2 |
3,497,572 | - | yhfL | b3369 | 0.61 | -1.7 |
3,497,575 | + | yhfL | b3369 | 0.62 | -3.2 |
3,497,575 | + | yhfL | b3369 | 0.62 | -3.2 |
3,497,580 | - | yhfL | b3369 | 0.65 | +1.9 |
3,497,580 | - | yhfL | b3369 | 0.65 | -0.9 |
3,497,580 | - | yhfL | b3369 | 0.65 | +0.3 |
3,497,583 | - | yhfL | b3369 | 0.67 | +1.7 |
3,497,619 | + | yhfL | b3369 | 0.89 | -2.9 |
3,497,621 | + | -2.2 | |||
3,497,624 | + | -0.1 | |||
3,497,627 | + | +2.7 | |||
3,497,627 | - | +0.4 | |||
3,497,629 | + | +1.3 | |||
3,497,629 | + | +1.0 | |||
3,497,629 | - | -2.7 | |||
3,497,640 | - | +1.1 | |||
3,497,711 | - | -3.0 | |||
3,497,777 | - | +0.5 | |||
3,497,784 | - | +0.3 | |||
3,497,784 | - | -0.8 | |||
3,497,788 | + | +1.4 | |||
3,497,788 | + | +0.2 | |||
3,497,801 | - | +0.2 | |||
3,497,896 | + | -4.0 | |||
3,497,904 | - | -4.4 | |||
3,497,907 | - | -4.0 | |||
3,497,937 | - | -2.9 | |||
3,497,939 | + | -2.1 | |||
3,497,939 | + | -1.9 | |||
3,498,022 | - | +0.7 | |||
3,498,025 | + | -3.1 | |||
3,498,025 | + | +1.2 | |||
3,498,034 | - | -3.4 | |||
3,498,034 | - | +2.0 | |||
3,498,048 | + | -0.2 | |||
3,498,059 | + | -2.5 | |||
3,498,059 | + | -0.3 | |||
3,498,059 | + | -2.4 | |||
3,498,061 | - | +1.6 | |||
3,498,067 | - | yhfM | b3370 | 0.10 | +0.2 |
3,498,067 | - | yhfM | b3370 | 0.10 | -1.1 |
3,498,077 | - | yhfM | b3370 | 0.11 | -2.7 |
3,498,077 | - | yhfM | b3370 | 0.11 | +2.4 |
3,498,085 | + | yhfM | b3370 | 0.11 | -1.4 |
3,498,089 | + | yhfM | b3370 | 0.12 | -3.1 |
3,498,111 | - | yhfM | b3370 | 0.13 | +2.3 |
3,498,119 | + | yhfM | b3370 | 0.14 | +0.4 |
3,498,129 | + | yhfM | b3370 | 0.15 | -2.3 |
3,498,129 | + | yhfM | b3370 | 0.15 | -1.6 |
3,498,137 | - | yhfM | b3370 | 0.15 | +1.4 |
3,498,137 | - | yhfM | b3370 | 0.15 | +1.5 |
3,498,157 | + | yhfM | b3370 | 0.17 | -1.5 |
3,498,196 | + | yhfM | b3370 | 0.20 | -2.8 |
3,498,196 | + | yhfM | b3370 | 0.20 | +1.8 |
3,498,203 | + | yhfM | b3370 | 0.20 | +1.1 |
3,498,214 | + | yhfM | b3370 | 0.21 | -0.4 |
Or see this region's nucleotide sequence