Experiment: carbon source D-Mannose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpR and mhpA are separated by 190 nucleotides mhpA and mhpB are separated by 1 nucleotides mhpB and mhpC are separated by 2 nucleotides
b0346: mhpR - DNA-binding transcriptional activator, 3HPP-binding (RefSeq), at 366,811 to 367,644
mhpR
b0347: mhpA - 3-(3-hydroxyphenyl)propionate hydroxylase (NCBI), at 367,835 to 369,499
mhpA
b0348: mhpB - 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (NCBI), at 369,501 to 370,445
mhpB
b0349: mhpC - 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (VIMSS), at 370,448 to 371,329
mhpC
Position (kb) 367
368
369
370 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 366.960 kb on + strand, within mhpR at 366.960 kb on + strand, within mhpR at 366.960 kb on + strand, within mhpR at 366.996 kb on + strand, within mhpR at 367.173 kb on - strand, within mhpR at 367.287 kb on + strand, within mhpR at 367.325 kb on + strand, within mhpR at 367.326 kb on - strand, within mhpR at 367.592 kb on - strand at 367.592 kb on - strand at 367.596 kb on + strand at 367.728 kb on + strand at 367.728 kb on + strand at 367.736 kb on - strand at 367.736 kb on - strand at 367.736 kb on - strand at 367.738 kb on - strand at 367.784 kb on + strand at 367.784 kb on + strand at 367.784 kb on + strand at 367.792 kb on - strand at 367.792 kb on - strand at 367.792 kb on - strand at 367.865 kb on + strand at 367.865 kb on + strand at 367.926 kb on - strand at 367.940 kb on - strand at 367.942 kb on + strand at 367.942 kb on + strand at 367.942 kb on + strand at 368.079 kb on - strand, within mhpA at 368.142 kb on - strand, within mhpA at 368.261 kb on - strand, within mhpA at 368.479 kb on + strand, within mhpA at 368.486 kb on - strand, within mhpA at 368.568 kb on - strand, within mhpA at 368.661 kb on - strand, within mhpA at 368.728 kb on + strand, within mhpA at 368.728 kb on + strand, within mhpA at 368.755 kb on + strand, within mhpA at 368.761 kb on - strand, within mhpA at 368.763 kb on + strand, within mhpA at 368.763 kb on + strand, within mhpA at 368.778 kb on + strand, within mhpA at 368.883 kb on - strand, within mhpA at 368.898 kb on - strand, within mhpA at 368.898 kb on - strand, within mhpA at 369.036 kb on - strand, within mhpA at 369.077 kb on - strand, within mhpA at 369.133 kb on + strand, within mhpA at 369.232 kb on + strand, within mhpA at 369.283 kb on + strand, within mhpA at 369.418 kb on + strand at 369.625 kb on + strand, within mhpB at 369.705 kb on - strand, within mhpB at 369.705 kb on - strand, within mhpB at 369.829 kb on + strand, within mhpB at 369.829 kb on + strand, within mhpB at 369.837 kb on - strand, within mhpB at 369.908 kb on + strand, within mhpB at 369.908 kb on + strand, within mhpB at 370.056 kb on - strand, within mhpB at 370.198 kb on - strand, within mhpB at 370.263 kb on + strand, within mhpB at 370.344 kb on + strand, within mhpB at 370.364 kb on + strand at 370.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 0.5mM remove 366,960 + mhpR b0346 0.18 -1.9 366,960 + mhpR b0346 0.18 -2.5 366,960 + mhpR b0346 0.18 -1.0 366,996 + mhpR b0346 0.22 +0.3 367,173 - mhpR b0346 0.43 -1.1 367,287 + mhpR b0346 0.57 +1.6 367,325 + mhpR b0346 0.62 +1.2 367,326 - mhpR b0346 0.62 +1.6 367,592 - +0.4 367,592 - -0.9 367,596 + +0.7 367,728 + +4.1 367,728 + -3.5 367,736 - -1.6 367,736 - -3.7 367,736 - -2.6 367,738 - +0.3 367,784 + -1.9 367,784 + -0.2 367,784 + +0.6 367,792 - -2.4 367,792 - -2.1 367,792 - +0.4 367,865 + +0.6 367,865 + +1.7 367,926 - -4.4 367,940 - -0.1 367,942 + +1.5 367,942 + +1.0 367,942 + +1.1 368,079 - mhpA b0347 0.15 +0.5 368,142 - mhpA b0347 0.18 -2.9 368,261 - mhpA b0347 0.26 +0.4 368,479 + mhpA b0347 0.39 -0.7 368,486 - mhpA b0347 0.39 +0.8 368,568 - mhpA b0347 0.44 +1.6 368,661 - mhpA b0347 0.50 -1.5 368,728 + mhpA b0347 0.54 -0.9 368,728 + mhpA b0347 0.54 -0.1 368,755 + mhpA b0347 0.55 +1.0 368,761 - mhpA b0347 0.56 -0.6 368,763 + mhpA b0347 0.56 -0.4 368,763 + mhpA b0347 0.56 -3.4 368,778 + mhpA b0347 0.57 -3.6 368,883 - mhpA b0347 0.63 -1.1 368,898 - mhpA b0347 0.64 -3.4 368,898 - mhpA b0347 0.64 +0.3 369,036 - mhpA b0347 0.72 -0.5 369,077 - mhpA b0347 0.75 +0.1 369,133 + mhpA b0347 0.78 -0.2 369,232 + mhpA b0347 0.84 +0.8 369,283 + mhpA b0347 0.87 +1.2 369,418 + -3.8 369,625 + mhpB b0348 0.13 +0.9 369,705 - mhpB b0348 0.22 -1.8 369,705 - mhpB b0348 0.22 -1.6 369,829 + mhpB b0348 0.35 -4.3 369,829 + mhpB b0348 0.35 +2.7 369,837 - mhpB b0348 0.36 +1.2 369,908 + mhpB b0348 0.43 +1.5 369,908 + mhpB b0348 0.43 +1.0 370,056 - mhpB b0348 0.59 +0.6 370,198 - mhpB b0348 0.74 -2.3 370,263 + mhpB b0348 0.81 -0.3 370,344 + mhpB b0348 0.89 +0.2 370,364 + +0.7 370,398 - -0.8
Or see this region's nucleotide sequence