Experiment: P. putida PUT10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4712 and PP_4713 are separated by 27 nucleotides PP_4713 and PP_4714 are separated by 59 nucleotides PP_4714 and PP_t49 are separated by 78 nucleotides PP_t49 and PP_t48 are separated by 100 nucleotides PP_t48 and PP_5706 are separated by 21 nucleotides
PP_4712: PP_4712 - Translation initiation factor IF-2, at 5,356,220 to 5,358,760
_4712
PP_4713: PP_4713 - transcription termination/antitermination L factor, at 5,358,788 to 5,360,269
_4713
PP_4714: PP_4714 - ribosome maturation factor, at 5,360,329 to 5,360,838
_4714
PP_t49: PP_t49 - tRNA-Met, at 5,360,917 to 5,360,993
_t49
PP_t48: PP_t48 - tRNA-Leu, at 5,361,094 to 5,361,179
_t48
PP_5706: PP_5706 - Sec translocation complex - SecG subunit, at 5,361,201 to 5,361,599
_5706
Position (kb) 5358
5359
5360
5361 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5358.786 kb on - strand at 5360.957 kb on - strand, within PP_t49 at 5360.973 kb on - strand, within PP_t49 at 5361.186 kb on - strand at 5361.186 kb on - strand at 5361.189 kb on - strand at 5361.189 kb on - strand at 5361.229 kb on - strand at 5361.229 kb on - strand at 5361.229 kb on - strand at 5361.229 kb on - strand at 5361.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT10 MOI 10 remove 5,358,786 - -5.6 5,360,957 - PP_t49 0.52 -3.4 5,360,973 - PP_t49 0.73 -2.4 5,361,186 - -0.8 5,361,186 - -1.3 5,361,189 - +0.3 5,361,189 - -1.0 5,361,229 - +0.0 5,361,229 - -0.1 5,361,229 - -1.3 5,361,229 - +1.0 5,361,240 - -0.4
Or see this region's nucleotide sequence