Experiment: carbon source D-Mannose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjcF and actP are separated by 177 nucleotides actP and yjcH overlap by 4 nucleotides yjcH and acs are separated by 199 nucleotides
b4066: yjcF - hypothetical protein (NCBI), at 4,279,806 to 4,281,098
yjcF
b4067: actP - acetate permease (NCBI), at 4,281,276 to 4,282,925
actP
b4068: yjcH - required for glycolate transport, with actP (from data) , at 4,282,922 to 4,283,236
yjcH
b4069: acs - acetyl-coenzyme A synthetase (NCBI), at 4,283,436 to 4,285,394
acs
Position (kb)
4281
4282
4283 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4281.018 kb on + strand at 4281.018 kb on + strand at 4281.024 kb on - strand at 4281.027 kb on + strand at 4281.046 kb on + strand at 4281.138 kb on - strand at 4281.223 kb on + strand at 4281.230 kb on - strand at 4281.230 kb on - strand at 4281.232 kb on - strand at 4281.243 kb on - strand at 4281.252 kb on - strand at 4281.252 kb on - strand at 4281.252 kb on - strand at 4281.255 kb on - strand at 4281.262 kb on + strand at 4281.262 kb on + strand at 4281.267 kb on - strand at 4281.270 kb on - strand at 4281.281 kb on - strand at 4281.281 kb on - strand at 4281.282 kb on - strand at 4281.296 kb on - strand at 4281.296 kb on - strand at 4281.296 kb on - strand at 4281.296 kb on - strand at 4281.307 kb on - strand at 4281.308 kb on - strand at 4281.308 kb on - strand at 4281.318 kb on - strand at 4281.321 kb on + strand at 4281.334 kb on - strand at 4281.334 kb on - strand at 4281.341 kb on + strand at 4281.349 kb on - strand at 4281.349 kb on - strand at 4281.349 kb on - strand at 4281.349 kb on - strand at 4281.349 kb on - strand at 4281.349 kb on - strand at 4281.412 kb on + strand at 4281.420 kb on - strand at 4281.420 kb on - strand at 4281.503 kb on + strand, within actP at 4281.503 kb on + strand, within actP at 4281.506 kb on - strand, within actP at 4281.573 kb on - strand, within actP at 4281.573 kb on - strand, within actP at 4281.578 kb on - strand, within actP at 4281.756 kb on - strand, within actP at 4281.927 kb on - strand, within actP at 4281.934 kb on + strand, within actP at 4282.003 kb on - strand, within actP at 4282.033 kb on - strand, within actP at 4282.072 kb on - strand, within actP at 4282.094 kb on - strand, within actP at 4282.191 kb on - strand, within actP at 4282.191 kb on - strand, within actP at 4282.215 kb on - strand, within actP at 4282.215 kb on - strand, within actP at 4282.412 kb on - strand, within actP at 4282.531 kb on + strand, within actP at 4282.706 kb on - strand, within actP at 4282.718 kb on - strand, within actP at 4282.718 kb on - strand, within actP at 4282.738 kb on - strand, within actP at 4282.738 kb on - strand, within actP at 4282.740 kb on - strand, within actP at 4282.842 kb on + strand at 4282.864 kb on - strand at 4282.873 kb on - strand at 4282.909 kb on + strand at 4282.938 kb on - strand at 4282.990 kb on + strand, within yjcH at 4283.005 kb on + strand, within yjcH at 4283.013 kb on - strand, within yjcH at 4283.013 kb on - strand, within yjcH at 4283.013 kb on - strand, within yjcH at 4283.050 kb on + strand, within yjcH at 4283.121 kb on - strand, within yjcH at 