Strain Fitness in Escherichia coli BW25113 around b2008

Experiment: carbon source D-Mannose 1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyoeD and yoeF are separated by 68 nucleotidesyoeF and yeeX are separated by 100 nucleotidesyeeX and yeeA are separated by 171 nucleotidesyeeA and sbmC are separated by 197 nucleotidessbmC and dacD are separated by 118 nucleotides b4642: yoeD - no description, at 2,076,573 to 2,076,701 yoeD b4538: yoeF - hypothetical protein (RefSeq), at 2,076,770 to 2,076,955 yoeF b2007: yeeX - conserved protein (RefSeq), at 2,077,056 to 2,077,385 yeeX b2008: yeeA - conserved inner membrane protein (NCBI), at 2,077,557 to 2,078,615 yeeA b2009: sbmC - DNA gyrase inhibitor (NCBI), at 2,078,813 to 2,079,286 sbmC b2010: dacD - penicillin binding protein 6b (VIMSS), at 2,079,405 to 2,080,571 dacD Position (kb) 2077 2078 2079Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 2076.611 kb on + strand, within yoeDat 2076.670 kb on - strand, within yoeDat 2076.697 kb on + strandat 2076.881 kb on - strand, within yoeFat 2076.886 kb on - strand, within yoeFat 2076.972 kb on + strandat 2076.973 kb on - strandat 2076.988 kb on + strandat 2077.046 kb on - strandat 2077.046 kb on - strandat 2077.120 kb on + strand, within yeeXat 2077.167 kb on + strand, within yeeXat 2077.185 kb on - strand, within yeeXat 2077.364 kb on - strandat 2077.364 kb on - strandat 2077.393 kb on - strandat 2077.426 kb on - strandat 2077.541 kb on + strandat 2077.541 kb on + strandat 2077.615 kb on + strandat 2077.750 kb on + strand, within yeeAat 2077.753 kb on - strand, within yeeAat 2077.758 kb on - strand, within yeeAat 2077.800 kb on - strand, within yeeAat 2077.858 kb on - strand, within yeeAat 2077.895 kb on + strand, within yeeAat 2077.895 kb on + strand, within yeeAat 2078.047 kb on - strand, within yeeAat 2078.068 kb on + strand, within yeeAat 2078.115 kb on - strand, within yeeAat 2078.611 kb on - strandat 2078.684 kb on - strandat 2078.689 kb on - strandat 2078.853 kb on + strandat 2078.861 kb on - strand, within sbmCat 2078.864 kb on + strand, within sbmCat 2079.000 kb on + strand, within sbmCat 2079.077 kb on + strand, within sbmCat 2079.281 kb on + strandat 2079.472 kb on - strandat 2079.472 kb on - strandat 2079.505 kb on - strandat 2079.568 kb on - strand, within dacDat 2079.612 kb on + strand, within dacD

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 1mM
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2,076,611 + yoeD b4642 0.29 -5.5
2,076,670 - yoeD b4642 0.75 -2.7
2,076,697 + +0.3
2,076,881 - yoeF b4538 0.60 +2.4
2,076,886 - yoeF b4538 0.62 +1.0
2,076,972 + +0.4
2,076,973 - +1.5
2,076,988 + +0.2
2,077,046 - -2.7
2,077,046 - +0.7
2,077,120 + yeeX b2007 0.19 +1.3
2,077,167 + yeeX b2007 0.34 +0.6
2,077,185 - yeeX b2007 0.39 -0.2
2,077,364 - -2.6
2,077,364 - -4.5
2,077,393 - -0.4
2,077,426 - +0.4
2,077,541 + +1.9
2,077,541 + +0.6
2,077,615 + -1.9
2,077,750 + yeeA b2008 0.18 -0.3
2,077,753 - yeeA b2008 0.19 -0.1
2,077,758 - yeeA b2008 0.19 +0.6
2,077,800 - yeeA b2008 0.23 +1.0
2,077,858 - yeeA b2008 0.28 -0.3
2,077,895 + yeeA b2008 0.32 +3.2
2,077,895 + yeeA b2008 0.32 -3.7
2,078,047 - yeeA b2008 0.46 -1.8
2,078,068 + yeeA b2008 0.48 +2.2
2,078,115 - yeeA b2008 0.53 -0.0
2,078,611 - -0.8
2,078,684 - -0.2
2,078,689 - -3.1
2,078,853 + -2.7
2,078,861 - sbmC b2009 0.10 +1.6
2,078,864 + sbmC b2009 0.11 +1.2
2,079,000 + sbmC b2009 0.39 -0.5
2,079,077 + sbmC b2009 0.56 +0.9
2,079,281 + +0.1
2,079,472 - +1.1
2,079,472 - -1.2
2,079,505 - -0.4
2,079,568 - dacD b2010 0.14 -0.0
2,079,612 + dacD b2010 0.18 -0.1

Or see this region's nucleotide sequence