Experiment: carbon source D-Mannose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yoeD and yoeF are separated by 68 nucleotides yoeF and yeeX are separated by 100 nucleotides yeeX and yeeA are separated by 171 nucleotides yeeA and sbmC are separated by 197 nucleotides sbmC and dacD are separated by 118 nucleotides
b4642: yoeD - no description, at 2,076,573 to 2,076,701
yoeD
b4538: yoeF - hypothetical protein (RefSeq), at 2,076,770 to 2,076,955
yoeF
b2007: yeeX - conserved protein (RefSeq), at 2,077,056 to 2,077,385
yeeX
b2008: yeeA - conserved inner membrane protein (NCBI), at 2,077,557 to 2,078,615
yeeA
b2009: sbmC - DNA gyrase inhibitor (NCBI), at 2,078,813 to 2,079,286
sbmC
b2010: dacD - penicillin binding protein 6b (VIMSS), at 2,079,405 to 2,080,571
dacD
Position (kb)
2077
2078
2079 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2076.611 kb on + strand, within yoeD at 2076.670 kb on - strand, within yoeD at 2076.697 kb on + strand at 2076.881 kb on - strand, within yoeF at 2076.886 kb on - strand, within yoeF at 2076.972 kb on + strand at 2076.973 kb on - strand at 2076.988 kb on + strand at 2077.046 kb on - strand at 2077.046 kb on - strand at 2077.120 kb on + strand, within yeeX at 2077.167 kb on + strand, within yeeX at 2077.185 kb on - strand, within yeeX at 2077.364 kb on - strand at 2077.364 kb on - strand at 2077.393 kb on - strand at 2077.426 kb on - strand at 2077.541 kb on + strand at 2077.541 kb on + strand at 2077.615 kb on + strand at 2077.750 kb on + strand, within yeeA at 2077.753 kb on - strand, within yeeA at 2077.758 kb on - strand, within yeeA at 2077.800 kb on - strand, within yeeA at 2077.858 kb on - strand, within yeeA at 2077.895 kb on + strand, within yeeA at 2077.895 kb on + strand, within yeeA at 2078.047 kb on - strand, within yeeA at 2078.068 kb on + strand, within yeeA at 2078.115 kb on - strand, within yeeA at 2078.611 kb on - strand at 2078.684 kb on - strand at 2078.689 kb on - strand at 2078.853 kb on + strand at 2078.861 kb on - strand, within sbmC at 2078.864 kb on + strand, within sbmC at 2079.000 kb on + strand, within sbmC at 2079.077 kb on + strand, within sbmC at 2079.281 kb on + strand at 2079.472 kb on - strand at 2079.472 kb on - strand at 2079.505 kb on - strand at 2079.568 kb on - strand, within dacD at 2079.612 kb on + strand, within dacD
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 1mM remove 2,076,611 + yoeD b4642 0.29 -5.5 2,076,670 - yoeD b4642 0.75 -2.7 2,076,697 + +0.3 2,076,881 - yoeF b4538 0.60 +2.4 2,076,886 - yoeF b4538 0.62 +1.0 2,076,972 + +0.4 2,076,973 - +1.5 2,076,988 + +0.2 2,077,046 - -2.7 2,077,046 - +0.7 2,077,120 + yeeX b2007 0.19 +1.3 2,077,167 + yeeX b2007 0.34 +0.6 2,077,185 - yeeX b2007 0.39 -0.2 2,077,364 - -2.6 2,077,364 - -4.5 2,077,393 - -0.4 2,077,426 - +0.4 2,077,541 + +1.9 2,077,541 + +0.6 2,077,615 + -1.9 2,077,750 + yeeA b2008 0.18 -0.3 2,077,753 - yeeA b2008 0.19 -0.1 2,077,758 - yeeA b2008 0.19 +0.6 2,077,800 - yeeA b2008 0.23 +1.0 2,077,858 - yeeA b2008 0.28 -0.3 2,077,895 + yeeA b2008 0.32 +3.2 2,077,895 + yeeA b2008 0.32 -3.7 2,078,047 - yeeA b2008 0.46 -1.8 2,078,068 + yeeA b2008 0.48 +2.2 2,078,115 - yeeA b2008 0.53 -0.0 2,078,611 - -0.8 2,078,684 - -0.2 2,078,689 - -3.1 2,078,853 + -2.7 2,078,861 - sbmC b2009 0.10 +1.6 2,078,864 + sbmC b2009 0.11 +1.2 2,079,000 + sbmC b2009 0.39 -0.5 2,079,077 + sbmC b2009 0.56 +0.9 2,079,281 + +0.1 2,079,472 - +1.1 2,079,472 - -1.2 2,079,505 - -0.4 2,079,568 - dacD b2010 0.14 -0.0 2,079,612 + dacD b2010 0.18 -0.1
Or see this region's nucleotide sequence