Strain Fitness in Escherichia coli BW25113 around b4544

Experiment: carbon source D-Mannose 1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntarnT and yfbW overlap by 4 nucleotidesyfbW and b2258 overlap by 1 nucleotidesb2258 and pmrD overlap by 7 nucleotidespmrD and menE are separated by 109 nucleotides b2257: arnT - 4-amino-4-deoxy-L-arabinose transferase (NCBI), at 2,368,930 to 2,370,582 arnT b4544: yfbW - hypothetical protein (NCBI), at 2,370,579 to 2,370,914 yfbW b2258: b2258 - putative transport/receptor protein (VIMSS), at 2,370,914 to 2,371,300 b2258 b2259: pmrD - polymyxin resistance protein B (VIMSS), at 2,371,294 to 2,371,560 pmrD b2260: menE - O-succinylbenzoic acid--CoA ligase (NCBI), at 2,371,670 to 2,373,025 menE Position (kb) 2370 2371Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2369.668 kb on + strand, within arnTat 2369.668 kb on + strand, within arnTat 2369.742 kb on + strand, within arnTat 2369.762 kb on - strand, within arnTat 2369.762 kb on - strand, within arnTat 2369.881 kb on - strand, within arnTat 2370.286 kb on - strand, within arnTat 2370.294 kb on - strand, within arnTat 2370.308 kb on + strand, within arnTat 2370.308 kb on + strand, within arnTat 2370.326 kb on - strand, within arnTat 2370.493 kb on + strandat 2370.551 kb on + strandat 2370.608 kb on - strandat 2370.715 kb on + strand, within yfbWat 2370.722 kb on - strand, within yfbWat 2370.723 kb on - strand, within yfbWat 2370.723 kb on - strand, within yfbWat 2370.747 kb on + strand, within yfbWat 2370.747 kb on + strand, within yfbWat 2370.909 kb on + strandat 2370.909 kb on - strandat 2370.911 kb on + strandat 2371.053 kb on + strand, within b2258at 2371.397 kb on - strand, within pmrDat 2371.562 kb on - strandat 2371.624 kb on - strandat 2371.624 kb on - strandat 2371.647 kb on - strandat 2371.755 kb on - strandat 2371.760 kb on - strandat 2371.831 kb on - strand, within menEat 2371.831 kb on - strand, within menE

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 1mM
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2,369,668 + arnT b2257 0.45 -0.6
2,369,668 + arnT b2257 0.45 +0.8
2,369,742 + arnT b2257 0.49 +0.4
2,369,762 - arnT b2257 0.50 -3.1
2,369,762 - arnT b2257 0.50 +2.0
2,369,881 - arnT b2257 0.58 +0.3
2,370,286 - arnT b2257 0.82 +0.5
2,370,294 - arnT b2257 0.83 -2.3
2,370,308 + arnT b2257 0.83 +0.6
2,370,308 + arnT b2257 0.83 -1.7
2,370,326 - arnT b2257 0.84 +0.8
2,370,493 + +1.0
2,370,551 + +1.1
2,370,608 - -1.1
2,370,715 + yfbW b4544 0.40 -0.6
2,370,722 - yfbW b4544 0.43 +0.7
2,370,723 - yfbW b4544 0.43 -3.4
2,370,723 - yfbW b4544 0.43 -1.2
2,370,747 + yfbW b4544 0.50 -2.3
2,370,747 + yfbW b4544 0.50 -0.8
2,370,909 + -0.5
2,370,909 - +0.8
2,370,911 + +0.7
2,371,053 + b2258 b2258 0.36 +1.8
2,371,397 - pmrD b2259 0.39 +0.9
2,371,562 - -0.2
2,371,624 - +1.0
2,371,624 - +2.1
2,371,647 - -0.0
2,371,755 - -2.0
2,371,760 - -1.0
2,371,831 - menE b2260 0.12 -0.7
2,371,831 - menE b2260 0.12 +0.0

Or see this region's nucleotide sequence