Strain Fitness in Escherichia coli BW25113 around b1515

Experiment: carbon source D-Mannose 1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlsrC and lsrD overlap by 1 nucleotideslsrD and lsrB are separated by 11 nucleotides b1514: lsrC - AI2 transporter (NCBI), at 1,601,043 to 1,602,071 lsrC b1515: lsrD - AI2 transporter (NCBI), at 1,602,071 to 1,603,063 lsrD b1516: lsrB - AI2 transporter (NCBI), at 1,603,075 to 1,604,097 lsrB Position (kb) 1602 1603 1604Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1601.276 kb on - strand, within lsrCat 1601.423 kb on + strand, within lsrCat 1601.423 kb on + strand, within lsrCat 1601.973 kb on + strandat 1602.085 kb on + strandat 1602.439 kb on + strand, within lsrDat 1602.445 kb on - strand, within lsrDat 1602.445 kb on - strand, within lsrDat 1602.938 kb on + strand, within lsrDat 1602.946 kb on + strand, within lsrDat 1603.065 kb on - strandat 1603.134 kb on + strandat 1603.408 kb on - strand, within lsrBat 1603.487 kb on - strand, within lsrBat 1603.659 kb on - strand, within lsrBat 1603.832 kb on + strand, within lsrBat 1603.832 kb on + strand, within lsrBat 1603.997 kb on + strandat 1603.999 kb on + strandat 1603.999 kb on + strandat 1603.999 kb on + strandat 1604.043 kb on + strandat 1604.043 kb on + strandat 1604.044 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 1mM
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1,601,276 - lsrC b1514 0.23 +0.6
1,601,423 + lsrC b1514 0.37 +0.4
1,601,423 + lsrC b1514 0.37 +1.0
1,601,973 + +0.3
1,602,085 + -2.0
1,602,439 + lsrD b1515 0.37 -1.3
1,602,445 - lsrD b1515 0.38 -2.8
1,602,445 - lsrD b1515 0.38 +1.9
1,602,938 + lsrD b1515 0.87 +0.5
1,602,946 + lsrD b1515 0.88 +0.2
1,603,065 - +0.3
1,603,134 + -0.0
1,603,408 - lsrB b1516 0.33 +0.3
1,603,487 - lsrB b1516 0.40 +2.1
1,603,659 - lsrB b1516 0.57 +1.2
1,603,832 + lsrB b1516 0.74 -1.7
1,603,832 + lsrB b1516 0.74 +0.4
1,603,997 + +0.6
1,603,999 + +0.3
1,603,999 + -0.2
1,603,999 + +0.4
1,604,043 + +0.2
1,604,043 + +1.1
1,604,044 + -0.5

Or see this region's nucleotide sequence