Experiment: P. putida PUT10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0831 and PP_t68 are separated by 330 nucleotides PP_t68 and PP_0832 are separated by 105 nucleotides PP_0832 and PP_0833 are separated by 14 nucleotides
PP_0831: PP_0831 - hypothetical protein, at 967,989 to 968,567
_0831
PP_t68: PP_t68 - tRNA-Leu, at 968,898 to 968,982
_t68
PP_0832: PP_0832 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 969,088 to 970,137
_0832
PP_0833: PP_0833 - queuine tRNA-ribosyltransferase, at 970,152 to 971,267
_0833
Position (kb) 969
970
971 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 968.120 kb on + strand, within PP_0831 at 968.121 kb on - strand, within PP_0831 at 968.270 kb on - strand, within PP_0831 at 968.288 kb on + strand, within PP_0831 at 968.389 kb on + strand, within PP_0831 at 968.389 kb on + strand, within PP_0831 at 968.389 kb on + strand, within PP_0831 at 968.389 kb on + strand, within PP_0831 at 968.436 kb on + strand, within PP_0831 at 968.436 kb on + strand, within PP_0831 at 968.436 kb on + strand, within PP_0831 at 968.437 kb on - strand, within PP_0831 at 968.581 kb on + strand at 968.582 kb on - strand at 968.599 kb on + strand at 968.599 kb on + strand at 968.600 kb on - strand at 968.764 kb on + strand at 968.791 kb on - strand at 968.791 kb on - strand at 968.802 kb on - strand at 968.802 kb on - strand at 968.876 kb on + strand at 968.877 kb on - strand at 968.877 kb on - strand at 969.055 kb on - strand at 969.232 kb on + strand, within PP_0832 at 969.232 kb on + strand, within PP_0832 at 969.233 kb on - strand, within PP_0832 at 969.395 kb on + strand, within PP_0832 at 969.396 kb on - strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.531 kb on + strand, within PP_0832 at 969.532 kb on - strand, within PP_0832 at 969.569 kb on - strand, within PP_0832 at 969.753 kb on + strand, within PP_0832 at 970.037 kb on - strand at 970.076 kb on - strand at 970.254 kb on + strand at 970.254 kb on + strand at 970.254 kb on + strand at 970.255 kb on - strand at 970.255 kb on - strand at 970.255 kb on - strand at 970.435 kb on + strand, within PP_0833 at 970.567 kb on + strand, within PP_0833 at 970.716 kb on + strand, within PP_0833 at 970.955 kb on + strand, within PP_0833 at 970.985 kb on + strand, within PP_0833 at 971.060 kb on + strand, within PP_0833 at 971.085 kb on + strand, within PP_0833 at 971.135 kb on + strand, within PP_0833
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 10 remove 968,120 + PP_0831 0.23 -0.4 968,121 - PP_0831 0.23 -0.4 968,270 - PP_0831 0.49 +0.2 968,288 + PP_0831 0.52 +0.1 968,389 + PP_0831 0.69 +2.2 968,389 + PP_0831 0.69 +0.2 968,389 + PP_0831 0.69 +1.0 968,389 + PP_0831 0.69 -0.3 968,436 + PP_0831 0.77 -0.6 968,436 + PP_0831 0.77 +0.7 968,436 + PP_0831 0.77 -0.2 968,437 - PP_0831 0.77 -1.1 968,581 + +1.7 968,582 - -0.1 968,599 + +1.2 968,599 + +0.9 968,600 - +1.1 968,764 + +1.0 968,791 - +1.1 968,791 - -0.4 968,802 - -1.1 968,802 - +0.0 968,876 + +0.5 968,877 - +1.0 968,877 - +0.8 969,055 - +1.3 969,232 + PP_0832 0.14 +3.0 969,232 + PP_0832 0.14 -1.0 969,233 - PP_0832 0.14 +4.0 969,395 + PP_0832 0.29 +1.0 969,396 - PP_0832 0.29 +2.3 969,529 + PP_0832 0.42 +2.0 969,529 + PP_0832 0.42 +1.6 969,529 + PP_0832 0.42 +2.4 969,531 + PP_0832 0.42 +1.1 969,532 - PP_0832 0.42 +2.8 969,569 - PP_0832 0.46 +1.0 969,753 + PP_0832 0.63 -0.0 970,037 - +2.3 970,076 - +0.2 970,254 + -0.8 970,254 + +2.7 970,254 + +2.1 970,255 - +4.9 970,255 - -0.0 970,255 - -0.5 970,435 + PP_0833 0.25 +3.3 970,567 + PP_0833 0.37 +1.8 970,716 + PP_0833 0.51 -1.5 970,955 + PP_0833 0.72 +2.1 970,985 + PP_0833 0.75 +1.9 971,060 + PP_0833 0.81 +1.8 971,085 + PP_0833 0.84 -0.5 971,135 + PP_0833 0.88 +2.3
Or see this region's nucleotide sequence