Experiment: carbon source D-Mannose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt alsK and alsE overlap by 17 nucleotides alsE and alsC are separated by 10 nucleotides alsC and alsA overlap by 22 nucleotides
b4084: alsK - D-allose kinase (NCBI), at 4,304,893 to 4,305,822
alsK
b4085: alsE - allulose-6-phosphate 3-epimerase (NCBI), at 4,305,806 to 4,306,501
alsE
b4086: alsC - D-allose transporter subunit (NCBI), at 4,306,512 to 4,307,492
alsC
b4087: alsA - fused D-allose transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 4,307,471 to 4,309,003
alsA
Position (kb)
4306
4307
4308 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4305.543 kb on - strand, within alsK at 4305.601 kb on - strand, within alsK at 4305.601 kb on - strand, within alsK at 4305.738 kb on - strand at 4305.782 kb on - strand at 4305.790 kb on - strand at 4305.832 kb on - strand at 4305.931 kb on - strand, within alsE at 4306.022 kb on - strand, within alsE at 4306.039 kb on - strand, within alsE at 4306.191 kb on - strand, within alsE at 4306.284 kb on + strand, within alsE at 4306.395 kb on + strand, within alsE at 4306.478 kb on - strand at 4306.519 kb on + strand at 4306.527 kb on - strand at 4306.538 kb on - strand at 4306.568 kb on - strand at 4306.619 kb on - strand, within alsC at 4306.678 kb on + strand, within alsC at 4306.694 kb on - strand, within alsC at 4306.750 kb on + strand, within alsC at 4306.773 kb on - strand, within alsC at 4306.773 kb on - strand, within alsC at 4306.779 kb on + strand, within alsC at 4306.804 kb on - strand, within alsC at 4306.877 kb on + strand, within alsC at 4306.901 kb on + strand, within alsC at 4306.923 kb on + strand, within alsC at 4307.043 kb on - strand, within alsC at 4307.094 kb on + strand, within alsC at 4307.101 kb on - strand, within alsC at 4307.101 kb on - strand, within alsC at 4307.102 kb on - strand, within alsC at 4307.183 kb on + strand, within alsC at 4307.252 kb on + strand, within alsC at 4307.265 kb on + strand, within alsC at 4307.422 kb on + strand at 4307.422 kb on + strand at 4307.423 kb on - strand at 4307.440 kb on - strand at 4307.451 kb on + strand at 4307.516 kb on - strand at 4307.558 kb on + strand at 4307.727 kb on + strand, within alsA at 4307.779 kb on + strand, within alsA at 4307.838 kb on + strand, within alsA at 4308.004 kb on + strand, within alsA at 4308.010 kb on - strand, within alsA at 4308.010 kb on - strand, within alsA at 4308.021 kb on - strand, within alsA at 4308.060 kb on + strand, within alsA at 4308.060 kb on + strand, within alsA at 4308.100 kb on + strand, within alsA at 4308.108 kb on - strand, within alsA at 4308.108 kb on - strand, within alsA at 4308.123 kb on - strand, within alsA at 4308.228 kb on - strand, within alsA at 4308.275 kb on + strand, within alsA at 4308.287 kb on - strand, within alsA at 4308.323 kb on - strand, within alsA at 4308.354 kb on + strand, within alsA at 4308.354 kb on + strand, within alsA at 4308.402 kb on + strand, within alsA at 4308.408 kb on - strand, within