Strain Fitness in Escherichia coli BW25113 around b0700

Experiment: carbon source D-Mannose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkdpA and kdpF overlap by 1 nucleotideskdpF and ybfA are separated by 312 nucleotidesybfA and rhsC are separated by 242 nucleotidesrhsC and ybfB overlap by 1 nucleotidesybfB and ybfO are separated by 30 nucleotides b0698: kdpA - potassium-transporting ATPase subunit A (NCBI), at 726,282 to 727,955 kdpA b4513: kdpF - potassium ion accessory transporter subunit (NCBI), at 727,955 to 728,044 kdpF b0699: ybfA - hypothetical protein (NCBI), at 728,357 to 728,563 ybfA b0700: rhsC - rhsC element core protein RshC (NCBI), at 728,806 to 732,999 rhsC b0702: ybfB - predicted inner membrane protein (NCBI), at 732,999 to 733,325 ybfB b0703: ybfO - conserved protein, rhs-like (RefSeq), at 733,356 to 734,876 ybfO Position (kb) 728 729 730 731 732 733Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 727.871 kb on + strandat 727.871 kb on + strandat 727.884 kb on + strandat 727.904 kb on - strandat 727.904 kb on - strandat 727.963 kb on + strandat 727.966 kb on - strandat 727.988 kb on - strand, within kdpFat 728.022 kb on - strand, within kdpFat 728.054 kb on - strandat 728.054 kb on - strandat 728.059 kb on - strandat 728.068 kb on - strandat 728.076 kb on - strandat 728.580 kb on + strandat 728.597 kb on + strandat 728.641 kb on + strandat 728.641 kb on + strandat 728.758 kb on - strandat 728.810 kb on - strandat 728.840 kb on - strandat 728.863 kb on - strandat 729.778 kb on + strand, within rhsCat 729.786 kb on - strand, within rhsCat 730.011 kb on + strand, within rhsCat 730.178 kb on - strand, within rhsCat 730.178 kb on - strand, within rhsCat 730.222 kb on + strand, within rhsCat 730.222 kb on + strand, within rhsCat 730.659 kb on - strand, within rhsCat 730.998 kb on + strand, within rhsCat 730.998 kb on + strand, within rhsCat 731.001 kb on + strand, within rhsCat 731.031 kb on - strand, within rhsCat 731.505 kb on + strand, within rhsCat 732.219 kb on - strand, within rhsCat 732.248 kb on - strand, within rhsCat 732.288 kb on + strand, within rhsCat 732.288 kb on + strand, within rhsCat 732.298 kb on - strand, within rhsCat 732.306 kb on - strand, within rhsCat 732.560 kb on + strand, within rhsCat 732.560 kb on + strand, within rhsCat 732.561 kb on + strand, within rhsCat 732.588 kb on - strandat 732.605 kb on + strandat 732.610 kb on + strandat 732.613 kb on + strandat 732.627 kb on + strandat 732.649 kb on + strandat 732.667 kb on + strandat 732.669 kb on + strandat 732.671 kb on + strandat 732.672 kb on + strandat 732.672 kb on + strandat 732.672 kb on + strandat 732.672 kb on + strandat 732.672 kb on + strandat 732.672 kb on + strandat 732.672 kb on + strandat 732.672 kb on + strandat 732.702 kb on + strandat 732.702 kb on + strandat 732.716 kb on + strandat 732.716 kb on + strandat 732.716 kb on + strandat 732.716 kb on + strandat 732.716 kb on + strandat 732.716 kb on + strandat 732.716 kb on + strandat 732.724 kb on - strandat 732.729 kb on + strandat 732.730 kb on + strandat 732.730 kb on + strandat 732.739 kb on + strandat 732.766 kb on + strandat 732.766 kb on + strandat 732.792 kb on + strandat 732.809 kb on + strandat 732.821 kb on + strandat 732.828 kb on + strandat 732.830 kb on + strandat 732.831 kb on + strandat 732.840 kb on + strandat 733.366 kb on - strandat 733.374 kb on - strandat 733.381 kb on - strandat 733.381 kb on - strandat 733.382 kb on - strandat 733.382 kb on - strandat 733.384 kb on - strandat 733.400 kb on - strandat 733.400 kb on - strandat 733.400 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM
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727,871 + +2.7
727,871 + -1.6
727,884 + +0.0
727,904 - -1.6
727,904 - +1.5
727,963 + -0.0
727,966 - +0.3
727,988 - kdpF b4513 0.37 +1.1
728,022 - kdpF b4513 0.74 +2.2
728,054 - -2.6
728,054 - -3.0
728,059 - -0.6
728,068 - -2.4
728,076 - -0.1
728,580 + +0.0
728,597 + +0.6
728,641 + +1.6
728,641 + -2.9
728,758 - +1.0
728,810 - +0.4
728,840 - +1.3
728,863 - +1.4
729,778 + rhsC b0700 0.23 +0.8
729,786 - rhsC b0700 0.23 +0.6
730,011 + rhsC b0700 0.29 +1.0
730,178 - rhsC b0700 0.33 +0.5
730,178 - rhsC b0700 0.33 +1.6
730,222 + rhsC b0700 0.34 +0.5
730,222 + rhsC b0700 0.34 +1.8
730,659 - rhsC b0700 0.44 +0.4
730,998 + rhsC b0700 0.52 -2.9
730,998 + rhsC b0700 0.52 -0.4
731,001 + rhsC b0700 0.52 -1.8
731,031 - rhsC b0700 0.53 +2.8
731,505 + rhsC b0700 0.64 +0.6
732,219 - rhsC b0700 0.81 +0.1
732,248 - rhsC b0700 0.82 +0.7
732,288 + rhsC b0700 0.83 +0.6
732,288 + rhsC b0700 0.83 -2.1
732,298 - rhsC b0700 0.83 +2.8
732,306 - rhsC b0700 0.83 +1.4
732,560 + rhsC b0700 0.90 -2.0
732,560 + rhsC b0700 0.90 +0.6
732,561 + rhsC b0700 0.90 -2.8
732,588 - +2.1
732,605 + -0.9
732,610 + +1.9
732,613 + +2.4
732,627 + +3.1
732,649 + +2.8
732,667 + +2.0
732,669 + +1.0
732,671 + +1.2
732,672 + +2.2
732,672 + -1.7
732,672 + +0.7
732,672 + +1.9
732,672 + -2.4
732,672 + -1.4
732,672 + -5.3
732,672 + -2.5
732,702 + -2.0
732,702 + +1.7
732,716 + -0.5
732,716 + -2.4
732,716 + -0.2
732,716 + -2.8
732,716 + -2.5
732,716 + +1.5
732,716 + -1.5
732,724 - +2.8
732,729 + -2.8
732,730 + -1.5
732,730 + +2.2
732,739 + +0.2
732,766 + -1.7
732,766 + -2.6
732,792 + +1.1
732,809 + +2.8
732,821 + +1.6
732,828 + -1.3
732,830 + -2.1
732,831 + +1.3
732,840 + +2.9
733,366 - +2.8
733,374 - +0.8
733,381 - +1.3
733,381 - -1.0
733,382 - -2.5
733,382 - +0.5
733,384 - -2.5
733,400 - -1.4
733,400 - -0.7
733,400 - +0.0

Or see this region's nucleotide sequence