Strain Fitness in Pseudomonas putida KT2440 around PP_4674

Experiment: P. putida South MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4673 and PP_4674 overlap by 4 nucleotidesPP_4674 and PP_5SG are separated by 185 nucleotidesPP_5SG and PP_23SG are separated by 131 nucleotides PP_4673: PP_4673 - Chi activated ATP-dependent DNA helicase and dsDNA/ssDNA exonuclease, at 5,300,098 to 5,303,772 _4673 PP_4674: PP_4674 - ATP-dependent DNA helicase and dsDNA/ssDNA exonuclease, ssDNA endonuclease, recombinase subunit, at 5,303,769 to 5,307,251 _4674 PP_5SG: PP_5SG - 5S ribosomal RNA, at 5,307,437 to 5,307,556 _5SG PP_23SG: PP_23SG - 23S ribosomal RNA, at 5,307,688 to 5,310,589 _23SG Position (kb) 5303 5304 5305 5306 5307 5308Strain fitness (log2 ratio) -1 0 1at 5307.294 kb on - strandat 5307.302 kb on + strandat 5307.303 kb on - strandat 5307.303 kb on - strandat 5307.303 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. putida South MOI 0.1
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5,307,294 - -0.1
5,307,302 + -1.3
5,307,303 - -0.6
5,307,303 - -0.2
5,307,303 - -0.3

Or see this region's nucleotide sequence