alsA at 4308.414 kb on + strand, within alsA at 4308.423 kb on + strand, within alsA at 4308.432 kb on - strand, within alsA at 4308.432 kb on - strand, within alsA at 4308.458 kb on + strand, within alsA at 4308.466 kb on + strand, within alsA at 4308.466 kb on + strand, within alsA at 4308.468 kb on + strand, within alsA at 4308.468 kb on + strand, within alsA at 4308.470 kb on + strand, within alsA at 4308.471 kb on - strand, within alsA at 4308.480 kb on + strand, within alsA at 4308.482 kb on + strand, within alsA at 4308.489 kb on + strand, within alsA
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 1mM remove 4,305,543 - alsK b4084 0.70 -3.2 4,305,601 - alsK b4084 0.76 -0.6 4,305,601 - alsK b4084 0.76 +0.1 4,305,738 - -0.3 4,305,782 - -0.6 4,305,790 - -1.8 4,305,832 - -0.4 4,305,931 - alsE b4085 0.18 +1.0 4,306,022 - alsE b4085 0.31 +0.7 4,306,039 - alsE b4085 0.33 +1.0 4,306,191 - alsE b4085 0.55 -0.8 4,306,284 + alsE b4085 0.69 +0.1 4,306,395 + alsE b4085 0.85 -0.6 4,306,478 - +0.0 4,306,519 + -3.4 4,306,527 - -0.7 4,306,538 - +0.9 4,306,568 - +0.7 4,306,619 - alsC b4086 0.11 -1.8 4,306,678 + alsC b4086 0.17 +1.2 4,306,694 - alsC b4086 0.19 -0.5 4,306,750 + alsC b4086 0.24 +0.1 4,306,773 - alsC b4086 0.27 +0.9 4,306,773 - alsC b4086 0.27 -3.0 4,306,779 + alsC b4086 0.27 -0.4 4,306,804 - alsC b4086 0.30 -1.2 4,306,877 + alsC b4086 0.37 -1.2 4,306,901 + alsC b4086 0.40 +0.3 4,306,923 + alsC b4086 0.42 +0.6 4,307,043 - alsC b4086 0.54 -4.0 4,307,094 + alsC b4086 0.59 -1.2 4,307,101 - alsC b4086 0.60 -2.0 4,307,101 - alsC b4086 0.60 -3.4 4,307,102 - alsC b4086 0.60 -1.0 4,307,183 + alsC b4086 0.68 -4.6 4,307,252 + alsC b4086 0.75 +1.2 4,307,265 + alsC b4086 0.77 -0.4 4,307,422 + +0.7 4,307,422 + -1.5 4,307,423 - +0.7 4,307,440 - +0.3 4,307,451 + -1.1 4,307,516 - -0.9 4,307,558 + +1.2 4,307,727 + alsA b4087 0.17 +1.3 4,307,779 + alsA b4087 0.20 +0.1 4,307,838 + alsA b4087 0.24 -0.5 4,308,004 + alsA b4087 0.35 -4.0 4,308,010 - alsA b4087 0.35 +0.0 4,308,010 - alsA b4087 0.35 -0.6 4,308,021 - alsA b4087 0.36 +1.1 4,308,060 + alsA b4087 0.38 -2.3 4,308,060 + alsA b4087 0.38 -0.2 4,308,100 + alsA b4087 0.41 -0.2 4,308,108 - alsA b4087 0.42 -0.6 4,308,108 - alsA b4087 0.42 -0.8 4,308,123 - alsA b4087 0.43 +0.3 4,308,228 - alsA b4087 0.49 -0.5 4,308,275 + alsA b4087 0.52 +0.6 4,308,287 - alsA b4087 0.53 +0.8 4,308,323 - alsA b4087 0.56 -0.6 4,308,354 + alsA b4087 0.58 +0.4 4,308,354 + alsA b4087 0.58 +0.1 4,308,402 + alsA b4087 0.61 -2.7 4,308,408 - alsA b4087 0.61 -3.0 4,308,414 + alsA b4087 0.62 -1.3 4,308,423 + alsA b4087 0.62 +1.0 4,308,432 - alsA b4087 0.63 +0.2 4,308,432 - alsA b4087 0.63 -0.6 4,308,458 + alsA b4087 0.64 -1.6 4,308,466 + alsA b4087 0.65 +1.5 4,308,466 + alsA b4087 0.65 -3.5 4,308,468 + alsA b4087 0.65 -2.9 4,308,468 + alsA b4087 0.65 +0.0 4,308,470 + alsA b4087 0.65 +0.4 4,308,471 - alsA b4087 0.65 -0.1 4,308,480 + alsA b4087 0.66 -1.7 4,308,482 + alsA b4087 0.66 -2.5 4,308,489 + alsA b4087 0.66 +0.7
Or see this region's nucleotide sequence