Strain Fitness in Escherichia coli BW25113 around b2046

Experiment: carbon source D-Mannose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaK and wzxC are separated by 275 nucleotideswzxC and wcaJ are separated by 1 nucleotides b2045: wcaK - predicted pyruvyl transferase (NCBI), at 2,115,148 to 2,116,428 wcaK b2046: wzxC - colanic acid exporter (NCBI), at 2,116,704 to 2,118,182 wzxC b2047: wcaJ - predicted UDP-glucose lipid carrier transferase (NCBI), at 2,118,184 to 2,119,578 wcaJ Position (kb) 2116 2117 2118 2119Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2115.708 kb on + strand, within wcaKat 2115.716 kb on - strand, within wcaKat 2115.754 kb on - strand, within wcaKat 2115.790 kb on + strand, within wcaKat 2115.941 kb on + strand, within wcaKat 2115.941 kb on + strand, within wcaKat 2115.949 kb on - strand, within wcaKat 2115.959 kb on - strand, within wcaKat 2115.959 kb on - strand, within wcaKat 2115.968 kb on + strand, within wcaKat 2115.968 kb on + strand, within wcaKat 2115.976 kb on - strand, within wcaKat 2115.995 kb on + strand, within wcaKat 2116.108 kb on + strand, within wcaKat 2116.108 kb on + strand, within wcaKat 2116.413 kb on + strandat 2116.513 kb on - strandat 2116.513 kb on - strandat 2116.566 kb on - strandat 2116.566 kb on - strandat 2116.604 kb on - strandat 2116.868 kb on + strand, within wzxCat 2116.868 kb on + strand, within wzxCat 2116.880 kb on + strand, within wzxCat 2116.888 kb on - strand, within wzxCat 2116.888 kb on - strand, within wzxCat 2116.925 kb on + strand, within wzxCat 2117.172 kb on - strand, within wzxCat 2117.175 kb on + strand, within wzxCat 2117.196 kb on - strand, within wzxCat 2117.246 kb on + strand, within wzxCat 2117.262 kb on - strand, within wzxCat 2117.262 kb on - strand, within wzxCat 2117.272 kb on + strand, within wzxCat 2117.301 kb on + strand, within wzxCat 2117.431 kb on + strand, within wzxCat 2117.652 kb on - strand, within wzxCat 2117.652 kb on - strand, within wzxCat 2117.721 kb on + strand, within wzxCat 2117.877 kb on + strand, within wzxCat 2117.877 kb on + strand, within wzxCat 2118.014 kb on - strand, within wzxCat 2118.054 kb on - strandat 2118.054 kb on - strandat 2118.126 kb on - strandat 2118.353 kb on + strand, within wcaJat 2118.353 kb on + strand, within wcaJat 2118.391 kb on - strand, within wcaJat 2118.476 kb on - strand, within wcaJat 2119.085 kb on - strand, within wcaJat 2119.106 kb on - strand, within wcaJ

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM
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2,115,708 + wcaK b2045 0.44 +1.5
2,115,716 - wcaK b2045 0.44 +0.5
2,115,754 - wcaK b2045 0.47 +1.8
2,115,790 + wcaK b2045 0.50 +0.3
2,115,941 + wcaK b2045 0.62 -3.8
2,115,941 + wcaK b2045 0.62 +1.8
2,115,949 - wcaK b2045 0.63 -4.1
2,115,959 - wcaK b2045 0.63 -0.2
2,115,959 - wcaK b2045 0.63 -0.2
2,115,968 + wcaK b2045 0.64 +1.2
2,115,968 + wcaK b2045 0.64 -0.4
2,115,976 - wcaK b2045 0.65 +0.5
2,115,995 + wcaK b2045 0.66 -0.3
2,116,108 + wcaK b2045 0.75 -3.0
2,116,108 + wcaK b2045 0.75 -1.8
2,116,413 + -3.6
2,116,513 - +1.6
2,116,513 - +0.4
2,116,566 - -3.3
2,116,566 - -0.2
2,116,604 - +1.4
2,116,868 + wzxC b2046 0.11 +2.9
2,116,868 + wzxC b2046 0.11 +0.0
2,116,880 + wzxC b2046 0.12 +2.1
2,116,888 - wzxC b2046 0.12 +1.7
2,116,888 - wzxC b2046 0.12 -4.4
2,116,925 + wzxC b2046 0.15 +1.3
2,117,172 - wzxC b2046 0.32 +0.8
2,117,175 + wzxC b2046 0.32 -3.4
2,117,196 - wzxC b2046 0.33 +0.7
2,117,246 + wzxC b2046 0.37 +0.7
2,117,262 - wzxC b2046 0.38 +2.2
2,117,262 - wzxC b2046 0.38 +1.9
2,117,272 + wzxC b2046 0.38 +1.5
2,117,301 + wzxC b2046 0.40 +1.6
2,117,431 + wzxC b2046 0.49 -3.3
2,117,652 - wzxC b2046 0.64 +1.3
2,117,652 - wzxC b2046 0.64 +1.3
2,117,721 + wzxC b2046 0.69 +1.4
2,117,877 + wzxC b2046 0.79 -2.1
2,117,877 + wzxC b2046 0.79 +0.4
2,118,014 - wzxC b2046 0.89 -0.2
2,118,054 - -2.9
2,118,054 - -2.4
2,118,126 - -2.5
2,118,353 + wcaJ b2047 0.12 -1.3
2,118,353 + wcaJ b2047 0.12 +0.6
2,118,391 - wcaJ b2047 0.15 +1.0
2,118,476 - wcaJ b2047 0.21 +0.0
2,119,085 - wcaJ b2047 0.65 +1.1
2,119,106 - wcaJ b2047 0.66 +1.8

Or see this region's nucleotide sequence