4283.212 kb on - strand at 4283.212 kb on - strand at 4283.218 kb on + strand at 4283.224 kb on - strand at 4283.224 kb on - strand at 4283.291 kb on - strand at 4283.315 kb on - strand at 4283.315 kb on - strand at 4283.395 kb on + strand at 4283.395 kb on + strand at 4283.423 kb on + strand at 4283.423 kb on + strand at 4283.514 kb on + strand at 4283.514 kb on + strand at 4283.595 kb on - strand at 4283.678 kb on - strand, within acs at 4283.678 kb on - strand, within acs at 4283.712 kb on + strand, within acs at 4283.749 kb on - strand, within acs at 4283.808 kb on + strand, within acs at 4283.855 kb on + strand, within acs at 4283.855 kb on + strand, within acs at 4283.865 kb on + strand, within acs
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 1mM remove 4,281,018 + -0.1 4,281,018 + +2.7 4,281,024 - +2.7 4,281,027 + +2.7 4,281,046 + +2.7 4,281,138 - -4.8 4,281,223 + +1.7 4,281,230 - +1.2 4,281,230 - +0.2 4,281,232 - -2.5 4,281,243 - +1.4 4,281,252 - +1.5 4,281,252 - +1.7 4,281,252 - +2.0 4,281,255 - -1.3 4,281,262 + -0.1 4,281,262 + +2.7 4,281,267 - +0.6 4,281,270 - +0.8 4,281,281 - +0.1 4,281,281 - +0.9 4,281,282 - -0.9 4,281,296 - +0.0 4,281,296 - +0.2 4,281,296 - +0.6 4,281,296 - +0.0 4,281,307 - -1.0 4,281,308 - +0.2 4,281,308 - -0.4 4,281,318 - -3.6 4,281,321 + -0.8 4,281,334 - -0.3 4,281,334 - -2.8 4,281,341 + +0.2 4,281,349 - +0.2 4,281,349 - -0.5 4,281,349 - +0.0 4,281,349 - +0.4 4,281,349 - -2.1 4,281,349 - +0.3 4,281,412 + +0.7 4,281,420 - -2.8 4,281,420 - +0.9 4,281,503 + actP b4067 0.14 -1.2 4,281,503 + actP b4067 0.14 -2.3 4,281,506 - actP b4067 0.14 +0.9 4,281,573 - actP b4067 0.18 +1.1 4,281,573 - actP b4067 0.18 -0.4 4,281,578 - actP b4067 0.18 -0.2 4,281,756 - actP b4067 0.29 -0.3 4,281,927 - actP b4067 0.39 -0.6 4,281,934 + actP b4067 0.40 -1.4 4,282,003 - actP b4067 0.44 -5.1 4,282,033 - actP b4067 0.46 +2.4 4,282,072 - actP b4067 0.48 -0.5 4,282,094 - actP b4067 0.50 -0.5 4,282,191 - actP b4067 0.55 -4.6 4,282,191 - actP b4067 0.55 +0.6 4,282,215 - actP b4067 0.57 -0.3 4,282,215 - actP b4067 0.57 -0.9 4,282,412 - actP b4067 0.69 -1.5 4,282,531 + actP b4067 0.76 -1.7 4,282,706 - actP b4067 0.87 +0.3 4,282,718 - actP b4067 0.87 -0.2 4,282,718 - actP b4067 0.87 -3.3 4,282,738 - actP b4067 0.89 +0.4 4,282,738 - actP b4067 0.89 -2.8 4,282,740 - actP b4067 0.89 +0.4 4,282,842 + -1.5 4,282,864 - -1.2 4,282,873 - -0.1 4,282,909 + -0.4 4,282,938 - -1.8 4,282,990 + yjcH b4068 0.22 -0.2 4,283,005 + yjcH b4068 0.26 +0.7 4,283,013 - yjcH b4068 0.29 -0.8 4,283,013 - yjcH b4068 0.29 +1.0 4,283,013 - yjcH b4068 0.29 +1.1 4,283,050 + yjcH b4068 0.41 +0.6 4,283,121 - yjcH b4068 0.63 -0.3 4,283,212 - +0.3 4,283,212 - -0.9 4,283,218 + +0.8 4,283,224 - +1.6 4,283,224 - -4.3 4,283,291 - +0.5 4,283,315 - +0.5 4,283,315 - +0.3 4,283,395 + -3.1 4,283,395 + +0.4 4,283,423 + -3.7 4,283,423 + -0.9 4,283,514 + -0.0 4,283,514 + -2.7 4,283,595 - +0.1 4,283,678 - acs b4069 0.12 -1.0 4,283,678 - acs b4069 0.12 -3.1 4,283,712 + acs b4069 0.14 -1.1 4,283,749 - acs b4069 0.16 -1.6 4,283,808 + acs b4069 0.19 -0.5 4,283,855 + acs b4069 0.21 -0.2 4,283,855 + acs b4069 0.21 -2.1 4,283,865 + acs b4069 0.22 -0.6
Or see this region's nucleotide sequence